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Protein

Probable oligo-1,6-glucosidase 3

Gene

yugT

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei199 – 1991NucleophileBy similarity
Active sitei256 – 2561Proton donorBy similarity
Sitei326 – 3261Transition state stabilizerBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciBSUB:BSU31290-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable oligo-1,6-glucosidase 3 (EC:3.2.1.10)
Alternative name(s):
Oligosaccharide alpha-1,6-glucosidase 3
Sucrase-isomaltase 3
Short name:
Isomaltase 3
Gene namesi
Name:yugT
Ordered Locus Names:BSU31290
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU31290.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Probable oligo-1,6-glucosidase 3PRO_0000361649Add
BLAST

Proteomic databases

PaxDbiO05242.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU31290.

Structurei

3D structure databases

ProteinModelPortaliO05242.
SMRiO05242. Positions 2-553.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiCOG0366.
HOGENOMiHOG000220641.
InParanoidiO05242.
KOiK01187.
OMAiDISDYHA.
OrthoDBiEOG6RVFV2.
PhylomeDBiO05242.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

O05242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKAWWKEAV VYQIYPRSFK DSNGDGIGDI QGIRTKLSYI KELGADVIWI
60 70 80 90 100
CPLYDSPNAD NGYDIRDYQN ILSEFGTMED FDELLGDIHD LDMKLIMDLV
110 120 130 140 150
VNHTSDEHPW FIESRSSIHS EKRDWYIWKD GKNGKTPNNW ESIFGGPAWE
160 170 180 190 200
YDQKTSQYYL HLFDKKQPDL NWENEKVRNA VYDMINWWLD KGIDGFRVDA
210 220 230 240 250
ITHIKKKEGF PDMPNPKGLD YVPSFPYHMN ADGIMDLLTE LKENTFSRYP
260 270 280 290 300
IMTVGEANGV AAKEAADWAG EKNGIFSMIF QFEHLGLWDV EINESIDIVA
310 320 330 340 350
FKRILTDWQD SLEGIGWNAL FMENHDQPRS VSVWGDDGVY LKESAKALSA
360 370 380 390 400
VYFLMKGTPF IYQGQELGMT NVAFPSIEDY DDVALKRLYE TKTAKGTSHE
410 420 430 440 450
DVMKIVWKKG RDNSRTPMQW NAGPYAGFSE AKPWIGINEN YKWLNAEAQK
460 470 480 490 500
NDKTSVYHFY KSLIKLRQTY DVFINGTYEL ILPEDQQIFA YLRKNESHTA
510 520 530 540 550
LIAANLTGTP ALFRHSGLPL SSDALVLSNI ETEPHKHMTS VLLKPYEARI

YLWC
Length:554
Mass (Da):63,969
Last modified:July 28, 2009 - v2
Checksum:i67AD34910C96B6B7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti262 – 2621A → P in CAB07926 (PubMed:9274030).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93935 Genomic DNA. Translation: CAB07926.1.
AL009126 Genomic DNA. Translation: CAB15118.2.
PIRiH70011.
RefSeqiNP_391007.2. NC_000964.3.
WP_003243842.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15118; CAB15118; BSU31290.
GeneIDi938857.
KEGGibsu:BSU31290.
PATRICi18978222. VBIBacSub10457_3274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93935 Genomic DNA. Translation: CAB07926.1.
AL009126 Genomic DNA. Translation: CAB15118.2.
PIRiH70011.
RefSeqiNP_391007.2. NC_000964.3.
WP_003243842.1. NZ_JNCM01000033.1.

3D structure databases

ProteinModelPortaliO05242.
SMRiO05242. Positions 2-553.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU31290.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbiO05242.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15118; CAB15118; BSU31290.
GeneIDi938857.
KEGGibsu:BSU31290.
PATRICi18978222. VBIBacSub10457_3274.

Organism-specific databases

GenoListiBSU31290.

Phylogenomic databases

eggNOGiCOG0366.
HOGENOMiHOG000220641.
InParanoidiO05242.
KOiK01187.
OMAiDISDYHA.
OrthoDBiEOG6RVFV2.
PhylomeDBiO05242.

Enzyme and pathway databases

BioCyciBSUB:BSU31290-MONOMER.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)."
    Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T.
    Microbiology 143:2769-2774(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 262.

Entry informationi

Entry nameiO16G3_BACSU
AccessioniPrimary (citable) accession number: O05242
Secondary accession number(s): Q795N0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: July 28, 2009
Last modified: May 27, 2015
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.