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Protein

Type 2 DNA topoisomerase 6 subunit A

Gene

top6A

Organism
Sulfolobus shibatae
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Relaxes both positive and negative supercoils and exhibits a strong decatenase and unknotting activity; it cannot introduce DNA supercoils.1 Publication

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Not inhibited by the DNA gyrase inhibitor novobiocin, instead inhibited by eukaryotic topoisomerase inhibitors such as m- and o-amsacrine, ellipticine, and the quinolone CP-115,953 (PubMed:7961685).1 Publication

Temperature dependencei

Optimum temperature is 70-80 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei106 – 1061NucleophileUniRule annotation
Metal bindingi209 – 2091MagnesiumUniRule annotation
Metal bindingi261 – 2611MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi5.99.1.3. 6162.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 2 DNA topoisomerase 6 subunit AUniRule annotation (EC:5.99.1.3UniRule annotation1 Publication)
Alternative name(s):
Type II DNA topoisomerase VI subunit AUniRule annotation
Gene namesi
Name:top6AUniRule annotation
OrganismiSulfolobus shibatae
Taxonomic identifieri2286 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 389389Type 2 DNA topoisomerase 6 subunit APRO_0000145456Add
BLAST

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two Top6A and two Top6B chains.UniRule annotation2 Publications

Protein-protein interaction databases

DIPiDIP-29417N.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZBKX-ray3.56A/C/E/G1-389[»]
ProteinModelPortaliO05208.
SMRiO05208. Positions 10-389.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO05208.

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6A family.UniRule annotation

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00132. Top6A.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR004085. TopoVI_A.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 2 hits.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
PR01552. TPISMRASE6A.
SUPFAMiSSF56726. SSF56726. 1 hit.

Sequencei

Sequence statusi: Complete.

O05208-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSEFISKVD KEARRKAANI LRDKFLNLVE QLKKGEPLVM EIPMRTLSNA
60 70 80 90 100
IYDEKRKLLL LGEKKLRRNF LDLNEAKRFM QTVLMASIIY DALVSDEYPT
110 120 130 140 150
IRDLYYRGKH SLLLKSIEGN KIVSEENTWD EQKESDSVIV DIEVFTSLLR
160 170 180 190 200
EEMLILSKEK GKVVGNLRIR SGNDVIDLSK TGHGAYAIEP TPDLIDFIDV
210 220 230 240 250
DAEFVLVVEK DAVFQQLHRA GFWKQYKSIL ITSAGQPDRA TRRFVRRLNE
260 270 280 290 300
ELKLPVYILT DADPYGWYIF SVFRIGSISL SYESERLATP DAKFLGVSMG
310 320 330 340 350
DIFGNSRKKP YLSEAERKNY IIKAKDADIK RAEEIKNYEW FKTKAWQEEI
360 370 380
NTFLQRKAKL EIEAMASKGL KFLAFQYIPE KITNKDYIA
Length:389
Mass (Da):45,055
Last modified:July 1, 1997 - v1
Checksum:i6F617CB8231431B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10582 Genomic DNA. Translation: CAA71605.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10582 Genomic DNA. Translation: CAA71605.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZBKX-ray3.56A/C/E/G1-389[»]
ProteinModelPortaliO05208.
SMRiO05208. Positions 10-389.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29417N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi5.99.1.3. 6162.

Miscellaneous databases

EvolutionaryTraceiO05208.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00132. Top6A.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR004085. TopoVI_A.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 2 hits.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
PR01552. TPISMRASE6A.
SUPFAMiSSF56726. SSF56726. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "An atypical topoisomerase II from Archaea with implications for meiotic recombination."
    Bergerat A., de Massy B., Gadelle D., Varoutas P.-C., Nicolas A., Forterre P.
    Nature 386:414-417(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 212-221 AND 344-352.
    Strain: ATCC 51178 / DSM 5389 / JCM 8931 / NBRC 15437 / B12.
  2. "Purification of a DNA topoisomerase II from the hyperthermophilic archaeon Sulfolobus shibatae. A thermostable enzyme with both bacterial and eucaryal features."
    Bergerat A., Gadelle D., Forterre P.
    J. Biol. Chem. 269:27663-27669(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBUNIT.
    Strain: ATCC 51178 / DSM 5389 / JCM 8931 / NBRC 15437 / B12.
  3. "Crystal structure of an intact type II DNA topoisomerase: insights into DNA transfer mechanisms."
    Graille M., Cladiere L., Durand D., Lecointe F., Gadelle D., Quevillon-Cheruel S., Vachette P., Forterre P., van Tilbeurgh H.
    Structure 16:360-370(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.56 ANGSTROMS) IN COMPLEX WITH RADICICOL AND TOP6B, SUBUNIT.

Entry informationi

Entry nameiTOP6A_SULSH
AccessioniPrimary (citable) accession number: O05208
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: January 20, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.