Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate kinase

Gene

pyk

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk), Pyruvate kinase (pyk), Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35SubstrateBy similarity1
Metal bindingi37PotassiumBy similarity1
Metal bindingi39PotassiumBy similarity1
Metal bindingi69PotassiumBy similarity1
Sitei217Transition state stabilizerBy similarity1
Metal bindingi219MagnesiumBy similarity1
Binding sitei242Substrate; via amide nitrogenBy similarity1
Metal bindingi243MagnesiumBy similarity1
Binding sitei243Substrate; via amide nitrogenBy similarity1
Binding sitei275SubstrateBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Synonyms:pykA
Ordered Locus Names:MexAM1_META1p2941
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000009081 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120781 – 478Pyruvate kinaseAdd BLAST478

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p2941.

Structurei

3D structure databases

ProteinModelPortaliO05118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9. Bacteria.
COG0469. LUCA.
HOGENOMiHOG000021558.
KOiK00873.
OMAiTHEDHKE.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O05118-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSRRTKIV ATLGPASDTP EMIEKLFHAG ADVFRINMSH LAREKLPERI
60 70 80 90 100
EVIRTIEREA KRPIGILVDL QGPKLRLGTF VGDAAVLENG QTFVLDSDPT
110 120 130 140 150
PGDTDRVFLP HPEILSALEP SHGILIDDGK LRLIVTEVSE GRAVTRVEVG
160 170 180 190 200
GRISNRKGVS LPHTVLPVPA MTEKDRGDLE AGLAAGADWI AVSFVQRPED
210 220 230 240 250
VAEVKKVAAG RALVMAKIEK PQALTRLDEI IEISDGIMVA RGDLGVEMPL
260 270 280 290 300
EQVPGVQKRI TRVARRLGKP VVVATQMLES MITSPVPTRA EVSDVATAVY
310 320 330 340 350
EGADAVMLSA ESAAGDFPVE AIGTMNRIAE QVERDALYWS ILMAQRSEPE
360 370 380 390 400
PTASDAIAAA AHQIVEALSL RSIMAWTHSG STVLRLARAR PNASVIALTP
410 420 430 440 450
KRETARRLTM AWGVHPIVTK DASDVDDMAF RAAKFAVRER FAEIGDRVII
460 470
VAGVPFGIPG ATNMVRIAFV TREHAERA
Length:478
Mass (Da):51,801
Last modified:September 22, 2009 - v2
Checksum:i6C58142F1B17E3AA
GO

Sequence cautioni

The sequence AAB66498 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60A → G in AAB66498 (Ref. 1) Curated1
Sequence conflicti165V → A in AAB66498 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87316 Genomic DNA. Translation: AAB66498.1. Different initiation.
CP001510 Genomic DNA. Translation: ACS40693.1.
RefSeqiWP_003601864.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS40693; ACS40693; MexAM1_META1p2941.
KEGGimea:Mex_1p2941.
PATRICi22511616. VBIMetExt101010_2874.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87316 Genomic DNA. Translation: AAB66498.1. Different initiation.
CP001510 Genomic DNA. Translation: ACS40693.1.
RefSeqiWP_003601864.1. NC_012808.1.

3D structure databases

ProteinModelPortaliO05118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p2941.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS40693; ACS40693; MexAM1_META1p2941.
KEGGimea:Mex_1p2941.
PATRICi22511616. VBIMetExt101010_2874.

Phylogenomic databases

eggNOGiENOG4105CA9. Bacteria.
COG0469. LUCA.
HOGENOMiHOG000021558.
KOiK00873.
OMAiTHEDHKE.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPYK_METEA
AccessioniPrimary (citable) accession number: O05118
Secondary accession number(s): C5AUT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 22, 2009
Last modified: November 2, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.