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Protein

Serine/threonine-protein phosphatase PP2A-5 catalytic subunit

Gene

PP2A5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Manganese 1By similarity
Metal bindingi57 – 571Manganese 1By similarity
Metal bindingi83 – 831Manganese 1By similarity
Metal bindingi83 – 831Manganese 2By similarity
Metal bindingi115 – 1151Manganese 2By similarity
Active sitei116 – 1161Proton donorBy similarity
Metal bindingi165 – 1651Manganese 2By similarity
Metal bindingi239 – 2391Manganese 2By similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  • dephosphorylation Source: GOC
  • regulation of auxin polar transport Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G69960-MONOMER.
ReactomeiREACT_273308. ERK/MAPK targets.
REACT_286793. PP2A-mediated dephosphorylation of key metabolic factors.
REACT_293115. Glycolysis.
REACT_310958. ERKs are inactivated.
REACT_318739. Integration of energy metabolism.
REACT_319752. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP2A-5 catalytic subunit (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2A isoform 5
Gene namesi
Name:PP2A5
Ordered Locus Names:At1g69960
ORF Names:F20P5.30, T17F3.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G69960.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307Serine/threonine-protein phosphatase PP2A-5 catalytic subunitPRO_0000058856Add
BLAST

Post-translational modificationi

Ubiquitinated. CHIP-mediated ubiquitination enhances phosphatase activity after an abiotic stress such as low temperature or darkness (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiO04951.

Interactioni

Subunit structurei

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families). Also interacts with CHIP and TAF12B.3 Publications

Protein-protein interaction databases

BioGridi28554. 3 interactions.
STRINGi3702.AT1G69960.1.

Structurei

3D structure databases

ProteinModelPortaliO04951.
SMRiO04951. Positions 5-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000172696.
KOiK04382.
OMAiCANQAAM.
PhylomeDBiO04951.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O04951-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPATGDIDR QIEQLMECKA LSETEVKMLC EHAKTILVEE YNVQPVKCPV
60 70 80 90 100
TVCGDIHGQF YDLIELFRIG GSSPDTNYLF MGDYVDRGYY SVETVSLLVA
110 120 130 140 150
LKVRYRDRLT ILRGNHESRQ ITQVYGFYDE CLRKYGNANV WKHFTDLFDY
160 170 180 190 200
LPLTALIESQ VFCLHGGLSP SLDTLDNIRS LDRIQEVPHE GPMCDLLWSD
210 220 230 240 250
PDDRCGWGIS PRGAGYTFGQ DIATQFNHTN GLSLISRAHQ LVMEGFNWCQ
260 270 280 290 300
EKNVVTVFSA PNYCYRCGNM AAILEIGENM DQNFLQFDPA PRQVEPETTR

KTPDYFL
Length:307
Mass (Da):35,042
Last modified:July 1, 1997 - v1
Checksum:iD00149EA0E20C82F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39568 mRNA. Translation: AAC49668.1.
AC002062 Genomic DNA. Translation: AAB61116.1.
AC010675 Genomic DNA. Translation: AAG52565.1.
CP002684 Genomic DNA. Translation: AEE35002.1.
BT025871 mRNA. Translation: ABF85773.1.
AK227054 mRNA. Translation: BAE99114.1.
PIRiB96722.
RefSeqiNP_177154.1. NM_105665.3.
UniGeneiAt.20106.

Genome annotation databases

EnsemblPlantsiAT1G69960.1; AT1G69960.1; AT1G69960.
GeneIDi843333.
KEGGiath:AT1G69960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39568 mRNA. Translation: AAC49668.1.
AC002062 Genomic DNA. Translation: AAB61116.1.
AC010675 Genomic DNA. Translation: AAG52565.1.
CP002684 Genomic DNA. Translation: AEE35002.1.
BT025871 mRNA. Translation: ABF85773.1.
AK227054 mRNA. Translation: BAE99114.1.
PIRiB96722.
RefSeqiNP_177154.1. NM_105665.3.
UniGeneiAt.20106.

3D structure databases

ProteinModelPortaliO04951.
SMRiO04951. Positions 5-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28554. 3 interactions.
STRINGi3702.AT1G69960.1.

Proteomic databases

PRIDEiO04951.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G69960.1; AT1G69960.1; AT1G69960.
GeneIDi843333.
KEGGiath:AT1G69960.

Organism-specific databases

TAIRiAT1G69960.

Phylogenomic databases

HOGENOMiHOG000172696.
KOiK04382.
OMAiCANQAAM.
PhylomeDBiO04951.

Enzyme and pathway databases

BioCyciARA:AT1G69960-MONOMER.
ReactomeiREACT_273308. ERK/MAPK targets.
REACT_286793. PP2A-mediated dephosphorylation of key metabolic factors.
REACT_293115. Glycolysis.
REACT_310958. ERKs are inactivated.
REACT_318739. Integration of energy metabolism.
REACT_319752. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiO04951.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a novel isoform of a type 2A serine/threonine protein phosphatase from Arabidopsis thaliana."
    Stamey R.T., Rundle S.J.
    Plant Gene Register PGR95-116
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Molecular characterization of the B' regulatory subunit gene family of Arabidopsis protein phosphatase 2A."
    Haynes J.G., Hartung A.J., Hendershot J.D. III, Passingham R.S., Rundle S.J.
    Eur. J. Biochem. 260:127-136(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AALPHA.
  7. "AtCHIP functions as an E3 ubiquitin ligase of protein phosphatase 2A subunits and alters plant response to abscisic acid treatment."
    Luo J., Shen G., Yan J., He C., Zhang H.
    Plant J. 46:649-657(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHIP.
  8. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  9. "Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response, including ERF1 induction."
    Robles L.M., Wampole J.S., Christians M.J., Larsen P.B.
    J. Exp. Bot. 58:2627-2639(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TAF12B.
    Strain: cv. Wassilewskija.

Entry informationi

Entry nameiPP2A5_ARATH
AccessioniPrimary (citable) accession number: O04951
Secondary accession number(s): Q1EC63, Q42544
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: July 22, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.