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O04955

- GSHRC_BRARP

UniProt

O04955 - GSHRC_BRARP

Protein

Glutathione reductase, cytosolic

Gene

GR1

Organism
Brassica rapa subsp. pekinensis (Chinese cabbage) (Brassica pekinensis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Maintains high levels of reduced glutathione in the cytosol.By similarity

    Catalytic activityi

    2 glutathione + NADP+ = glutathione disulfide + NADPH.

    Cofactori

    Binds 1 FAD per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei237 – 2371NADPBy similarity
    Binding sitei243 – 2431NADPBy similarity
    Active sitei475 – 4751Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi67 – 7610FADBy similarity

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. glutathione-disulfide reductase activity Source: UniProtKB-EC
    3. NADP binding Source: InterPro

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro
    2. glutathione metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione reductase, cytosolic (EC:1.8.1.7)
    Short name:
    GR
    Short name:
    GRase
    Gene namesi
    Name:GR1
    OrganismiBrassica rapa subsp. pekinensis (Chinese cabbage) (Brassica pekinensis)
    Taxonomic identifieri51351 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica
    ProteomesiUP000011750: Unplaced

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502Glutathione reductase, cytosolicPRO_0000067962Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi76 ↔ 81Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Inductioni

    By paraquat and ozone.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO04955.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Redox-active center

    Family and domain databases

    Gene3Di3.30.390.30. 1 hit.
    InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR006324. Glut-diS_reduct.
    IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR012999. Pyr_OxRdtase_I_AS.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view]
    PfamiPF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    PF02852. Pyr_redox_dim. 1 hit.
    [Graphical view]
    PRINTSiPR00368. FADPNR.
    SUPFAMiSSF55424. SSF55424. 1 hit.
    TIGRFAMsiTIGR01424. gluta_reduc_2. 1 hit.
    PROSITEiPS00076. PYRIDINE_REDOX_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O04955-1 [UniParc]FASTAAdd to Basket

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    MARKMLSDGE LNKAAAAGEE ATTETHYDFD LFVIGAGSGG VRAARFSANN    50
    GAKVGICELP FHPISSEEIG GVGGTCVIRG CVPKKILVYG ATYGGELEDA 100
    RNYGWEINGN VDFNWKKLLQ KKTDEILRLN NIYKRLLANA AVKLYEGEGR 150
    IVGPNEVEVR QIDGTKISYT AKHILIATGS RAQKPNIPGH ELAITSDEAL 200
    SLEEFPKRAI VLGGGYIAVE FASIWRGMGA TVDLFFRKEL PLRGFDDEMR 250
    ALVARNLEGR GINLHPQTSL AELIKTDDGI KVISSHGEEF VADVVLFATG 300
    RIPNTKRLNL EAVGVELDQA GAVKVDEYSR TNIPSIWAVG DATNRINLTP 350
    VALMEATCFA NTVFGGKPAK ADYTNVACAV FCIPPLAVVG LSEEEAVEKA 400
    TGDILVFTSG FNPMKNTISG RQEKSLMKLI VDEKTDKVIG ASMCGPDAAE 450
    IMQGIAIALK CGATKAQFDS TVGIHPSSAE EFVTMRTVTR RIAYKAKPQT 500
    SL 502
    Length:502
    Mass (Da):54,059
    Last modified:October 1, 2000 - v2
    Checksum:i397D978EE114B6BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF008441 mRNA. Translation: AAC49980.2.
    AF255651 Genomic DNA. Translation: AAF67753.1.
    PIRiT14394.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF008441 mRNA. Translation: AAC49980.2 .
    AF255651 Genomic DNA. Translation: AAF67753.1 .
    PIRi T14394.

    3D structure databases

    ProteinModelPortali O04955.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.390.30. 1 hit.
    InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR006324. Glut-diS_reduct.
    IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR012999. Pyr_OxRdtase_I_AS.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view ]
    Pfami PF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    PF02852. Pyr_redox_dim. 1 hit.
    [Graphical view ]
    PRINTSi PR00368. FADPNR.
    SUPFAMi SSF55424. SSF55424. 1 hit.
    TIGRFAMsi TIGR01424. gluta_reduc_2. 1 hit.
    PROSITEi PS00076. PYRIDINE_REDOX_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of the gene encoding glutathione reductase in Brassica campestris."
      Lee H.S., Jo J.K., Son D.Y.
      Biochim. Biophys. Acta 1395:309-314(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
      Strain: cv. Seoul.
      Tissue: Leaf.
    2. Lee H.S., Chung M.S., Jo J.K., Son D.Y.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Genomic cloning and its expression analysis of glutathione reductase from Brassica campestris var. Pekinensis."
      Lee H.S., Lee B., Won S., Jo J.K.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: cv. Seoul.

    Entry informationi

    Entry nameiGSHRC_BRARP
    AccessioniPrimary (citable) accession number: O04955
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 27, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is a redox-active disulfide bond.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3