O04977 (FENR1_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferredoxin--NADP reductase, leaf-type isozyme, chloroplastic
Short name=FNR
EC=1.18.1.2

Gene names

Name:

PETH

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.
Catalytic activity	2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.
Cofactor	FAD.
Pathway	Energy metabolism; photosynthesis.
Subcellular location	Plastid › chloroplast stroma. Plastid › chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side Probable. Note: In the vicinity of the photosystem I in the non-stacked and fringe portion of the membrane.
Sequence similarities	Belongs to the ferredoxin--NADP reductase type 1 family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro ferredoxin-NADP+ reductase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 62	62	Chloroplast Potential
	Chain	63 – 362	300	Ferredoxin--NADP reductase, leaf-type isozyme, chloroplastic		PRO_0000019413
Regions
	Domain	83 – 205	123	FAD-binding FR-type
	Nucleotide binding	141 – 144	4	FAD By similarity
	Nucleotide binding	162 – 164	3	FAD By similarity
	Nucleotide binding	179 – 181	3	FAD By similarity
	Nucleotide binding	252 – 253	2	NADP By similarity
	Nucleotide binding	282 – 283	2	NADP By similarity
	Nucleotide binding	321 – 322	2	NADP By similarity
Sites
	Binding site	144	1	NADP By similarity
	Binding site	164	1	NADP By similarity
	Binding site	168	1	FAD By similarity
	Binding site	220	1	FAD By similarity
	Binding site	220	1	NADP; via amide nitrogen By similarity
	Binding site	292	1	NADP By similarity
	Binding site	360	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O04977 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 338EB053B3EB018A	FASTA	362	40,445
	10 20 30 40 50 60 MATAVSAAVS LPSSKSTSFS SRTSIISTDK INFNKVPLYY RNVSGGSRLV SIRAQVTTEA 70 80 90 100 110 120 PAKVEKISKK QDEGVIVNKF RPKEPYVGRC LLNTKITGDD APGETWHMVF STEGEVPYRE 130 140 150 160 170 180 GQSIGVIADG VDANGKPHKL RLYSTASSAL GDFGDSKTVS LCVKRLVYTN DKGEEVKGVC 190 200 210 220 230 240 SNFLCDLKPG AEVKITGPVG KEMLMPKDPN ATVIMLATGT GIAPFRSFLW KMFFEKHEDY 250 260 270 280 290 300 KFNGTAWLFL GVPTSSSLLY KEEFEKMKEK APENFRLDFA VSREQTNEKG EKMYIQTRMA 310 320 330 340 350 360 QYAEELWTLL QKDNTFIYMC GLKGMEQGID EIMSALAERD GIVWADYKKQ LKKAEQWNVE VY

References

[1]	Hajirezaei M., Krause K.P., Sonnewald U. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Samsun NN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y14032 mRNA. Translation: CAA74359.1.
PIR	T04079.
3D structure databases
ProteinModelPortal	O04977.
SMR	O04977. Positions 67-362.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR015701. Fd_Red. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR012146. FNR. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
PANTHER	PTHR19384:SF1. FRD_Red. 1 hit.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF000361. Frd-NADP+_RD. 1 hit.
PRINTS	PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FENR1_TOBAC

Accession

Primary (citable) accession number: O04977

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program