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Protein

Alpha-amylase

Gene

amy

Organism
Hordeum vulgare (Barley)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei203 – 2031NucleophileUniRule annotation
Active sitei228 – 2281Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotationImported, Hydrolase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Gene namesi
Name:amyImported
OrganismiHordeum vulgare (Barley)Imported
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Pathology & Biotechi

Protein family/group databases

Allergomei419. Hor v 16.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 437414Alpha-amylaseSequence analysisPRO_5004156762Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi4513.MLOC_56170.1.

Structurei

3D structure databases

ProteinModelPortaliO04964.
SMRiO04964. Positions 24-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 366342AamyInterPro annotationAdd
BLAST
Domaini367 – 42761Alpha-amyl_C2InterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

O04964-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKSHICCF SLLLLLFAGL ASGHQVLFQG FNWESWKQSG GWYNMMLGKV
60 70 80 90 100
DDIAAAGVTH VWLPPPSHSV AAEGYMPGRL YDIDASKYGN AAELKSLIGA
110 120 130 140 150
LHGNGVQAIA DIVINHRCAD YKDSRGIYCI YEGGTSDSRL DWGPHMICRD
160 170 180 190 200
DTKYSDGTAN LDTGADYADA PDIDHLNHRV QSELKEWLLW LKSDLGFDAW
210 220 230 240 250
RLDFARGYSA EMAKVYIDGT SPSLAVAEIW DGMVPGGDGK PNYDQDAHRQ
260 270 280 290 300
NLVNWVDKVG GAASAGMVFD FTTKGILNVA VEGELWRLID PQGKAPGVMG
310 320 330 340 350
WWPAKAVTFI DNHDTGSTQA KWPFPSDKIM QGYAYILTHS GIPCIFYDHF
360 370 380 390 400
FNLGFKDEIA ALVAIRKRNG ITATSALKIL MHDGDAYVAE IDSKVVVKIG
410 420 430
SRYDVGAVIP AGFVTSAHGK DYAVWEKIAA AATLQRS
Length:437
Mass (Da):47,776
Last modified:July 1, 1997 - v1
Checksum:iE163524C88CB2480
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11276 mRNA. Translation: CAA72143.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11276 mRNA. Translation: CAA72143.1.

3D structure databases

ProteinModelPortaliO04964.
SMRiO04964. Positions 24-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4513.MLOC_56170.1.

Protein family/group databases

Allergomei419. Hor v 16.
CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiO04964_HORVU
AccessioniPrimary (citable) accession number: O04964
Entry historyi
Integrated into UniProtKB/TrEMBL: July 1, 1997
Last sequence update: July 1, 1997
Last modified: May 11, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.