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Protein

Glycyl-glycine endopeptidase ALE-1

Gene
N/A
Organism
Staphylococcus capitis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.

Catalytic activityi

Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

pH dependencei

Optimum pH is 7-9.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi150ZincCurated1
Metal bindingi154ZincCurated1
Active sitei231By similarity1
Metal bindingi233ZincCurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM23.012.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycyl-glycine endopeptidase ALE-1 (EC:3.4.24.75)
Alternative name(s):
Staphylolytic enzyme ALE-1
OrganismiStaphylococcus capitis
Taxonomic identifieri29388 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Add BLAST35
ChainiPRO_000002681436 – 362Glycyl-glycine endopeptidase ALE-1Add BLAST327

Structurei

Secondary structure

1362
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi279 – 291Combined sources13
Beta strandi293 – 300Combined sources8
Beta strandi306 – 310Combined sources5
Beta strandi315 – 324Combined sources10
Beta strandi327 – 333Combined sources7
Beta strandi339 – 347Combined sources9
Turni349 – 351Combined sources3
Beta strandi358 – 362Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R77X-ray1.75A/B271-362[»]
ProteinModelPortaliO05156.
SMRiO05156.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO05156.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini282 – 350SH3bPROSITE-ProRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the peptidase M23B family.Curated
Contains 1 SH3b domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

InterProiIPR011055. Dup_hybrid_motif.
IPR016047. Peptidase_M23.
IPR003646. SH3-like_bac-type.
[Graphical view]
PfamiPF01551. Peptidase_M23. 1 hit.
PF08460. SH3_5. 1 hit.
[Graphical view]
SMARTiSM00287. SH3b. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
PROSITEiPS51781. SH3B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O05156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTNRKFTLV KSLSIGLGTF LVGSVFLTVN DEASASTKVD APKVEQEAPA
60 70 80 90 100
KADAPKVEQE APAKADAPKV EQEAPAKVDA PKVEQEAPAK VDAPKVEQEA
110 120 130 140 150
PAKADAPKVE QKRTFVREAA QSNHSASWLN NYKKGYGYGP YPLGINGGNH
160 170 180 190 200
YGVDFFMNVG TPVRAISDGK IVEAGWTNYG GGNEIGLVEN DGVHRQWYMH
210 220 230 240 250
LSKFNVKVGD RVKAGQIIGW SGSTGYSTAP HLHFQRMTNS FSNNTAQDPM
260 270 280 290 300
PFLKSAGYGS NSTSSSNNNG YKTNKYGTLY KSESASFTAN TDIITRLTGP
310 320 330 340 350
FRSMPQSGVL RKGLTIKYDE VMKQDGHVWV GYNTNSGKRV YLPVRTWNES
360
TGELGPLWGT IK
Length:362
Mass (Da):39,350
Last modified:July 1, 1997 - v1
Checksum:iC50DCDFDF9F6400E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86328 Genomic DNA. Translation: BAA13069.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86328 Genomic DNA. Translation: BAA13069.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R77X-ray1.75A/B271-362[»]
ProteinModelPortaliO05156.
SMRiO05156.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM23.012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO05156.

Family and domain databases

InterProiIPR011055. Dup_hybrid_motif.
IPR016047. Peptidase_M23.
IPR003646. SH3-like_bac-type.
[Graphical view]
PfamiPF01551. Peptidase_M23. 1 hit.
PF08460. SH3_5. 1 hit.
[Graphical view]
SMARTiSM00287. SH3b. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
PROSITEiPS51781. SH3B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALE1_STACP
AccessioniPrimary (citable) accession number: O05156
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.