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O05242

- O16G3_BACSU

UniProt

O05242 - O16G3_BACSU

Protein

Probable oligo-1,6-glucosidase 3

Gene

yugT

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei199 – 1991NucleophileBy similarity
    Active sitei256 – 2561Proton donorBy similarity
    Sitei326 – 3261Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. cation binding Source: InterPro
    2. oligo-1,6-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciBSUB:BSU31290-MONOMER.

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable oligo-1,6-glucosidase 3 (EC:3.2.1.10)
    Alternative name(s):
    Oligosaccharide alpha-1,6-glucosidase 3
    Sucrase-isomaltase 3
    Short name:
    Isomaltase 3
    Gene namesi
    Name:yugT
    Ordered Locus Names:BSU31290
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU31290.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 554554Probable oligo-1,6-glucosidase 3PRO_0000361649Add
    BLAST

    Proteomic databases

    PaxDbiO05242.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU31290.

    Structurei

    3D structure databases

    ProteinModelPortaliO05242.
    SMRiO05242. Positions 2-553.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Phylogenomic databases

    eggNOGiCOG0366.
    HOGENOMiHOG000220641.
    KOiK01187.
    OrthoDBiEOG6RVFV2.
    PhylomeDBiO05242.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O05242-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKAWWKEAV VYQIYPRSFK DSNGDGIGDI QGIRTKLSYI KELGADVIWI    50
    CPLYDSPNAD NGYDIRDYQN ILSEFGTMED FDELLGDIHD LDMKLIMDLV 100
    VNHTSDEHPW FIESRSSIHS EKRDWYIWKD GKNGKTPNNW ESIFGGPAWE 150
    YDQKTSQYYL HLFDKKQPDL NWENEKVRNA VYDMINWWLD KGIDGFRVDA 200
    ITHIKKKEGF PDMPNPKGLD YVPSFPYHMN ADGIMDLLTE LKENTFSRYP 250
    IMTVGEANGV AAKEAADWAG EKNGIFSMIF QFEHLGLWDV EINESIDIVA 300
    FKRILTDWQD SLEGIGWNAL FMENHDQPRS VSVWGDDGVY LKESAKALSA 350
    VYFLMKGTPF IYQGQELGMT NVAFPSIEDY DDVALKRLYE TKTAKGTSHE 400
    DVMKIVWKKG RDNSRTPMQW NAGPYAGFSE AKPWIGINEN YKWLNAEAQK 450
    NDKTSVYHFY KSLIKLRQTY DVFINGTYEL ILPEDQQIFA YLRKNESHTA 500
    LIAANLTGTP ALFRHSGLPL SSDALVLSNI ETEPHKHMTS VLLKPYEARI 550
    YLWC 554
    Length:554
    Mass (Da):63,969
    Last modified:July 28, 2009 - v2
    Checksum:i67AD34910C96B6B7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti262 – 2621A → P in CAB07926. (PubMed:9274030)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z93935 Genomic DNA. Translation: CAB07926.1.
    AL009126 Genomic DNA. Translation: CAB15118.2.
    PIRiH70011.
    RefSeqiNP_391007.2. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15118; CAB15118; BSU31290.
    GeneIDi938857.
    KEGGibsu:BSU31290.
    PATRICi18978222. VBIBacSub10457_3274.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z93935 Genomic DNA. Translation: CAB07926.1 .
    AL009126 Genomic DNA. Translation: CAB15118.2 .
    PIRi H70011.
    RefSeqi NP_391007.2. NC_000964.3.

    3D structure databases

    ProteinModelPortali O05242.
    SMRi O05242. Positions 2-553.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU31290.

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PaxDbi O05242.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15118 ; CAB15118 ; BSU31290 .
    GeneIDi 938857.
    KEGGi bsu:BSU31290.
    PATRICi 18978222. VBIBacSub10457_3274.

    Organism-specific databases

    GenoListi BSU31290.

    Phylogenomic databases

    eggNOGi COG0366.
    HOGENOMi HOG000220641.
    KOi K01187.
    OrthoDBi EOG6RVFV2.
    PhylomeDBi O05242.

    Enzyme and pathway databases

    BioCyci BSUB:BSU31290-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    [Graphical view ]
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)."
      Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T.
      Microbiology 143:2769-2774(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
      Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
      Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 262.

    Entry informationi

    Entry nameiO16G3_BACSU
    AccessioniPrimary (citable) accession number: O05242
    Secondary accession number(s): Q795N0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 93 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3