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O05118 (KPYK_METEA) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:pyk
Synonyms:pykA
Ordered Locus Names:MexAM1_META1p2941
OrganismMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) [Complete proteome] [HAMAP]
Taxonomic identifier272630 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence caution

The sequence AAB66498.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478Pyruvate kinase
PRO_0000112078

Sites

Metal binding371Potassium By similarity
Metal binding391Potassium By similarity
Metal binding691Potassium By similarity
Metal binding2191Magnesium By similarity
Metal binding2431Magnesium By similarity
Binding site351Substrate By similarity
Binding site2421Substrate; via amide nitrogen By similarity
Binding site2431Substrate; via amide nitrogen By similarity
Binding site2751Substrate By similarity
Site2171Transition state stabilizer By similarity

Experimental info

Sequence conflict601A → G in AAB66498. Ref.1
Sequence conflict1651V → A in AAB66498. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O05118 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: 6C58142F1B17E3AA

FASTA47851,801
        10         20         30         40         50         60 
MKRSRRTKIV ATLGPASDTP EMIEKLFHAG ADVFRINMSH LAREKLPERI EVIRTIEREA 

        70         80         90        100        110        120 
KRPIGILVDL QGPKLRLGTF VGDAAVLENG QTFVLDSDPT PGDTDRVFLP HPEILSALEP 

       130        140        150        160        170        180 
SHGILIDDGK LRLIVTEVSE GRAVTRVEVG GRISNRKGVS LPHTVLPVPA MTEKDRGDLE 

       190        200        210        220        230        240 
AGLAAGADWI AVSFVQRPED VAEVKKVAAG RALVMAKIEK PQALTRLDEI IEISDGIMVA 

       250        260        270        280        290        300 
RGDLGVEMPL EQVPGVQKRI TRVARRLGKP VVVATQMLES MITSPVPTRA EVSDVATAVY 

       310        320        330        340        350        360 
EGADAVMLSA ESAAGDFPVE AIGTMNRIAE QVERDALYWS ILMAQRSEPE PTASDAIAAA 

       370        380        390        400        410        420 
AHQIVEALSL RSIMAWTHSG STVLRLARAR PNASVIALTP KRETARRLTM AWGVHPIVTK 

       430        440        450        460        470 
DASDVDDMAF RAAKFAVRER FAEIGDRVII VAGVPFGIPG ATNMVRIAFV TREHAERA 

« Hide

References

« Hide 'large scale' references
[1]Chistoserdova L., Lidstrom M.E.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources."
Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E. expand/collapse author list , Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C., Lidstrom M.E.
PLoS ONE 4:E5584-E5584(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 14718 / DSM 1338 / AM1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U87316 Genomic DNA. Translation: AAB66498.1. Different initiation.
CP001510 Genomic DNA. Translation: ACS40693.1.
RefSeqYP_002963970.1. NC_012808.1.

3D structure databases

ProteinModelPortalO05118.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS40693; ACS40693; MexAM1_META1p2941.
GeneID7992827.
KEGGmea:Mex_1p2941.
PATRIC22511616. VBIMetExt101010_2874.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0469.
HOGENOMHOG000021558.
KOK00873.
OMANDETHAL.
OrthoDBEOG6GBMB0.
ProtClustDBCLSK2331328.

Enzyme and pathway databases

BioCycMEXT272630:GBY6-2787-MONOMER.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_METEA
AccessionPrimary (citable) accession number: O05118
Secondary accession number(s): C5AUT3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 22, 2009
Last modified: November 13, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways