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O05242 (O16G3_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable oligo-1,6-glucosidase 3

EC=3.2.1.10
Alternative name(s):
Oligosaccharide alpha-1,6-glucosidase 3
Sucrase-isomaltase 3
Short name=Isomaltase 3
Gene names
Name:yugT
Ordered Locus Names:BSU31290
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncation binding

Inferred from electronic annotation. Source: InterPro

oligo-1,6-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554Probable oligo-1,6-glucosidase 3
PRO_0000361649

Sites

Active site1991Nucleophile By similarity
Active site2561Proton donor By similarity
Site3261Transition state stabilizer By similarity

Experimental info

Sequence conflict2621A → P in CAB07926. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O05242 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 67AD34910C96B6B7

FASTA55463,969
        10         20         30         40         50         60 
MKKAWWKEAV VYQIYPRSFK DSNGDGIGDI QGIRTKLSYI KELGADVIWI CPLYDSPNAD 

        70         80         90        100        110        120 
NGYDIRDYQN ILSEFGTMED FDELLGDIHD LDMKLIMDLV VNHTSDEHPW FIESRSSIHS 

       130        140        150        160        170        180 
EKRDWYIWKD GKNGKTPNNW ESIFGGPAWE YDQKTSQYYL HLFDKKQPDL NWENEKVRNA 

       190        200        210        220        230        240 
VYDMINWWLD KGIDGFRVDA ITHIKKKEGF PDMPNPKGLD YVPSFPYHMN ADGIMDLLTE 

       250        260        270        280        290        300 
LKENTFSRYP IMTVGEANGV AAKEAADWAG EKNGIFSMIF QFEHLGLWDV EINESIDIVA 

       310        320        330        340        350        360 
FKRILTDWQD SLEGIGWNAL FMENHDQPRS VSVWGDDGVY LKESAKALSA VYFLMKGTPF 

       370        380        390        400        410        420 
IYQGQELGMT NVAFPSIEDY DDVALKRLYE TKTAKGTSHE DVMKIVWKKG RDNSRTPMQW 

       430        440        450        460        470        480 
NAGPYAGFSE AKPWIGINEN YKWLNAEAQK NDKTSVYHFY KSLIKLRQTY DVFINGTYEL 

       490        500        510        520        530        540 
ILPEDQQIFA YLRKNESHTA LIAANLTGTP ALFRHSGLPL SSDALVLSNI ETEPHKHMTS 

       550 
VLLKPYEARI YLWC 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)."
Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T.
Microbiology 143:2769-2774(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 262.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z93935 Genomic DNA. Translation: CAB07926.1.
AL009126 Genomic DNA. Translation: CAB15118.2.
PIRH70011.
RefSeqNP_391007.2. NC_000964.3.

3D structure databases

ProteinModelPortalO05242.
SMRO05242. Positions 2-553.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU31290.

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbO05242.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15118; CAB15118; BSU31290.
GeneID938857.
KEGGbsu:BSU31290.
PATRIC18978222. VBIBacSub10457_3274.

Organism-specific databases

GenoListBSU31290.

Phylogenomic databases

eggNOGCOG0366.
HOGENOMHOG000220641.
KOK01187.
OrthoDBEOG6RVFV2.
ProtClustDBCLSK887787.

Enzyme and pathway databases

BioCycBSUB:BSU31290-MONOMER.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameO16G3_BACSU
AccessionPrimary (citable) accession number: O05242
Secondary accession number(s): Q795N0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: July 28, 2009
Last modified: November 13, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList