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Reviewed, UniProtKB/Swiss-Prot O05218 (YWRD_BACSU)

Last modified February 9, 2010. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Putative gamma-glutamyltransferase ywrD
    EC=2.3.2.2
Cleaved into the following 2 chains:
    1- Recommended name:
            Gamma-glutamyltranspeptidase large chain
    2- Recommended name:
            Gamma-glutamyltranspeptidase small chain
Gene names
Name: ywrD
Ordered Locus Names: BSU36100
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

(5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl amino acid.

Subunit structure

This enzyme consists of two polypeptide chains, which are synthesized from a single polypeptide By similarity.

Induction

Positively regulated by tnrA under nitrogen-limited conditions. Ref.3

Miscellaneous

Is not involved in the utilization of extracellular glutathione as a sulfur source.

Sequence similarities

Belongs to the gamma-glutamyltransferase family.

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Ontologies

Keywords
   Molecular functionAcyltransferase
Transferase
   PTMZymogen
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functionacyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-KW

gamma-glutamyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 338338Gamma-glutamyltranspeptidase large chain
PRO_0000360767
Chain339 – 525187Gamma-glutamyltranspeptidase small chain
PRO_0000360768

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Sequences

Sequence LengthMass (Da)Tools
O05218-1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 6E430B67757CC81A

FASTA52557,487
        10         20         30         40         50         60 
MNKSVIGTKQ MVVSPHYLAS QAGNRILDKG GNAFDAAVAV SACLAVVYPH MTGLGGDSFW 

        70         80         90        100        110        120 
LTFHQETKAV KVYNGSGRSG KNVTRDVYKG KSAIPLRGID SAITVPGMVD SWDAVLKEYG 

       130        140        150        160        170        180 
RLSLADVLEP ARDYAQNGFP VSADQCRHTE KNIELLASTP YTADIFTRRG KAPVPGERFV 

       190        200        210        220        230        240 
QKELADSLNL IAEKGRSAFY EGDLAQRIVS HLQNNGSYMT IDDFKAHRGE WAAPVSSDYR 

       250        260        270        280        290        300 
GYSVYQAPPN SQGFTGLLTL NILENYDFTQ IEHGSFEYYH VLVEALKKSF LDRDAVLTDP 

       310        320        330        340        350        360 
AFADIPLERL LDKRYAKQLA EEIGYLAIPA ESRPVGSDTA YAAVIDADGN AVSFIQSLYF 

       370        380        390        400        410        420 
EFGSAVTAGD TGILLQNRGS FFSLDENHVN TLEPRKRTFH TLMPAMVCKG GKPKILYGTQ 

       430        440        450        460        470        480 
GGEGQPQTQT AIITRMLDYG MHPQQAISEP RWVWGRTWGE EYEGLRVEGR FTDKTIQKLK 

       490        500        510        520 
DSGHLVEVVG DYDPLMGQAA AIKVDEEGFL QGGADPRGDG AAVGI

« Hide

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References

« Hide 'large scale' references
[1]"The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
Microbiology 143:3313-3328(1997) [PubMed: 9353933] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box."
Yoshida K., Yamaguchi H., Kinehara M., Ohki Y.-H., Nakaura Y., Fujita Y.
Mol. Microbiol. 49:157-165(2003) [PubMed: 12823818] [Abstract]
Cited for: INDUCTION BY TNRA.
[4]"Gamma-glutamyltranspeptidase, but not YwrD, is important in utilization of extracellular glutathione as a sulfur source in Bacillus subtilis."
Minami H., Suzuki H., Kumagai H.
J. Bacteriol. 186:1213-1214(2004) [PubMed: 14762019] [Abstract]
Cited for: LACK OF FUNCTION IN GLUTATHIONE METABOLISM.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z93767 Genomic DNA. Translation: CAB07790.1.
AL009126 Genomic DNA. Translation: CAB15627.1.
PIRF70068.
RefSeqNP_391491.1.

3D structure databases

HSSPHSSP built from PDB template 2DG5 based on UniProtKB P18956.
ModBaseSearch...

Protein family/group databases

MEROPST03.014.

Genome annotation databases

GeneID936884.
GenomeReviewsGene locus BSU36100 in contig AL009126_GR.
KEGGbsu:BSU36100.
NMPDRfig|224308.1.peg.3617.

Organism-specific databases

SubtiListBG12523. ywrD. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMHBG738311.
OMAILENYDF.
PhylomeDBO05218.

Enzyme and pathway databases

BioCycSUBTI:BSU36100-MONOMER.

Family and domain databases

InterProIPR000101. GGT_peptidase.
[Graphical view]
PANTHERPTHR11686. GGT_peptidase. 1 hit.
PfamPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSPR01210. GGTRANSPTASE.
TIGRFAMsTIGR00066. g_glut_trans. 1 hit.
PROSITEPS00462. G_GLU_TRANSPEPTIDASE. False negative.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameYWRD_BACSU
AccessionPrimary (citable) accession number: O05218
Secondary accession number(s): Q795C4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: July 1, 1997
Last modified: February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents