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O05137 (MASZ_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Malate synthase G
EC=2.3.3.9
Gene names
Name:glcB
Synonyms:FC2.4
OrganismPseudomonas fluorescens
Taxonomic identifier294 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length725 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00641

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00641.

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 725725Malate synthase G HAMAP-Rule MF_00641
PRO_0000166892

Sites

Active site3401Proton acceptor By similarity
Active site6311Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
O05137 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 0C3326FE67C9B381

FASTA72578,861
        10         20         30         40         50         60 
MTEHVQVGGL QVAKVLFDFV NNEAIPGTGI TADQFWAGAD KVIHDLAPKN KALLAKRDDF 

        70         80         90        100        110        120 
QARIDTWHQT HAGQAHDPVA YKAFLQDIGY LLPEAADFQA STQNVDDEIA RMAGPQLVVP 

       130        140        150        160        170        180 
VMNARFALNA SNARWGSLYD ALYGTDAISE ADGAEKGKGY NKVRGDKVIA FARAFLDEAA 

       190        200        210        220        230        240 
PLSAGSHVDS TGYKIADGKL IVSLKGGSNS GLRDDAQLIG FQGPAAQPIA ILLKHNGLHF 

       250        260        270        280        290        300 
EIQIDASTPV GQTDAAGVKD VLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL 

       310        320        330        340        350        360 
AEEVAKGGKT FTRTMNPDRV YTGVDGQDVT LHGRSLLFVR NVGHLMTIDA ILDKAGNEVP 

       370        380        390        400        410        420 
EGILDGLLTS LAAIHSLNGN SSRKNSRTGS VYIVKPKMHG PEEAAFTNEL FGRIEDVLNL 

       430        440        450        460        470        480 
PRNTLKVGIM DEERRTTVNL KACIKAASER VVFINTGFLD RTGDEIHTSM EAGAMVRKAA 

       490        500        510        520        530        540 
MKTEKWIGAY ENWNVDIGLS TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV 

       550        560        570        580        590        600 
PSPTAAALHA LHYHKVDVFA RQAELAKRER ASVDDILTIP LAKNTDWSEE EIRNELDNNA 

       610        620        630        640        650        660 
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ HIANWLRHGV VTQDQVMESL 

       670        680        690        700        710        720 
KRMAPVVDRQ NAGDALYRPL APDFDSNIAF QAAVELVIEG TKQPNGYTEP VLHRKRREFK 


AKNGL

« Hide

References

[1]"A functional glyoxylate bypass is mandatory for utilization of alkanes by Pseudomonas fluorescens."
Artiguenave F.M., Delecu M., Vilagines R., Danglot C.
Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: L6.5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Y11998 Genomic DNA. Translation: CAA72726.1.

3D structure databases

ProteinModelPortalO05137.
SMRO05137. Positions 2-722.
ModBaseSearch...

2D gel databases

World-2DPAGE0008:O05137.

Proteomic databases

PRIDEO05137.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00703; UER00720.

Family and domain databases

Gene3D2.170.170.11. 2 hits.
HAMAPMF_00641. Malate_synth_G.
InterProIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PANTHERPTHR21631:SF1. PTHR21631:SF1. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMSSF51645. Malat_synth_like. 1 hit.
TIGRFAMsTIGR01345. malate_syn_G. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMASZ_PSEFL
AccessionPrimary (citable) accession number: O05137
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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