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Protein

Soluble pyridine nucleotide transhydrogenase

Gene

sthA

Organism
Pseudomonas fluorescens
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation.

Catalytic activityi

NADPH + NAD+ = NADP+ + NADH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi35 – 4410FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Soluble pyridine nucleotide transhydrogenase (EC:1.6.1.1)
Short name:
STH
Alternative name(s):
NAD(P)(+) transhydrogenase [B-specific]
Gene namesi
Name:sthA
Synonyms:sth
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 464463Soluble pyridine nucleotide transhydrogenasePRO_0000068069Add
BLAST

Interactioni

Subunit structurei

Homooligomer; probably composed of four stacked rings of 7 or 8 monomers. Forms filamentous structures.

Protein-protein interaction databases

STRINGi1114970.PSF113_3971.

Structurei

3D structure databases

ProteinModelPortaliO05139.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DX1. Bacteria.
COG1249. LUCA.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
HAMAPiMF_00247. SthA.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR022962. STH.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O05139-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVYNYDVVV LGSGPAGEGA AMNAAKAGRK VAMVDSRRQV GGNCTHLGTI
60 70 80 90 100
PSKALRHSVR QIMQFNTNPM FRAIGEPRWF SFPDVLKSAE KVISKQVASR
110 120 130 140 150
TGYYARNRVD LFFGTGSFAD EQTVEVVCAN GVVEKLVAKH IIIATGSRPY
160 170 180 190 200
RPADIDFHHP RIYDSDTILS LGHTPRKLII YGAGVIGCEY ASIFSGLGVL
210 220 230 240 250
VELVDNRDQL LSFLDSEISQ ALSYHFSNNN ITVRHNEEYD RVEGLDNGVI
260 270 280 290 300
LHLKSGKKIK ADALLWCNGR TGNTDKLGME NIGVKVNSRG QIEVDENYRT
310 320 330 340 350
CVTNIYGAGD VIGWPSLASA AHDQGRSAAG SIVDNGSWRY VNDVPTGIYT
360 370 380 390 400
IPEISSIGKN EHELTKAKVP YEVGKAFFKS MARAQIAGEP QGMLKILFHR
410 420 430 440 450
ETLEVLGVHC FGYQASEIVH IGQAIMNQPG EQNTLKYFVN TTFNYPTMAE
460
AYRVAAYDGL NRLF
Length:464
Mass (Da):51,008
Last modified:January 23, 2007 - v3
Checksum:i333D7F4EABB6944F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261K → D AA sequence (PubMed:9098078).Curated
Sequence conflicti26 – 261K → R AA sequence (PubMed:9098078).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91523 Genomic DNA. Translation: AAB50562.1.
RefSeqiWP_024074093.1. NZ_CP008896.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91523 Genomic DNA. Translation: AAB50562.1.
RefSeqiWP_024074093.1. NZ_CP008896.1.

3D structure databases

ProteinModelPortaliO05139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1114970.PSF113_3971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105DX1. Bacteria.
COG1249. LUCA.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
HAMAPiMF_00247. SthA.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR022962. STH.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning, sequence, and properties of the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens."
    French C.E., Boonstra B., Bufton K.A.J., Bruce N.C.
    J. Bacteriol. 179:2761-2765(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-26, CHARACTERIZATION.
    Strain: NCIMB 9815 / KCTC 1767.

Entry informationi

Entry nameiSTHA_PSEFL
AccessioniPrimary (citable) accession number: O05139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: November 11, 2015
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.