Reviewed, UniProtKB/Swiss-Prot O05100 (DEF1_CLOAB)

Names and origin

Protein names

Recommended name:
    Peptide deformylase 1
      Short name=PDF 1
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase 1

Gene names

Name:	def1
Ordered Locus Names:	CA_C1722

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity.
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 150	150	Peptide deformylase 1 HAMAP MF_00163		PRO_0000082766
Sites
	Active site	131	1	By similarity
	Metal binding	88	1	Iron By similarity
	Metal binding	130	1	Iron By similarity
	Metal binding	134	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	O05100-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 07760E6A5CB2606D	FASTA	150	16,617
	10 20 30 40 50 60 MAIRSIRKYG DELLRKKSRK VEKIDKRLLT LIDDMFETMY NADGVGLAAP QVGILKRLVV 70 80 90 100 110 120 IDVGEGPVVL INPEILETSG KAVDVEGCLS IPERQGEVER PTYVKAKALN EKGEEIVIEA 130 140 150 EDLFARAICH ETDHLNGVLF VDKLAESEGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complementation of an Escherichia coli polypeptide deformylase mutant with a gene from Clostridium acetobutylicum ATCC 824." Belouski E., Gui L., Rudolph F.B., Bennett G.N. Curr. Microbiol. 36:248-249(1998) [PubMed: 9504995] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U52368 Genomic DNA. Translation: AAB50347.1. AE001437 Genomic DNA. Translation: AAK79688.1.
PIR	E97112.
RefSeq	NP_348348.1.
3D structure databases
HSSP	HSSP built from PDB template 1LME based on UniProtKB P96113.
ModBase	Search...
Genome annotation databases
GeneID	1117905.
GenomeReviews	Gene locus CA_C1722 in contig AE001437_GR.
KEGG	cac:CAC1722.
NMPDR	fig|272562.1.peg.1877.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O05100.
OMA	IIMHEND.
Enzyme and pathway databases
BioCyc	CACE272562:CAC1722-MON.
BRENDA	3.5.1.88. 2866.
Family and domain databases
HAMAP	MF_00163. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
ProDom	PD003844. Fmet_deformylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF1_CLOAB

Accession

Primary (citable) accession number: O05100

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)