O05139 (STHA_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Soluble pyridine nucleotide transhydrogenase
Short name=STH
EC=1.6.1.1

Alternative name(s):
NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Synonyms:	sth

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation. HAMAP MF_00247
Catalytic activity	NADPH + NAD+ = NADP+ + NADH. HAMAP MF_00247
Cofactor	Binds 1 FAD per subunit By similarity. HAMAP MF_00247
Subunit structure	Homooligomer; probably composed of four stacked rings of 7 or 8 monomers. Forms filamentous structures.
Subcellular location	Cytoplasm HAMAP MF_00247.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 464	463	Soluble pyridine nucleotide transhydrogenase HAMAP MF_00247		PRO_0000068069
Regions
	Nucleotide binding	35 – 44	10	FAD By similarity
Experimental info
	Sequence conflict	26	1	K → D AA sequence Ref.1
	Sequence conflict	26	1	K → R AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O05139 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 333D7F4EABB6944F	FASTA	464	51,008
	10 20 30 40 50 60 MAVYNYDVVV LGSGPAGEGA AMNAAKAGRK VAMVDSRRQV GGNCTHLGTI PSKALRHSVR 70 80 90 100 110 120 QIMQFNTNPM FRAIGEPRWF SFPDVLKSAE KVISKQVASR TGYYARNRVD LFFGTGSFAD 130 140 150 160 170 180 EQTVEVVCAN GVVEKLVAKH IIIATGSRPY RPADIDFHHP RIYDSDTILS LGHTPRKLII 190 200 210 220 230 240 YGAGVIGCEY ASIFSGLGVL VELVDNRDQL LSFLDSEISQ ALSYHFSNNN ITVRHNEEYD 250 260 270 280 290 300 RVEGLDNGVI LHLKSGKKIK ADALLWCNGR TGNTDKLGME NIGVKVNSRG QIEVDENYRT 310 320 330 340 350 360 CVTNIYGAGD VIGWPSLASA AHDQGRSAAG SIVDNGSWRY VNDVPTGIYT IPEISSIGKN 370 380 390 400 410 420 EHELTKAKVP YEVGKAFFKS MARAQIAGEP QGMLKILFHR ETLEVLGVHC FGYQASEIVH 430 440 450 460 IGQAIMNQPG EQNTLKYFVN TTFNYPTMAE AYRVAAYDGL NRLF

References

[1]	"Cloning, sequence, and properties of the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens." French C.E., Boonstra B., Bufton K.A.J., Bruce N.C. J. Bacteriol. 179:2761-2765(1997) [PubMed: 9098078] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-26, CHARACTERIZATION. Strain: NCIMB 9815.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U91523 Genomic DNA. Translation: AAB50562.1.
3D structure databases
ProteinModelPortal	O05139.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00247. SthA. [Tree]
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. IPR022962. STH. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

STHA_PSEFL

Accession

Primary (citable) accession number: O05139

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 23, 2007
Last modified:	September 21, 2011
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program