O05052 (T1RH_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative type I restriction enzyme HindVIIP R protein
EC=3.1.21.3

Gene names

Ordered Locus Names:

HI_1285

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	1055 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Subunit R is required for both nuclease and ATPase activities, but not for modification By similarity.
Catalytic activity	Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.
Subunit structure	The type I restriction/modification system is composed of three polypeptides R, M and S By similarity.
Sequence similarities	Belongs to the HsdR family. Contains 1 helicase ATP-binding domain.

Ontologies

Keywords
Biological process	Restriction system
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW Type I site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1055	1055	Putative type I restriction enzyme HindVIIP R protein		PRO_0000077262
Regions
	Domain	287 – 468	182	Helicase ATP-binding

Sequences

	Sequence		Length	Mass (Da)
	O05052 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: C5BEF298E7E05C67	FASTA	1,055	120,710
	10 20 30 40 50 60 MLNENDIEQL TLQRLQSLGW EYRYGKDLPV HEGKFARGDL SGVVFVEQLR EAVRKLNPQL 70 80 90 100 110 120 PESAVDSVVK SATKSDIGDL VVRNQTFYKL LRDGVRVEYT QNGEQKIEMV RLVDFEHWGN 130 140 150 160 170 180 NRFVAVNQLE IRSRKGGKRI PDIIGFVNGL PLVVFELKNP LRESADLLQA FNQFETYKDE 190 200 210 220 230 240 IAELFVYNQA LIISDGIVAR LGSLSADFQR FTPWKVVDEK NKSARLYFDD ELQSLLNGLM 250 260 270 280 290 300 QPEDLLDYIR YFVLFERDSV GKTIKKIAAY HQYYGVNEAV DSTIWATSEK GDRRIGVMWH 310 320 330 340 350 360 TQGSGKSISM LFYAGKLLAQ PELKNPTIVV VTDRNDLDGQ LFQTFSSGKD LIKQTPQQVE 370 380 390 400 410 420 DRDQLRQLLA QNEVGGVFFT TIQKFALNEE ESRFPILNER NNIIVISDEA HRSQYGFTQK 430 440 450 460 470 480 LHNGKFQTGY ARHLRDALPN ASFIGFTGTP ISLEDKDTQD VFGRYVSIYD LQDAVEDGAT 490 500 510 520 530 540 VPIVYDDARQ IRLNKKDHDA LFAEIDALLE EEPSTSLRLR EKLLGSQERL IELAADFVQH 550 560 570 580 590 600 FAKRNEVVDS KAMMVVSSRQ ICVDLYNQII ALHPEWHSDN INEGAIKIVM TGSASDTPEM 610 620 630 640 650 660 QKHIYSKQEK QTLERRFKDP NDPLKVVIVR DMWLTGFDAP CCNTMYLDKP MKGHNLMQAI 670 680 690 700 710 720 ARVNRVFANK SRENGGLIVD YVGLAKELRA ATQQYTNSTG KGQLAEDVQS VFFKMKEQLE 730 740 750 760 770 780 FIRTLFATPI EGKTFDVQAA LEKDNPNDLL MAIRFAANHI LSLDQLSFDG KAHEQHWFNK 790 800 810 820 830 840 KETEPRKKAF LKTAGLVKKG YMLCGTLAEV EPYNQEIAFY DAVRAILTKR EQKGTGTNER 850 860 870 880 890 900 QILLKKLVNQ TVYSEGVIDL FDLLEKPQPQ ISLLSEEFLQ TVKNSPTKNL WVSAMERYLA 910 920 930 940 950 960 SEIKVKSGTN LTLQKDFERR LKEALNQYHN HNLTVVEILD ELFKMSQDFQ ERLALGKKLG 970 980 990 1000 1010 1020 LTKEELAFYE ALSQNQSAKD LMGDEVLSKL AKEITETLRK SVTIDWQYKE AVRARIRLLV 1030 1040 1050 RRALQKYKYP PDKQEEAVTY VIKQAEEIAE DLTGL

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22934.1.
PIR	F64114.
RefSeq	NP_439437.1. NC_000907.1.
3D structure databases
ProteinModelPortal	O05052.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI1285.
Protein family/group databases
REBASE	1149. HindI.
Protocols and materials databases
DNASU	950228.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22934; AAC22934; HI_1285.
GeneID	950228.
KEGG	hin:HI1285.
PATRIC	20191253. VBIHaeInf48452_1337.
Phylogenomic databases
eggNOG	COG0610.
KO	K01153.
OMA	LITRDDR.
ProtClustDB	CLSK808504.
Family and domain databases
InterPro	IPR021810. DUF3387. IPR006935. Helicase/UvrB_dom. IPR014001. Helicase_ATP-bd. IPR007409. Restrct_endonuc_type1_HsdR_N. IPR004473. Restrct_endonuc_typeI_HsdR. [Graphical view]
Pfam	PF11867. DUF3387. 1 hit. PF04313. HSDR_N. 1 hit. PF04851. ResIII. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00348. hsdR. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T1RH_HAEIN

Accession

Primary (citable) accession number: O05052

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program