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Protein

Biotin carboxylase, chloroplastic

Gene

CAC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.1 Publication

Catalytic activityi

ATP + biotin-[carboxyl-carrier-protein] + HCO3- = ADP + phosphate + carboxy-biotin-[carboxyl-carrier-protein].1 Publication
ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Kineticsi

  1. KM=2.3 mM for biotin1 Publication
  2. KM=88 mM for bicarbonate1 Publication
  1. Vmax=16 nmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.3-8.9.1 Publication

Pathwayi: malonyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes malonyl-CoA from acetyl-CoA.
Proteins known to be involved in this subpathway in this organism are:
  1. Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha, chloroplastic (CAC3), Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic (accD), Biotin carboxylase, chloroplastic (CAC2), Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic (accD), Acetyl-CoA carboxylase 2 (ACC2), Acetyl-CoA carboxylase 1 (ACC1)
This subpathway is part of the pathway malonyl-CoA biosynthesis, which is itself part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes malonyl-CoA from acetyl-CoA, the pathway malonyl-CoA biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei188ATPBy similarity1
Binding sitei272ATPBy similarity1
Binding sitei307ATPBy similarity1
Metal bindingi347Magnesium or manganese 1PROSITE-ProRule annotation1
Metal bindingi360Magnesium or manganese 1PROSITE-ProRule annotation1
Metal bindingi360Magnesium or manganese 2PROSITE-ProRule annotation1
Metal bindingi362Magnesium or manganese 2PROSITE-ProRule annotation1
Active sitei364By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi220 – 281ATPPROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

ATP-binding, Biotin, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G35360-MONOMER.
UniPathwayiUPA00655; UER00711.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin carboxylase, chloroplastic (EC:6.3.4.141 Publication)
Alternative name(s):
Acetyl-CoA carboxylase subunit A (EC:6.4.1.21 Publication)
Short name:
ACC
Gene namesi
Name:CAC2
Ordered Locus Names:At5g35360
ORF Names:T26D22.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G35360.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 70ChloroplastSequence analysisAdd BLAST70
ChainiPRO_000039177271 – 537Biotin carboxylase, chloroplasticAdd BLAST467

Proteomic databases

PaxDbiO04983.
PRIDEiO04983.

Expressioni

Tissue specificityi

Accumulates in fatty acids synthesizing tissues. Mostly expressed in siliques, developing leaves, and flowers, present in roots and embryos (especially at torpedo stage), and, to a lower extent, in mature leaves.3 Publications

Gene expression databases

ExpressionAtlasiO04983. baseline and differential.
GenevisibleiO04983. AT.

Interactioni

Subunit structurei

Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein, biotin carboxylase and two subunits each of ACCase subunit alpha and ACCase plastid-coded subunit beta (accD).Curated

Protein-protein interaction databases

BioGridi18751. 5 interactors.
IntActiO04983. 1 interactor.
STRINGi3702.AT5G35360.3.

Structurei

3D structure databases

ProteinModelPortaliO04983.
SMRiO04983.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini192 – 389ATP-graspPROSITE-ProRule annotationAdd BLAST198

Sequence similaritiesi

Contains 1 ATP-grasp domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IU5C. Eukaryota.
COG0439. LUCA.
HOGENOMiHOG000008988.
InParanoidiO04983.
KOiK01961.
PhylomeDBiO04983.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR004549. Acetyl_CoA_COase_biotin_COase.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005481. BC-like_N.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02785. Biotin_carb_C. 1 hit.
PF00289. Biotin_carb_N. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00514. accC. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O04983-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDASMITNSK SITSPPSLAL GKSGGGGVIR SSLCNLMMPS KVNFPRQRTQ
60 70 80 90 100
TLKVSQKKLK RATSGGLGVT CSGGDKILVA NRGEIAVRVI RTAHEMGIPC
110 120 130 140 150
VAVYSTIDKD ALHVKLADEA VCIGEAPSNQ SYLVIPNVLS AAISRGCTML
160 170 180 190 200
HPGYGFLSEN ALFVEMCRDH GINFIGPNPD SIRVMGDKAT ARETMKNAGV
210 220 230 240 250
PTVPGSDGLL QSTEEAVRVA NEIGFPVMIK ATAGGGGRGM RLAKEPGEFV
260 270 280 290 300
KLLQQAKSEA AAAFGNDGCY LEKFVQNPRH IEFQVLADKF GNVVHFGERD
310 320 330 340 350
CSIQRRNQKL LEEAPSPALT AELRKAMGDA AVAAAASIGY IGVGTVEFLL
360 370 380 390 400
DERGSFYFME MNTRIQVEHP VTEMIYSVDL IEEQIRVAMG EKLRYKQEDI
410 420 430 440 450
VLRGHSIECR INAEDPFKGF RPGPGRITSY LPSGGPFVRM DSHVYSDYVV
460 470 480 490 500
PPSYDSLLGK LIVWAPTREK AIERMKRALN DTIITGVPTT INYHKLILDV
510 520 530
EDFKNGKVDT AFIVKHEEEL AEPQEIVAVK DLTNATV
Length:537
Mass (Da):58,387
Last modified:July 1, 1997 - v1
Checksum:iCB57C973A2A735C8
GO

Sequence cautioni

The sequence CAA70282 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181S → T in CAA70282 (PubMed:9349276).Curated1
Sequence conflicti230K → T in CAA70282 (PubMed:9349276).Curated1
Sequence conflicti314Missing in CAA70282 (PubMed:9349276).Curated1
Sequence conflicti447D → N in AAK25989 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09061 Genomic DNA. Translation: CAA70282.1. Sequence problems.
U90879 mRNA. Translation: AAC09008.1.
U91414 Genomic DNA. Translation: AAC09009.1.
AB025636 Genomic DNA. Translation: BAB11486.1.
AF058826 Genomic DNA. Translation: AAC13611.1.
CP002688 Genomic DNA. Translation: AED93956.1.
AF360279 mRNA. Translation: AAK25989.1.
AY142630 mRNA. Translation: AAN13088.1.
AY085968 mRNA. Translation: AAM63178.1.
PIRiT01180.
RefSeqiNP_198386.1. NM_122927.4. [O04983-1]
UniGeneiAt.22848.
At.70066.

Genome annotation databases

EnsemblPlantsiAT5G35360.1; AT5G35360.1; AT5G35360. [O04983-1]
GeneIDi833497.
GrameneiAT5G35360.1; AT5G35360.1; AT5G35360.
KEGGiath:AT5G35360.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09061 Genomic DNA. Translation: CAA70282.1. Sequence problems.
U90879 mRNA. Translation: AAC09008.1.
U91414 Genomic DNA. Translation: AAC09009.1.
AB025636 Genomic DNA. Translation: BAB11486.1.
AF058826 Genomic DNA. Translation: AAC13611.1.
CP002688 Genomic DNA. Translation: AED93956.1.
AF360279 mRNA. Translation: AAK25989.1.
AY142630 mRNA. Translation: AAN13088.1.
AY085968 mRNA. Translation: AAM63178.1.
PIRiT01180.
RefSeqiNP_198386.1. NM_122927.4. [O04983-1]
UniGeneiAt.22848.
At.70066.

3D structure databases

ProteinModelPortaliO04983.
SMRiO04983.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi18751. 5 interactors.
IntActiO04983. 1 interactor.
STRINGi3702.AT5G35360.3.

Proteomic databases

PaxDbiO04983.
PRIDEiO04983.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G35360.1; AT5G35360.1; AT5G35360. [O04983-1]
GeneIDi833497.
GrameneiAT5G35360.1; AT5G35360.1; AT5G35360.
KEGGiath:AT5G35360.

Organism-specific databases

TAIRiAT5G35360.

Phylogenomic databases

eggNOGiENOG410IU5C. Eukaryota.
COG0439. LUCA.
HOGENOMiHOG000008988.
InParanoidiO04983.
KOiK01961.
PhylomeDBiO04983.

Enzyme and pathway databases

UniPathwayiUPA00655; UER00711.
BioCyciARA:AT5G35360-MONOMER.

Miscellaneous databases

PROiO04983.

Gene expression databases

ExpressionAtlasiO04983. baseline and differential.
GenevisibleiO04983. AT.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR004549. Acetyl_CoA_COase_biotin_COase.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005481. BC-like_N.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02785. Biotin_carb_C. 1 hit.
PF00289. Biotin_carb_N. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00514. accC. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACCC_ARATH
AccessioniPrimary (citable) accession number: O04983
Secondary accession number(s): P93650, Q9C5F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.