Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative pectinesterase 52

Gene

PME52

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Pectinesterase (AXX17_At1g11890), Pectinesterase (AXX17_At4g02940), Pectinesterase (AXX17_At5g52320), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Probable pectinesterase 15 (PME15), Putative pectinesterase 14 (PME14), Pectinesterase (At3g10720), Pectinesterase (AXX17_At2g33410), Probable pectinesterase 55 (PME55), Pectinesterase (PMEPCRF), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Pectinesterase (AXX17_At4g18720), Pectinesterase 4 (PME4), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Pectinesterase (AXX17_At1g48290), Pectinesterase (AXX17_At5g47900), Pectinesterase (At3g14310), Pectinesterase (AXX17_At4g38050), Pectinesterase/pectinesterase inhibitor 18 (PME18), Pectinesterase (AXX17_At3g43380), Uncharacterized protein (AXX17_At5g64270), Pectinesterase (At5g26810), Putative pectinesterase 10 (PME10), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Pectinesterase (F14I3.7), Plant invertase/pectin methylesterase inhibitor superfamily (At3g49220), Pectinesterase (At3g49220), Probable pectinesterase 29 (PME29), Pectinesterase (AXX17_At4g38040), Pectinesterase (At5g20860), Probable pectinesterase 8 (PME8), Uncharacterized protein (AXX17_At5g27870), Pectinesterase (AXX17_At2g45340), Pectinesterase 31 (PME31), Pectinesterase (AXX17_At3g41380), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At5g04430), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Pectinesterase QRT1 (QRT1), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase (AXX17_At3g53530), Pectinesterase PPME1 (PPME1), Pectinesterase (AXX17_At3g56460), Pectinesterase (AXX17_At3g06710), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 52 (PME52), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At2g40510), Pectinesterase (At1g53840), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase 30 (PME30), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Pectinesterase (PMEPCRA), Pectinesterase (AXX17_At3g26070), Probable pectinesterase 68 (PME68), Pectinesterase, Pectinesterase (AXX17_At3g14900), Probable pectinesterase 67 (PME67), Probable pectinesterase 56 (PME56), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Pectinesterase, Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase (AXX17_At2g22270), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Probable pectinesterase 66 (PME66), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At5g45990), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Probable pectinesterase 50 (PME50), Pectinesterase (AXX17_At3g17940), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Pectinesterase (AXX17_At1g11920), Pectinesterase (AXX17_At3g30550), PME26 (AXX17_At3g14890), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase (AXX17_At2g44790), Pectinesterase (AXX17_At5g19650), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Pectinesterase (AXX17_At3g31890), Pectinesterase (AXX17_At4g02900), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Pectinesterase 1 (PME1), Putative pectinesterase 63 (PME63), Uncharacterized protein (AXX17_At3g05180), Pectinesterase 2 (PME2), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Pectinesterase (AXX17_At2g22280), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Pectinesterase (AXX17_At4g02930), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Pectinesterase/pectinesterase inhibitor 3 (PME3), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At5g50300), Pectinesterase (AXX17_At1g11900), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Putative pectinesterase 57 (PME57), Pectinesterase (PME44), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At3g55110), Pectinesterase (AXX17_At3g10570), Putative pectinesterase 11 (PME11), Plant invertase/pectin methylesterase inhibitor superfamily (At5g09760), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At4g06570), Pectinesterase (AXX17_At5g50310), Pectinesterase (AXX17_At2g33420), Pectinesterase, Pectinesterase (At4g15980), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At3g05190), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g00230), Uncharacterized protein (AXX17_At5g09310), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At1g04730), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Pectinesterase (AXX17_At5g26780)
  2. Pectate lyase (AXX17_At3g26720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 18 (At4g24780), Pectate lyase (At3g27400), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Pectate lyase (AXX17_At3g09180), Putative pectate lyase 14 (At4g13210), Pectate lyase (At3g55140), Pectate lyase (AXX17_At5g54880), Pectate lyase (AXX17_At4g15660), Pectate lyase (At5g04310), Probable pectate lyase 22 (At5g63180), Pectate lyase (At5g09280), Pectate lyase (AXX17_At3g00310), Pectate lyase (At3g07010), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 4 (At1g30350), Pectate lyase (AXX17_At2g01680), Putative pectate lyase 21 (At5g55720), Pectate lyase (At4g13210), Pectate lyase (At5g04310), Pectate lyase (AXX17_At5g03700), Pectate lyase (At4g13710), Pectate lyase (AXX17_At3g49720), Pectate lyase, Pectate lyase, Pectate lyase (AXX17_At1g12300), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 1 (At1g04680), Pectate lyase (At3g07010), Pectate lyase (AXX17_At5g08800), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At5g62760), Pectate lyase (AXX17_At3g47590), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At4g25600), Pectate lyase (AXX17_At1g61710), Probable pectate lyase 20 (At5g48900), Pectate lyase (AXX17_At5g47570), Probable pectate lyase 6 (At2g02720), Pectate lyase (AXX17_At3g06910), Pectate lyase (At1g14420), Pectate lyase (At3g01270), Pectate lyase, Pectate lyase (At3g09540), Pectate lyase (AXX17_At1g04020), Pectate lyase (At3g01270), Pectate lyase (At3g55140), Putative pectate lyase 2 (At1g11920), Pectate lyase, Pectate lyase (AXX17_At1g30780), Probable pectate lyase 19 (At5g15110), Pectate lyase (AXX17_At4g28670), Pectate lyase (F11F8_12), Probable pectate lyase 13 (PMR6), Probable pectate lyase 3 (AT59), Pectate lyase (AXX17_At3g26200), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 12 (At3g53190), Pectate lyase, Pectate lyase (AXX17_At4g25610), Probable pectate lyase 15 (At4g13710), Pectate lyase (At3g53190), Putative pectate lyase 11 (At3g27400), Pectate lyase (AXX17_At1g15010), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g29900), Pectate lyase (AXX17_At5g14600), Probable pectate lyase 10 (At3g24670)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81SubstrateBy similarity1
Sitei122Transition state stabilizerBy similarity1
Active sitei123Proton donorPROSITE-ProRule annotation1
Active sitei144NucleophilePROSITE-ProRule annotation1
Binding sitei206SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl esterase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G26810-MONOMER
UniPathwayiUPA00545; UER00823

Names & Taxonomyi

Protein namesi
Recommended name:
Putative pectinesterase 52 (EC:3.1.1.11)
Short name:
PE 52
Alternative name(s):
Pectin methylesterase 52
Short name:
AtPME52
Gene namesi
Name:PME52
Synonyms:ARATH52
Ordered Locus Names:At5g26810
ORF Names:F2P16.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G26810
TAIRilocus:2148508 AT5G26810

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000037170022 – 293Putative pectinesterase 52Add BLAST272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi160N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO04953

Expressioni

Tissue specificityi

Expressed in siliques.1 Publication

Developmental stagei

Expressed during late developmental phases of siliques.1 Publication

Gene expression databases

ExpressionAtlasiO04953 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G26810.1

Structurei

3D structure databases

ProteinModelPortaliO04953
SMRiO04953
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JA04 Eukaryota
COG4677 LUCA
HOGENOMiHOG000217409
InParanoidiO04953
OMAiWISNIPR
OrthoDBiEOG09360GRE
PhylomeDBiO04953

Family and domain databases

Gene3Di2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
SUPFAMiSSF51126 SSF51126, 1 hit
PROSITEiView protein in PROSITE
PS00503 PECTINESTERASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04953-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPCLFIFIAL LLSSCIGTLK ALDQTCGNKV VNTIVVDQAG SGEGQRVTTI
60 70 80 90 100
TYNGHAATDV SSTFTSYPSH IVVRNLSIMN TYNRLTSLTK ANGMSWDIKP
110 120 130 140 150
AVAISVYGDK SAFYNCDFLG LQDTVWDNLG RHHFKNCYIE GAIDFIFGSG
160 170 180 190 200
QSVYEDCHIN ATAGALASKV SFGYITAQGR SSDSDPSGFV FLRGSVSGST
210 220 230 240 250
SVYLGRAYGP FSRVIFIQTD LSSVVHPEGW YSWHYGGYEM SFTYAEVECK
260 270 280 290
GAGSDMSRRV PWIDKLHSFY TKQQFSISNF IDQDQWISNI PRF
Length:293
Mass (Da):32,350
Last modified:May 5, 2009 - v2
Checksum:i917A69C10E7453F8
GO

Sequence cautioni

The sequence AAB61046 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007270 Genomic DNA Translation: AAB61046.1 Sequence problems.
CP002688 Genomic DNA No translation available.
PIRiT01761

Genome annotation databases

KEGGiath:AT5G26810

Similar proteinsi

Entry informationi

Entry nameiPME52_ARATH
AccessioniPrimary (citable) accession number: O04953
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: April 25, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health