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Protein

Type 2 DNA topoisomerase 6 subunit B

Gene

top6B

Organism
Sulfolobus shibatae
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Relaxes both positive and negative supercoils and exhibits a strong decatenase and unknotting activity; it cannot introduce DNA supercoils.1 Publication

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation1 Publication

Enzyme regulationi

Not inhibited by the DNA gyrase inhibitor novobiocin, instead inhibited by eukaryotic topoisomerase inhibitors such as m- and o-amsacrine, ellipticine, and the quinolone CP-115,953 (PubMed:7961685). Radicicol inhibits the ATPase activity (PubMed:16920739).2 Publications

Temperature dependencei

Optimum temperature is 70-80 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421ATP2 Publications
Binding sitei76 – 761ATP2 Publications
Binding sitei427 – 4271ATP1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi97 – 982ATP2 Publications
Nucleotide bindingi106 – 1138ATP2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi5.99.1.3. 6162.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 2 DNA topoisomerase 6 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation1 Publication)
Alternative name(s):
Type II DNA topoisomerase VI subunit BUniRule annotation
Short name:
TopoVI-BUniRule annotation
Gene namesi
Name:top6BUniRule annotation
OrganismiSulfolobus shibatae
Taxonomic identifieri2286 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Type 2 DNA topoisomerase 6 subunit BPRO_0000145470Add
BLAST

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two Top6A and two Top6B chains.UniRule annotation5 Publications

Protein-protein interaction databases

DIPiDIP-29416N.

Structurei

Secondary structure

1
530
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 187Combined sources
Helixi20 – 223Combined sources
Helixi28 – 4518Combined sources
Helixi48 – 503Combined sources
Beta strandi55 – 6410Combined sources
Turni65 – 684Combined sources
Beta strandi69 – 757Combined sources
Helixi83 – 853Combined sources
Helixi86 – 916Combined sources
Beta strandi92 – 976Combined sources
Beta strandi100 – 1023Combined sources
Turni109 – 1113Combined sources
Helixi112 – 12211Combined sources
Beta strandi127 – 1326Combined sources
Beta strandi137 – 14610Combined sources
Turni148 – 1503Combined sources
Beta strandi153 – 16210Combined sources
Beta strandi169 – 1779Combined sources
Helixi180 – 19718Combined sources
Beta strandi202 – 2065Combined sources
Beta strandi212 – 2154Combined sources
Helixi233 – 2353Combined sources
Helixi238 – 2447Combined sources
Helixi254 – 2585Combined sources
Beta strandi261 – 2644Combined sources
Helixi267 – 27610Combined sources
Helixi285 – 2873Combined sources
Helixi292 – 30211Combined sources
Helixi311 – 3133Combined sources
Helixi319 – 33012Combined sources
Beta strandi333 – 3397Combined sources
Beta strandi343 – 3453Combined sources
Beta strandi348 – 35912Combined sources
Beta strandi365 – 3673Combined sources
Beta strandi369 – 3746Combined sources
Beta strandi377 – 3793Combined sources
Helixi383 – 3853Combined sources
Helixi387 – 3948Combined sources
Helixi397 – 4004Combined sources
Beta strandi404 – 4074Combined sources
Beta strandi409 – 41911Combined sources
Beta strandi422 – 4243Combined sources
Beta strandi428 – 4303Combined sources
Helixi434 – 46835Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MU5X-ray2.00A2-470[»]
1MX0X-ray2.30A/B/C/D/E/F1-470[»]
1Z59X-ray2.10A2-470[»]
1Z5AX-ray2.20A/B2-470[»]
1Z5BX-ray2.00A/B2-470[»]
1Z5CX-ray2.20A/B2-470[»]
2HKJX-ray2.00A2-470[»]
2ZBKX-ray3.56B/D/F/H1-530[»]
ProteinModelPortaliO05207.
SMRiO05207. Positions 14-519.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO05207.

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6B family.UniRule annotation

Family and domain databases

CDDicd00823. TopoIIB_Trans. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
HAMAPiMF_00322. Top6B. 1 hit.
InterProiIPR003594. HATPase_C.
IPR010979. Ribosomal_S13-like_H2TH.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005734. TopoVI_B.
IPR015320. TopoVI_B_transducer.
[Graphical view]
PANTHERiPTHR10871:SF4. PTHR10871:SF4. 1 hit.
PfamiPF02518. HATPase_c. 1 hit.
PF09239. Topo-VIb_trans. 1 hit.
[Graphical view]
PIRSFiPIRSF006553. TopoVI_B. 1 hit.
ProDomiPD013790. TopoVI_B_transducer. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR01052. top6b. 1 hit.

Sequencei

Sequence statusi: Complete.

O05207-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAKEKFTSL SPAEFFKRNP ELAGFPNPAR ALYQTVRELI ENSLDATDVH
60 70 80 90 100
GILPNIKITI DLIDDARQIY KVNVVDNGIG IPPQEVPNAF GRVLYSSKYV
110 120 130 140 150
NRQTRGMYGL GVKAAVLYSQ MHQDKPIEIE TSPVNSKRIY TFKLKIDINK
160 170 180 190 200
NEPIIVERGS VENTRGFHGT SVAISIPGDW PKAKSRIYEY IKRTYIITPY
210 220 230 240 250
AEFIFKDPEG NVTYYPRLTN KIPKPPQEVK PHPYGVDREE IKILINNLKR
260 270 280 290 300
DYTIKEFLVN EFQSIGDTTA DKILELAGLK PNKKVKNLTE EEITRLVETF
310 320 330 340 350
KKDEDFRSPS ADSLSVIGED LIELGLKKIF NPDFAASITR KPKAYQGHPF
360 370 380 390 400
IVEAGVAFGG SIPVGEEPIV LRYANKIPLI YDEKSDVIWK VVEELDWKRY
410 420 430 440 450
GIESDQYQMV VMVHLCSTKI PYKSAGKESI AEVENIEKEI KNALMEVARK
460 470 480 490 500
LKQYLSEKRK EQEAKKKLLA YLKYIPEVSR SLATFLASGN KELVSKYQNE
510 520 530
ISEGLFKLIS KKLDLINIEE YRKVYRVDSE
Length:530
Mass (Da):60,528
Last modified:July 1, 1997 - v1
Checksum:i772F221FCD28441A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10582 Genomic DNA. Translation: CAA71604.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10582 Genomic DNA. Translation: CAA71604.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MU5X-ray2.00A2-470[»]
1MX0X-ray2.30A/B/C/D/E/F1-470[»]
1Z59X-ray2.10A2-470[»]
1Z5AX-ray2.20A/B2-470[»]
1Z5BX-ray2.00A/B2-470[»]
1Z5CX-ray2.20A/B2-470[»]
2HKJX-ray2.00A2-470[»]
2ZBKX-ray3.56B/D/F/H1-530[»]
ProteinModelPortaliO05207.
SMRiO05207. Positions 14-519.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29416N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi5.99.1.3. 6162.

Miscellaneous databases

EvolutionaryTraceiO05207.

Family and domain databases

CDDicd00823. TopoIIB_Trans. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
HAMAPiMF_00322. Top6B. 1 hit.
InterProiIPR003594. HATPase_C.
IPR010979. Ribosomal_S13-like_H2TH.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005734. TopoVI_B.
IPR015320. TopoVI_B_transducer.
[Graphical view]
PANTHERiPTHR10871:SF4. PTHR10871:SF4. 1 hit.
PfamiPF02518. HATPase_c. 1 hit.
PF09239. Topo-VIb_trans. 1 hit.
[Graphical view]
PIRSFiPIRSF006553. TopoVI_B. 1 hit.
ProDomiPD013790. TopoVI_B_transducer. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR01052. top6b. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTOP6B_SULSH
AccessioniPrimary (citable) accession number: O05207
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.