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O05118

- KPYK_METEA

UniProt

O05118 - KPYK_METEA

Protein

Pyruvate kinase

Gene

pyk

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei35 – 351SubstrateBy similarity
    Metal bindingi37 – 371PotassiumBy similarity
    Metal bindingi39 – 391PotassiumBy similarity
    Metal bindingi69 – 691PotassiumBy similarity
    Sitei217 – 2171Transition state stabilizerBy similarity
    Metal bindingi219 – 2191MagnesiumBy similarity
    Binding sitei242 – 2421Substrate; via amide nitrogenBy similarity
    Metal bindingi243 – 2431MagnesiumBy similarity
    Binding sitei243 – 2431Substrate; via amide nitrogenBy similarity
    Binding sitei275 – 2751SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciMEXT272630:GBY6-2787-MONOMER.
    RETL1328306-WGS:GSTH-3610-MONOMER.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pyk
    Synonyms:pykA
    Ordered Locus Names:MexAM1_META1p2941
    OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
    Taxonomic identifieri272630 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
    ProteomesiUP000009081: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 478478Pyruvate kinasePRO_0000112078Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO05118.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021558.
    KOiK00873.
    OMAiYTSIRAS.
    OrthoDBiEOG6GBMB0.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O05118-1 [UniParc]FASTAAdd to Basket

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    MKRSRRTKIV ATLGPASDTP EMIEKLFHAG ADVFRINMSH LAREKLPERI    50
    EVIRTIEREA KRPIGILVDL QGPKLRLGTF VGDAAVLENG QTFVLDSDPT 100
    PGDTDRVFLP HPEILSALEP SHGILIDDGK LRLIVTEVSE GRAVTRVEVG 150
    GRISNRKGVS LPHTVLPVPA MTEKDRGDLE AGLAAGADWI AVSFVQRPED 200
    VAEVKKVAAG RALVMAKIEK PQALTRLDEI IEISDGIMVA RGDLGVEMPL 250
    EQVPGVQKRI TRVARRLGKP VVVATQMLES MITSPVPTRA EVSDVATAVY 300
    EGADAVMLSA ESAAGDFPVE AIGTMNRIAE QVERDALYWS ILMAQRSEPE 350
    PTASDAIAAA AHQIVEALSL RSIMAWTHSG STVLRLARAR PNASVIALTP 400
    KRETARRLTM AWGVHPIVTK DASDVDDMAF RAAKFAVRER FAEIGDRVII 450
    VAGVPFGIPG ATNMVRIAFV TREHAERA 478
    Length:478
    Mass (Da):51,801
    Last modified:September 22, 2009 - v2
    Checksum:i6C58142F1B17E3AA
    GO

    Sequence cautioni

    The sequence AAB66498.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti60 – 601A → G in AAB66498. 1 PublicationCurated
    Sequence conflicti165 – 1651V → A in AAB66498. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87316 Genomic DNA. Translation: AAB66498.1. Different initiation.
    CP001510 Genomic DNA. Translation: ACS40693.1.
    RefSeqiYP_002963970.1. NC_012808.1.

    Genome annotation databases

    EnsemblBacteriaiACS40693; ACS40693; MexAM1_META1p2941.
    GeneIDi7992827.
    KEGGimea:Mex_1p2941.
    PATRICi22511616. VBIMetExt101010_2874.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87316 Genomic DNA. Translation: AAB66498.1 . Different initiation.
    CP001510 Genomic DNA. Translation: ACS40693.1 .
    RefSeqi YP_002963970.1. NC_012808.1.

    3D structure databases

    ProteinModelPortali O05118.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS40693 ; ACS40693 ; MexAM1_META1p2941 .
    GeneIDi 7992827.
    KEGGi mea:Mex_1p2941.
    PATRICi 22511616. VBIMetExt101010_2874.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021558.
    KOi K00873.
    OMAi YTSIRAS.
    OrthoDBi EOG6GBMB0.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BioCyci MEXT272630:GBY6-2787-MONOMER.
    RETL1328306-WGS:GSTH-3610-MONOMER.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Chistoserdova L., Lidstrom M.E.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 14718 / DSM 1338 / AM1.

    Entry informationi

    Entry nameiKPYK_METEA
    AccessioniPrimary (citable) accession number: O05118
    Secondary accession number(s): C5AUT3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3