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O04951

- PP2A5_ARATH

UniProt

O04951 - PP2A5_ARATH

Protein

Serine/threonine-protein phosphatase PP2A-5 catalytic subunit

Gene

PP2A5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi55 – 551Manganese 1By similarity
    Metal bindingi57 – 571Manganese 1By similarity
    Metal bindingi83 – 831Manganese 1By similarity
    Metal bindingi83 – 831Manganese 2By similarity
    Metal bindingi115 – 1151Manganese 2By similarity
    Active sitei116 – 1161Proton donorBy similarity
    Metal bindingi165 – 1651Manganese 2By similarity
    Metal bindingi239 – 2391Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: TAIR

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. regulation of auxin polar transport Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G69960-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP2A-5 catalytic subunit (EC:3.1.3.16)
    Alternative name(s):
    Protein phosphatase 2A isoform 5
    Gene namesi
    Name:PP2A5
    Ordered Locus Names:At1g69960
    ORF Names:F20P5.30, T17F3.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G69960.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 307307Serine/threonine-protein phosphatase PP2A-5 catalytic subunitPRO_0000058856Add
    BLAST

    Post-translational modificationi

    Ubiquitinated. CHIP-mediated ubiquitination enhances phosphatase activity after an abiotic stress such as low temperature or darkness By similarity.By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PRIDEiO04951.

    Expressioni

    Gene expression databases

    GenevestigatoriO04951.

    Interactioni

    Subunit structurei

    PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families). Also interacts with CHIP and TAF12B.3 Publications

    Protein-protein interaction databases

    BioGridi28554. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO04951.
    SMRiO04951. Positions 5-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

    Phylogenomic databases

    HOGENOMiHOG000172696.
    InParanoidiO04951.
    KOiK04382.
    OMAiVEPETTR.
    PhylomeDBiO04951.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O04951-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPATGDIDR QIEQLMECKA LSETEVKMLC EHAKTILVEE YNVQPVKCPV    50
    TVCGDIHGQF YDLIELFRIG GSSPDTNYLF MGDYVDRGYY SVETVSLLVA 100
    LKVRYRDRLT ILRGNHESRQ ITQVYGFYDE CLRKYGNANV WKHFTDLFDY 150
    LPLTALIESQ VFCLHGGLSP SLDTLDNIRS LDRIQEVPHE GPMCDLLWSD 200
    PDDRCGWGIS PRGAGYTFGQ DIATQFNHTN GLSLISRAHQ LVMEGFNWCQ 250
    EKNVVTVFSA PNYCYRCGNM AAILEIGENM DQNFLQFDPA PRQVEPETTR 300
    KTPDYFL 307
    Length:307
    Mass (Da):35,042
    Last modified:July 1, 1997 - v1
    Checksum:iD00149EA0E20C82F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U39568 mRNA. Translation: AAC49668.1.
    AC002062 Genomic DNA. Translation: AAB61116.1.
    AC010675 Genomic DNA. Translation: AAG52565.1.
    CP002684 Genomic DNA. Translation: AEE35002.1.
    BT025871 mRNA. Translation: ABF85773.1.
    AK227054 mRNA. Translation: BAE99114.1.
    PIRiB96722.
    RefSeqiNP_177154.1. NM_105665.3.
    UniGeneiAt.20106.

    Genome annotation databases

    EnsemblPlantsiAT1G69960.1; AT1G69960.1; AT1G69960.
    GeneIDi843333.
    KEGGiath:AT1G69960.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U39568 mRNA. Translation: AAC49668.1 .
    AC002062 Genomic DNA. Translation: AAB61116.1 .
    AC010675 Genomic DNA. Translation: AAG52565.1 .
    CP002684 Genomic DNA. Translation: AEE35002.1 .
    BT025871 mRNA. Translation: ABF85773.1 .
    AK227054 mRNA. Translation: BAE99114.1 .
    PIRi B96722.
    RefSeqi NP_177154.1. NM_105665.3.
    UniGenei At.20106.

    3D structure databases

    ProteinModelPortali O04951.
    SMRi O04951. Positions 5-291.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 28554. 3 interactions.

    Proteomic databases

    PRIDEi O04951.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G69960.1 ; AT1G69960.1 ; AT1G69960 .
    GeneIDi 843333.
    KEGGi ath:AT1G69960.

    Organism-specific databases

    TAIRi AT1G69960.

    Phylogenomic databases

    HOGENOMi HOG000172696.
    InParanoidi O04951.
    KOi K04382.
    OMAi VEPETTR.
    PhylomeDBi O04951.

    Enzyme and pathway databases

    BioCyci ARA:AT1G69960-MONOMER.

    Gene expression databases

    Genevestigatori O04951.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a novel isoform of a type 2A serine/threonine protein phosphatase from Arabidopsis thaliana."
      Stamey R.T., Rundle S.J.
      Plant Gene Register PGR95-116
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Molecular characterization of the B' regulatory subunit gene family of Arabidopsis protein phosphatase 2A."
      Haynes J.G., Hartung A.J., Hendershot J.D. III, Passingham R.S., Rundle S.J.
      Eur. J. Biochem. 260:127-136(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AALPHA.
    7. "AtCHIP functions as an E3 ubiquitin ligase of protein phosphatase 2A subunits and alters plant response to abscisic acid treatment."
      Luo J., Shen G., Yan J., He C., Zhang H.
      Plant J. 46:649-657(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHIP.
    8. "Arabidopsis PPP family of serine/threonine phosphatases."
      Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
      Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    9. "Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response, including ERF1 induction."
      Robles L.M., Wampole J.S., Christians M.J., Larsen P.B.
      J. Exp. Bot. 58:2627-2639(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TAF12B.
      Strain: cv. Wassilewskija.

    Entry informationi

    Entry nameiPP2A5_ARATH
    AccessioniPrimary (citable) accession number: O04951
    Secondary accession number(s): Q1EC63, Q42544
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3