Reviewed, UniProtKB/Swiss-Prot O05146 (MHPB_RHOGO)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
    EC=1.13.11.16

Gene names

Name:	mhpB
Synonyms:	hppB

Organism

Rhodococcus globerulus

Taxonomic identifier

33008 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity.
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653 2,3-dihydroxicinnamic acid + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653
Cofactor	Fe2+ ion By similarity.
Pathway	Aromatic compound metabolism; 3-phenylpropionic acid degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653		PRO_0000337665
Sites
	Active site	115	1	Proton donor By similarity
	Active site	179	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	O05146-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: D6A900C8AC2F8C0A	FASTA	314	34,735
	10 20 30 40 50 60 MKQALLCMSH SPLLHHLDPP ADVKASVEAA FDQARAFVHN FDPDVIVNFG PDHYNGFFYD 70 80 90 100 110 120 LMPPFCIGYK AKGSGDYDSF AGELNVPEAM AEDLAQFVMD QGLDIAISRQ MEVDHGAVQP 130 140 150 160 170 180 MEIIYGDVAS KPLIPVFVNS VARPFVKVAR VRKFGEAVGA YFKNSDKKVL FIGSGGLSHD 190 200 210 220 230 240 PPVPQIATAD EAQRKMLTDG RNPTPQARAA RQQRVIDTAV KFAADEADIM DLNPEWDRGF 250 260 270 280 290 300 LDVCASGRIE DFDRYTADDM DAVAGHSSHE VRNWVAAYSA LRACGEYEIA YEFYRPIKEY 310 ISGFAVTTAI LRDI

References

[1]	"A 3-(3-hydroxyphenyl)propionic acid catabolic pathway in Rhodococcus globerulus PWD1: cloning and characterization of the hpp operon." Barnes M.R., Duetz W.A., Williams P.A. J. Bacteriol. 179:6145-6153(1997) [PubMed: 9324265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PWD1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U89712 Genomic DNA. Translation: AAB81314.1.
3D structure databases
ModBase	Search...
Family and domain databases
HAMAP	MF_01653. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB_RHOGO

Accession

Primary (citable) accession number: O05146

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)