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Protein

Sensor histidine kinase MalK

Gene

malK

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Member of a two-component regulatory system MalK/MalR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates MalR by phosphorylation.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU31520-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase MalK (EC:2.7.13.3)
Alternative name(s):
Malate kinase sensor
Gene namesi
Name:malK
Synonyms:yufL
Ordered Locus Names:BSU31520
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence analysis
Transmembranei10 – 3223HelicalSequence analysisAdd
BLAST
Topological domaini33 – 172140ExtracellularSequence analysisAdd
BLAST
Transmembranei173 – 19523HelicalSequence analysisAdd
BLAST
Topological domaini196 – 533338CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Sensor histidine kinase MalKPRO_0000074804Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei338 – 3381Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO05250.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100017131.

Structurei

3D structure databases

ProteinModelPortaliO05250.
SMRiO05250. Positions 36-168, 200-314, 396-528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini210 – 28172PASAdd
BLAST
Domaini335 – 527193Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3290. LUCA.
HOGENOMiHOG000241936.
InParanoidiO05250.
KOiK11614.
OMAiHYQNGWL.
PhylomeDBiO05250.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR033463. sCache_3.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00989. PAS. 1 hit.
PF17203. sCache_3_2. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF55890. SSF55890. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O05250-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKTLKLQTR LTIFVCIVVL IALLITFFTV GAQTTKRIRD QEKATALQTA
60 70 80 90 100
EMVAEAPMTA AALESGKKQK ELQSYTKRVQ KITGTEFVVV MDMNGIRKTH
110 120 130 140 150
PDPSKIGKKF RGGDESEVLK GHVHISTASG TLGKSQRAFV PVYAENGKQV
160 170 180 190 200
GAVAVGITVN EIDEVISHSL RPLYFIICVS IFVGVIGAVI VARTVKNIMY
210 220 230 240 250
GLEPYEIATL LEERSAMLES TKEGILAVDE HGKIKLANAE AKRLFVKMGI
260 270 280 290 300
NTNPIDQDVD DILPKSRLKK VIETKKPLQD RDVRINGLEL VFNEVPIQLK
310 320 330 340 350
GQTVGAIATF RDKTEVKHLA EQLSGVKMYA NALRAQSHEF MNKLHVILGL
360 370 380 390 400
VQLKEYDDLG DYIKDIAIQQ KSETSEIIND VKSSVLAGFL LGKQSFIREQ
410 420 430 440 450
GANLDIECNG VIPNAADPSV IHELITIIGN LINNGLDAVA DMPKKQITMS
460 470 480 490 500
MRFHNSILDI EITDTGAGMS EEDQAKVFEQ GYSTKGKNRG FGLYFTQQSI
510 520 530
ENLKGQMILT SEKNEGTTFS IRIPYEPKEE NHD
Length:533
Mass (Da):58,930
Last modified:July 1, 1997 - v1
Checksum:i9CDF9A58EFD5AA10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93937 Genomic DNA. Translation: CAB07946.1.
AL009126 Genomic DNA. Translation: CAB15141.1.
PIRiA70009.
RefSeqiNP_391030.1. NC_000964.3.
WP_003228835.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15141; CAB15141; BSU31520.
GeneIDi937173.
KEGGibsu:BSU31520.
PATRICi18978274. VBIBacSub10457_3300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93937 Genomic DNA. Translation: CAB07946.1.
AL009126 Genomic DNA. Translation: CAB15141.1.
PIRiA70009.
RefSeqiNP_391030.1. NC_000964.3.
WP_003228835.1. NZ_JNCM01000033.1.

3D structure databases

ProteinModelPortaliO05250.
SMRiO05250. Positions 36-168, 200-314, 396-528.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100017131.

Proteomic databases

PaxDbiO05250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15141; CAB15141; BSU31520.
GeneIDi937173.
KEGGibsu:BSU31520.
PATRICi18978274. VBIBacSub10457_3300.

Phylogenomic databases

eggNOGiCOG3290. LUCA.
HOGENOMiHOG000241936.
InParanoidiO05250.
KOiK11614.
OMAiHYQNGWL.
PhylomeDBiO05250.

Enzyme and pathway databases

BioCyciBSUB:BSU31520-MONOMER.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR033463. sCache_3.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00989. PAS. 1 hit.
PF17203. sCache_3_2. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF55890. SSF55890. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMALK_BACSU
AccessioniPrimary (citable) accession number: O05250
Secondary accession number(s): Q795M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 1, 1997
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.