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Protein

Putative gamma-glutamyltransferase YwrD

Gene

ywrD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.
Glutathione + H2O = L-cysteinylglycine + L-glutamate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei339 – 3391NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Hydrolase, Protease, Transferase

Enzyme and pathway databases

BioCyciBSUB:BSU36100-MONOMER.

Protein family/group databases

MEROPSiT03.A09.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative gamma-glutamyltransferase YwrD (EC:2.3.2.2)
Alternative name(s):
Glutathione hydrolase (EC:3.4.19.13)
Cleaved into the following 2 chains:
Gene namesi
Name:ywrD
Ordered Locus Names:BSU36100
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338Gamma-glutamyltranspeptidase large chainPRO_0000360767Add
BLAST
Chaini339 – 525187Gamma-glutamyltranspeptidase small chainPRO_0000360768Add
BLAST

Post-translational modificationi

Cleaved by autocatalysis into a large and a small subunit.By similarity

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiO05218.

Expressioni

Inductioni

Positively regulated by TnrA under nitrogen-limited conditions.1 Publication

Interactioni

Subunit structurei

This enzyme consists of two polypeptide chains, which are synthesized from a single polypeptide.By similarity

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019521.

Structurei

3D structure databases

ProteinModelPortaliO05218.
SMRiO05218. Positions 2-525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the gamma-glutamyltransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105CFB. Bacteria.
COG0405. LUCA.
HOGENOMiHOG000175618.
InParanoidiO05218.
KOiK00681.
OMAiEPRWVWG.
PhylomeDBiO05218.

Family and domain databases

InterProiIPR000101. GGT_peptidase.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PANTHERiPTHR11686. PTHR11686. 1 hit.
PfamiPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSiPR01210. GGTRANSPTASE.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR00066. g_glut_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O05218-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKSVIGTKQ MVVSPHYLAS QAGNRILDKG GNAFDAAVAV SACLAVVYPH
60 70 80 90 100
MTGLGGDSFW LTFHQETKAV KVYNGSGRSG KNVTRDVYKG KSAIPLRGID
110 120 130 140 150
SAITVPGMVD SWDAVLKEYG RLSLADVLEP ARDYAQNGFP VSADQCRHTE
160 170 180 190 200
KNIELLASTP YTADIFTRRG KAPVPGERFV QKELADSLNL IAEKGRSAFY
210 220 230 240 250
EGDLAQRIVS HLQNNGSYMT IDDFKAHRGE WAAPVSSDYR GYSVYQAPPN
260 270 280 290 300
SQGFTGLLTL NILENYDFTQ IEHGSFEYYH VLVEALKKSF LDRDAVLTDP
310 320 330 340 350
AFADIPLERL LDKRYAKQLA EEIGYLAIPA ESRPVGSDTA YAAVIDADGN
360 370 380 390 400
AVSFIQSLYF EFGSAVTAGD TGILLQNRGS FFSLDENHVN TLEPRKRTFH
410 420 430 440 450
TLMPAMVCKG GKPKILYGTQ GGEGQPQTQT AIITRMLDYG MHPQQAISEP
460 470 480 490 500
RWVWGRTWGE EYEGLRVEGR FTDKTIQKLK DSGHLVEVVG DYDPLMGQAA
510 520
AIKVDEEGFL QGGADPRGDG AAVGI
Length:525
Mass (Da):57,487
Last modified:July 1, 1997 - v1
Checksum:i6E430B67757CC81A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93767 Genomic DNA. Translation: CAB07790.1.
AL009126 Genomic DNA. Translation: CAB15627.1.
PIRiF70068.
RefSeqiNP_391491.1. NC_000964.3.
WP_003227847.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB15627; CAB15627; BSU36100.
GeneIDi936884.
KEGGibsu:BSU36100.
PATRICi18979238. VBIBacSub10457_3781.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93767 Genomic DNA. Translation: CAB07790.1.
AL009126 Genomic DNA. Translation: CAB15627.1.
PIRiF70068.
RefSeqiNP_391491.1. NC_000964.3.
WP_003227847.1. NZ_JNCM01000034.1.

3D structure databases

ProteinModelPortaliO05218.
SMRiO05218. Positions 2-525.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019521.

Protein family/group databases

MEROPSiT03.A09.

Proteomic databases

PaxDbiO05218.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15627; CAB15627; BSU36100.
GeneIDi936884.
KEGGibsu:BSU36100.
PATRICi18979238. VBIBacSub10457_3781.

Phylogenomic databases

eggNOGiENOG4105CFB. Bacteria.
COG0405. LUCA.
HOGENOMiHOG000175618.
InParanoidiO05218.
KOiK00681.
OMAiEPRWVWG.
PhylomeDBiO05218.

Enzyme and pathway databases

BioCyciBSUB:BSU36100-MONOMER.

Family and domain databases

InterProiIPR000101. GGT_peptidase.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PANTHERiPTHR11686. PTHR11686. 1 hit.
PfamiPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSiPR01210. GGTRANSPTASE.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR00066. g_glut_trans. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYWRD_BACSU
AccessioniPrimary (citable) accession number: O05218
Secondary accession number(s): Q795C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: July 1, 1997
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Is not involved in the utilization of extracellular glutathione as a sulfur source.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.