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Protein

Type 2 DNA topoisomerase 6 subunit B

Gene

top6B

Organism
Sulfolobus shibatae
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Relaxes both positive and negative supercoils and exhibits a strong decatenase and unknotting activity; it cannot introduce DNA supercoils.1 Publication

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation1 Publication

Enzyme regulationi

Not inhibited by the DNA gyrase inhibitor novobiocin, instead inhibited by eukaryotic topoisomerase inhibitors such as m- and o-amsacrine, ellipticine, and the quinolone CP-115,953 (PubMed:7961685). Radicicol inhibits the ATPase activity (PubMed:16920739).2 Publications

Temperature dependencei

Optimum temperature is 70-80 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42ATP2 Publications1
Binding sitei76ATP2 Publications1
Binding sitei427ATP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi97 – 98ATP2 Publications2
Nucleotide bindingi106 – 113ATP2 Publications8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi5.99.1.3. 6162.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 2 DNA topoisomerase 6 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation1 Publication)
Alternative name(s):
Type II DNA topoisomerase VI subunit BUniRule annotation
Short name:
TopoVI-BUniRule annotation
Gene namesi
Name:top6BUniRule annotation
OrganismiSulfolobus shibatae
Taxonomic identifieri2286 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001454701 – 530Type 2 DNA topoisomerase 6 subunit BAdd BLAST530

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two Top6A and two Top6B chains.UniRule annotation5 Publications

Protein-protein interaction databases

DIPiDIP-29416N.

Structurei

Secondary structure

1530
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 18Combined sources7
Helixi20 – 22Combined sources3
Helixi28 – 45Combined sources18
Helixi48 – 50Combined sources3
Beta strandi55 – 64Combined sources10
Turni65 – 68Combined sources4
Beta strandi69 – 75Combined sources7
Helixi83 – 85Combined sources3
Helixi86 – 91Combined sources6
Beta strandi92 – 97Combined sources6
Beta strandi100 – 102Combined sources3
Turni109 – 111Combined sources3
Helixi112 – 122Combined sources11
Beta strandi127 – 132Combined sources6
Beta strandi137 – 146Combined sources10
Turni148 – 150Combined sources3
Beta strandi153 – 162Combined sources10
Beta strandi169 – 177Combined sources9
Helixi180 – 197Combined sources18
Beta strandi202 – 206Combined sources5
Beta strandi212 – 215Combined sources4
Helixi233 – 235Combined sources3
Helixi238 – 244Combined sources7
Helixi254 – 258Combined sources5
Beta strandi261 – 264Combined sources4
Helixi267 – 276Combined sources10
Helixi285 – 287Combined sources3
Helixi292 – 302Combined sources11
Helixi311 – 313Combined sources3
Helixi319 – 330Combined sources12
Beta strandi333 – 339Combined sources7
Beta strandi343 – 345Combined sources3
Beta strandi348 – 359Combined sources12
Beta strandi365 – 367Combined sources3
Beta strandi369 – 374Combined sources6
Beta strandi377 – 379Combined sources3
Helixi383 – 385Combined sources3
Helixi387 – 394Combined sources8
Helixi397 – 400Combined sources4
Beta strandi404 – 407Combined sources4
Beta strandi409 – 419Combined sources11
Beta strandi422 – 424Combined sources3
Beta strandi428 – 430Combined sources3
Helixi434 – 468Combined sources35

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MU5X-ray2.00A2-470[»]
1MX0X-ray2.30A/B/C/D/E/F1-470[»]
1Z59X-ray2.10A2-470[»]
1Z5AX-ray2.20A/B2-470[»]
1Z5BX-ray2.00A/B2-470[»]
1Z5CX-ray2.20A/B2-470[»]
2HKJX-ray2.00A2-470[»]
2ZBKX-ray3.56B/D/F/H1-530[»]
ProteinModelPortaliO05207.
SMRiO05207.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO05207.

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6B family.UniRule annotation

Family and domain databases

CDDicd00823. TopoIIB_Trans. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
HAMAPiMF_00322. Top6B. 1 hit.
InterProiIPR003594. HATPase_C.
IPR010979. Ribosomal_S13-like_H2TH.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005734. TopoVI_B.
IPR015320. TopoVI_B_transducer.
[Graphical view]
PANTHERiPTHR10871:SF4. PTHR10871:SF4. 1 hit.
PfamiPF02518. HATPase_c. 1 hit.
PF09239. Topo-VIb_trans. 1 hit.
[Graphical view]
PIRSFiPIRSF006553. TopoVI_B. 1 hit.
ProDomiPD013790. TopoVI_B_transducer. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR01052. top6b. 1 hit.

Sequencei

Sequence statusi: Complete.

O05207-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAKEKFTSL SPAEFFKRNP ELAGFPNPAR ALYQTVRELI ENSLDATDVH
60 70 80 90 100
GILPNIKITI DLIDDARQIY KVNVVDNGIG IPPQEVPNAF GRVLYSSKYV
110 120 130 140 150
NRQTRGMYGL GVKAAVLYSQ MHQDKPIEIE TSPVNSKRIY TFKLKIDINK
160 170 180 190 200
NEPIIVERGS VENTRGFHGT SVAISIPGDW PKAKSRIYEY IKRTYIITPY
210 220 230 240 250
AEFIFKDPEG NVTYYPRLTN KIPKPPQEVK PHPYGVDREE IKILINNLKR
260 270 280 290 300
DYTIKEFLVN EFQSIGDTTA DKILELAGLK PNKKVKNLTE EEITRLVETF
310 320 330 340 350
KKDEDFRSPS ADSLSVIGED LIELGLKKIF NPDFAASITR KPKAYQGHPF
360 370 380 390 400
IVEAGVAFGG SIPVGEEPIV LRYANKIPLI YDEKSDVIWK VVEELDWKRY
410 420 430 440 450
GIESDQYQMV VMVHLCSTKI PYKSAGKESI AEVENIEKEI KNALMEVARK
460 470 480 490 500
LKQYLSEKRK EQEAKKKLLA YLKYIPEVSR SLATFLASGN KELVSKYQNE
510 520 530
ISEGLFKLIS KKLDLINIEE YRKVYRVDSE
Length:530
Mass (Da):60,528
Last modified:July 1, 1997 - v1
Checksum:i772F221FCD28441A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10582 Genomic DNA. Translation: CAA71604.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10582 Genomic DNA. Translation: CAA71604.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MU5X-ray2.00A2-470[»]
1MX0X-ray2.30A/B/C/D/E/F1-470[»]
1Z59X-ray2.10A2-470[»]
1Z5AX-ray2.20A/B2-470[»]
1Z5BX-ray2.00A/B2-470[»]
1Z5CX-ray2.20A/B2-470[»]
2HKJX-ray2.00A2-470[»]
2ZBKX-ray3.56B/D/F/H1-530[»]
ProteinModelPortaliO05207.
SMRiO05207.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29416N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi5.99.1.3. 6162.

Miscellaneous databases

EvolutionaryTraceiO05207.

Family and domain databases

CDDicd00823. TopoIIB_Trans. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
HAMAPiMF_00322. Top6B. 1 hit.
InterProiIPR003594. HATPase_C.
IPR010979. Ribosomal_S13-like_H2TH.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005734. TopoVI_B.
IPR015320. TopoVI_B_transducer.
[Graphical view]
PANTHERiPTHR10871:SF4. PTHR10871:SF4. 1 hit.
PfamiPF02518. HATPase_c. 1 hit.
PF09239. Topo-VIb_trans. 1 hit.
[Graphical view]
PIRSFiPIRSF006553. TopoVI_B. 1 hit.
ProDomiPD013790. TopoVI_B_transducer. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR01052. top6b. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTOP6B_SULSH
AccessioniPrimary (citable) accession number: O05207
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.