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O05137

- MASZ_PSEFL

UniProt

O05137 - MASZ_PSEFL

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Protein

Malate synthase G

Gene

glcB

Organism
Pseudomonas fluorescens
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei340 – 3401Proton acceptorUniRule annotation
Binding sitei340 – 3401GlyoxylateUniRule annotation
Metal bindingi432 – 4321MagnesiumUniRule annotation
Binding sitei432 – 4321GlyoxylateUniRule annotation
Metal bindingi460 – 4601MagnesiumUniRule annotation
Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei631 – 6311Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Synonyms:FC2.4
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Malate synthase GPRO_0000166892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei617 – 6171Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Proteomic databases

PRIDEiO05137.

2D gel databases

World-2DPAGE0008:O05137.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO05137.
SMRiO05137. Positions 2-722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni457 – 4604Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

O05137-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEHVQVGGL QVAKVLFDFV NNEAIPGTGI TADQFWAGAD KVIHDLAPKN
60 70 80 90 100
KALLAKRDDF QARIDTWHQT HAGQAHDPVA YKAFLQDIGY LLPEAADFQA
110 120 130 140 150
STQNVDDEIA RMAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDAISE
160 170 180 190 200
ADGAEKGKGY NKVRGDKVIA FARAFLDEAA PLSAGSHVDS TGYKIADGKL
210 220 230 240 250
IVSLKGGSNS GLRDDAQLIG FQGPAAQPIA ILLKHNGLHF EIQIDASTPV
260 270 280 290 300
GQTDAAGVKD VLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL
310 320 330 340 350
AEEVAKGGKT FTRTMNPDRV YTGVDGQDVT LHGRSLLFVR NVGHLMTIDA
360 370 380 390 400
ILDKAGNEVP EGILDGLLTS LAAIHSLNGN SSRKNSRTGS VYIVKPKMHG
410 420 430 440 450
PEEAAFTNEL FGRIEDVLNL PRNTLKVGIM DEERRTTVNL KACIKAASER
460 470 480 490 500
VVFINTGFLD RTGDEIHTSM EAGAMVRKAA MKTEKWIGAY ENWNVDIGLS
510 520 530 540 550
TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV PSPTAAALHA
560 570 580 590 600
LHYHKVDVFA RQAELAKRER ASVDDILTIP LAKNTDWSEE EIRNELDNNA
610 620 630 640 650
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ HIANWLRHGV
660 670 680 690 700
VTQDQVMESL KRMAPVVDRQ NAGDALYRPL APDFDSNIAF QAAVELVIEG
710 720
TKQPNGYTEP VLHRKRREFK AKNGL
Length:725
Mass (Da):78,861
Last modified:July 1, 1997 - v1
Checksum:i0C3326FE67C9B381
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11998 Genomic DNA. Translation: CAA72726.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11998 Genomic DNA. Translation: CAA72726.1 .

3D structure databases

ProteinModelPortali O05137.
SMRi O05137. Positions 2-722.
ModBasei Search...
MobiDBi Search...

2D gel databases

World-2DPAGE 0008:O05137.

Proteomic databases

PRIDEi O05137.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A functional glyoxylate bypass is mandatory for utilization of alkanes by Pseudomonas fluorescens."
    Artiguenave F.M., Delecu M., Vilagines R., Danglot C.
    Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: L6.5.

Entry informationi

Entry nameiMASZ_PSEFL
AccessioniPrimary (citable) accession number: O05137
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3