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Protein

Na(+)-translocating NADH-quinone reductase subunit F

Gene

nqrF

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na+ ions from the cytoplasm to the periplasm. The first step is catalyzed by NqrF, which accepts electrons from NADH and reduces ubiquinone-1 to ubisemiquinone by a one-electron transfer pathway.UniRule annotation

Catalytic activityi

NADH + ubiquinone + n Na+(In) = NAD+ + ubiquinol + n Na+(Out).UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster.UniRule annotation
  • FADUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi73Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi79Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi82Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi114Iron-sulfur (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD, Sodium, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)-translocating NADH-quinone reductase subunit FUniRule annotation (EC:1.6.5.8UniRule annotation)
Short name:
Na(+)-NQR subunit FUniRule annotation
Short name:
Na(+)-translocating NQR subunit FUniRule annotation
Alternative name(s):
NQR complex subunit FUniRule annotation
NQR-1 subunit FUniRule annotation
Gene namesi
Name:nqrFUniRule annotation
Ordered Locus Names:HI_0171
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei5 – 25HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000744961 – 411Na(+)-translocating NADH-quinone reductase subunit FAdd BLAST411

Interactioni

Subunit structurei

Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF.UniRule annotation

Protein-protein interaction databases

STRINGi71421.HI0171.

Structurei

3D structure databases

ProteinModelPortaliO05012.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 1302Fe-2S ferredoxin-typeUniRule annotationAdd BLAST95
Domaini133 – 273FAD-binding FR-typeUniRule annotationAdd BLAST141

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni276 – 393CatalyticAdd BLAST118

Sequence similaritiesi

Belongs to the NqrF family.UniRule annotation
Contains 1 2Fe-2S ferredoxin-type domain.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D26. Bacteria.
COG2871. LUCA.
KOiK00351.
OMAiEEHGIIM.
PhylomeDBiO05012.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR010205. NqrF.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
PRINTSiPR00371. FPNCR.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O05012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSVILALG IAAFTVIVLV LVAIILFAKS KLVDSGDITI DINDDPEKAI
60 70 80 90 100
TLPAGGKLLG ALASKGIFVS SACGGGGSCG QCIVKVKNGG GEILPTELSH
110 120 130 140 150
INKREAKEGY RLACQVNVKG NMEVELPEEI FGVKKWECTV ISNDNKATFI
160 170 180 190 200
KELKLAIPEG EEVPFRAGGY IQIEAEPHVV NYKDFDIPEE YHEDWDKYDL
210 220 230 240 250
WRYVSKVDEH IIRAYSMASY PEEKGIIMLN VRIATPPPRQ PDAPPGQMSS
260 270 280 290 300
YIWSLKAGDK VTISGPFGEF FAKETDAEMV FIGGGAGMAP MRSHIFDQLK
310 320 330 340 350
RLHSKRKMSF WYGARSKREI FYQEDFDQLQ AENPNFVWHV ALSDALPEDN
360 370 380 390 400
WTGYTGFIHN VLYENYLKNH EAPEDCEYYM CGPPVMNAAV IKMLKDLGVE
410
DENILLDDFG G
Length:411
Mass (Da):45,705
Last modified:July 1, 1997 - v1
Checksum:iED560053E66D23E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21841.1.
PIRiD64052.
RefSeqiNP_438339.1. NC_000907.1.
WP_005648666.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21841; AAC21841; HI_0171.
GeneIDi951079.
KEGGihin:HI0171.
PATRICi20188837. VBIHaeInf48452_0175.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21841.1.
PIRiD64052.
RefSeqiNP_438339.1. NC_000907.1.
WP_005648666.1. NC_000907.1.

3D structure databases

ProteinModelPortaliO05012.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0171.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21841; AAC21841; HI_0171.
GeneIDi951079.
KEGGihin:HI0171.
PATRICi20188837. VBIHaeInf48452_0175.

Phylogenomic databases

eggNOGiENOG4105D26. Bacteria.
COG2871. LUCA.
KOiK00351.
OMAiEEHGIIM.
PhylomeDBiO05012.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR010205. NqrF.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
PRINTSiPR00371. FPNCR.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNQRF_HAEIN
AccessioniPrimary (citable) accession number: O05012
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: July 1, 1997
Last modified: October 5, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.