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O05137

- MASZ_PSEFL

UniProt

O05137 - MASZ_PSEFL

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Protein
Malate synthase G
Gene
glcB, FC2.4
Organism
Pseudomonas fluorescens
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygen By similarity
Binding sitei276 – 2761Acetyl-CoA By similarity
Binding sitei313 – 3131Acetyl-CoA By similarity
Active sitei340 – 3401Proton acceptor By similarity
Binding sitei340 – 3401Glyoxylate By similarity
Metal bindingi432 – 4321Magnesium By similarity
Binding sitei432 – 4321Glyoxylate By similarity
Metal bindingi460 – 4601Magnesium By similarity
Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygen By similarity
Active sitei631 – 6311Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G (EC:2.3.3.9)
Gene namesi
Name:glcB
Synonyms:FC2.4
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Malate synthase GUniRule annotation
PRO_0000166892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei617 – 6171Cysteine sulfenic acid (-SOH) By similarity

Keywords - PTMi

Oxidation

Proteomic databases

PRIDEiO05137.

2D gel databases

World-2DPAGE0008:O05137.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO05137.
SMRiO05137. Positions 2-722.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA binding By similarity
Regioni457 – 4604Glyoxylate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

O05137-1 [UniParc]FASTAAdd to Basket

« Hide

MTEHVQVGGL QVAKVLFDFV NNEAIPGTGI TADQFWAGAD KVIHDLAPKN    50
KALLAKRDDF QARIDTWHQT HAGQAHDPVA YKAFLQDIGY LLPEAADFQA 100
STQNVDDEIA RMAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDAISE 150
ADGAEKGKGY NKVRGDKVIA FARAFLDEAA PLSAGSHVDS TGYKIADGKL 200
IVSLKGGSNS GLRDDAQLIG FQGPAAQPIA ILLKHNGLHF EIQIDASTPV 250
GQTDAAGVKD VLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL 300
AEEVAKGGKT FTRTMNPDRV YTGVDGQDVT LHGRSLLFVR NVGHLMTIDA 350
ILDKAGNEVP EGILDGLLTS LAAIHSLNGN SSRKNSRTGS VYIVKPKMHG 400
PEEAAFTNEL FGRIEDVLNL PRNTLKVGIM DEERRTTVNL KACIKAASER 450
VVFINTGFLD RTGDEIHTSM EAGAMVRKAA MKTEKWIGAY ENWNVDIGLS 500
TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV PSPTAAALHA 550
LHYHKVDVFA RQAELAKRER ASVDDILTIP LAKNTDWSEE EIRNELDNNA 600
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ HIANWLRHGV 650
VTQDQVMESL KRMAPVVDRQ NAGDALYRPL APDFDSNIAF QAAVELVIEG 700
TKQPNGYTEP VLHRKRREFK AKNGL 725
Length:725
Mass (Da):78,861
Last modified:July 1, 1997 - v1
Checksum:i0C3326FE67C9B381
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y11998 Genomic DNA. Translation: CAA72726.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y11998 Genomic DNA. Translation: CAA72726.1 .

3D structure databases

ProteinModelPortali O05137.
SMRi O05137. Positions 2-722.
ModBasei Search...

2D gel databases

World-2DPAGE 0008:O05137.

Proteomic databases

PRIDEi O05137.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A functional glyoxylate bypass is mandatory for utilization of alkanes by Pseudomonas fluorescens."
    Artiguenave F.M., Delecu M., Vilagines R., Danglot C.
    Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: L6.5.

Entry informationi

Entry nameiMASZ_PSEFL
AccessioniPrimary (citable) accession number: O05137
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: February 19, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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