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O05118

- KPYK_METEA

UniProt

O05118 - KPYK_METEA

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Protein

Pyruvate kinase

Gene

pyk

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351SubstrateBy similarity
Metal bindingi37 – 371PotassiumBy similarity
Metal bindingi39 – 391PotassiumBy similarity
Metal bindingi69 – 691PotassiumBy similarity
Sitei217 – 2171Transition state stabilizerBy similarity
Metal bindingi219 – 2191MagnesiumBy similarity
Binding sitei242 – 2421Substrate; via amide nitrogenBy similarity
Metal bindingi243 – 2431MagnesiumBy similarity
Binding sitei243 – 2431Substrate; via amide nitrogenBy similarity
Binding sitei275 – 2751SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciMEXT272630:GBY6-2787-MONOMER.
RETL1328306-WGS:GSTH-3610-MONOMER.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Synonyms:pykA
Ordered Locus Names:MexAM1_META1p2941
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000009081: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478Pyruvate kinasePRO_0000112078Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO05118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021558.
KOiK00873.
OMAiYTSIRAS.
OrthoDBiEOG6GBMB0.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O05118-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRSRRTKIV ATLGPASDTP EMIEKLFHAG ADVFRINMSH LAREKLPERI
60 70 80 90 100
EVIRTIEREA KRPIGILVDL QGPKLRLGTF VGDAAVLENG QTFVLDSDPT
110 120 130 140 150
PGDTDRVFLP HPEILSALEP SHGILIDDGK LRLIVTEVSE GRAVTRVEVG
160 170 180 190 200
GRISNRKGVS LPHTVLPVPA MTEKDRGDLE AGLAAGADWI AVSFVQRPED
210 220 230 240 250
VAEVKKVAAG RALVMAKIEK PQALTRLDEI IEISDGIMVA RGDLGVEMPL
260 270 280 290 300
EQVPGVQKRI TRVARRLGKP VVVATQMLES MITSPVPTRA EVSDVATAVY
310 320 330 340 350
EGADAVMLSA ESAAGDFPVE AIGTMNRIAE QVERDALYWS ILMAQRSEPE
360 370 380 390 400
PTASDAIAAA AHQIVEALSL RSIMAWTHSG STVLRLARAR PNASVIALTP
410 420 430 440 450
KRETARRLTM AWGVHPIVTK DASDVDDMAF RAAKFAVRER FAEIGDRVII
460 470
VAGVPFGIPG ATNMVRIAFV TREHAERA
Length:478
Mass (Da):51,801
Last modified:September 22, 2009 - v2
Checksum:i6C58142F1B17E3AA
GO

Sequence cautioni

The sequence AAB66498.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601A → G in AAB66498. 1 PublicationCurated
Sequence conflicti165 – 1651V → A in AAB66498. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87316 Genomic DNA. Translation: AAB66498.1. Different initiation.
CP001510 Genomic DNA. Translation: ACS40693.1.
RefSeqiYP_002963970.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS40693; ACS40693; MexAM1_META1p2941.
GeneIDi7992827.
KEGGimea:Mex_1p2941.
PATRICi22511616. VBIMetExt101010_2874.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87316 Genomic DNA. Translation: AAB66498.1 . Different initiation.
CP001510 Genomic DNA. Translation: ACS40693.1 .
RefSeqi YP_002963970.1. NC_012808.1.

3D structure databases

ProteinModelPortali O05118.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS40693 ; ACS40693 ; MexAM1_META1p2941 .
GeneIDi 7992827.
KEGGi mea:Mex_1p2941.
PATRICi 22511616. VBIMetExt101010_2874.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021558.
KOi K00873.
OMAi YTSIRAS.
OrthoDBi EOG6GBMB0.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
BioCyci MEXT272630:GBY6-2787-MONOMER.
RETL1328306-WGS:GSTH-3610-MONOMER.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Chistoserdova L., Lidstrom M.E.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14718 / DSM 1338 / AM1.

Entry informationi

Entry nameiKPYK_METEA
AccessioniPrimary (citable) accession number: O05118
Secondary accession number(s): C5AUT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 22, 2009
Last modified: November 26, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3