Reviewed, UniProtKB/Swiss-Prot O04955 (GSHRC_BRARP)

Names and origin

Protein names

Recommended name:
    Glutathione reductase, cytosolic
      Short name=GRase
      Short name=GR
    EC=1.8.1.7

Gene names

Name:

GR1

Organism

Brassica rapa subsp. pekinensis (Chinese cabbage)

Taxonomic identifier

51351 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol By similarity.
Catalytic activity	2 glutathione + NADP+ = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Induction	By paraquat and ozone. Ref.1
Miscellaneous	The active site is a redox-active disulfide bond By similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Glutathione reductase, cytosolic		PRO_0000067962
Regions
	Nucleotide binding	67 – 76	10	FAD By similarity
Sites
	Active site	475	1	Proton acceptor By similarity
	Binding site	237	1	NADP By similarity
	Binding site	243	1	NADP By similarity
Amino acid modifications
	Disulfide bond	76 ↔ 81		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	O04955-1 [UniParc]. Last modified October 1, 2000. Version 2. Checksum: 397D978EE114B6BE	FASTA	502	54,059
	10 20 30 40 50 60 MARKMLSDGE LNKAAAAGEE ATTETHYDFD LFVIGAGSGG VRAARFSANN GAKVGICELP 70 80 90 100 110 120 FHPISSEEIG GVGGTCVIRG CVPKKILVYG ATYGGELEDA RNYGWEINGN VDFNWKKLLQ 130 140 150 160 170 180 KKTDEILRLN NIYKRLLANA AVKLYEGEGR IVGPNEVEVR QIDGTKISYT AKHILIATGS 190 200 210 220 230 240 RAQKPNIPGH ELAITSDEAL SLEEFPKRAI VLGGGYIAVE FASIWRGMGA TVDLFFRKEL 250 260 270 280 290 300 PLRGFDDEMR ALVARNLEGR GINLHPQTSL AELIKTDDGI KVISSHGEEF VADVVLFATG 310 320 330 340 350 360 RIPNTKRLNL EAVGVELDQA GAVKVDEYSR TNIPSIWAVG DATNRINLTP VALMEATCFA 370 380 390 400 410 420 NTVFGGKPAK ADYTNVACAV FCIPPLAVVG LSEEEAVEKA TGDILVFTSG FNPMKNTISG 430 440 450 460 470 480 RQEKSLMKLI VDEKTDKVIG ASMCGPDAAE IMQGIAIALK CGATKAQFDS TVGIHPSSAE 490 500 EFVTMRTVTR RIAYKAKPQT SL

References

[1]	"Molecular cloning and characterization of the gene encoding glutathione reductase in Brassica campestris." Lee H.S., Jo J.K., Son D.Y. Biochim. Biophys. Acta 1395:309-314(1998) [PubMed: 9512665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Strain: cv. Seoul. Tissue: Leaf.
[2]	Lee H.S., Chung M.S., Jo J.K., Son D.Y. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Genomic cloning and its expression analysis of glutathione reductase from Brassica campestris var. Pekinensis." Lee H.S., Lee B., Won S., Jo J.K. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Seoul.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF008441 mRNA. Translation: AAC49980.2. AF255651 Genomic DNA. Translation: AAF67753.1.
PIR	T14394.
3D structure databases
HSSP	HSSP built from PDB template 1ONF based on UniProtKB Q94655.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.8.1.7. 142183.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006324. Glut_reduct_pln. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01424. gluta_reduc_2. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHRC_BRARP

Accession

Primary (citable) accession number: O04955

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	October 1, 2000
Last modified:	September 22, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)