O04951 (PP2A5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP2A-5 catalytic subunit
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 2A isoform 5

Gene names

Name:	PP2A5
Ordered Locus Names:	At1g69960
ORF Names:	F20P5.30, T17F3.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subunit structure	PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families). Also interacts with CHIP. Ref.6 Ref.7
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Ubiquitinated. CHIP-mediated ubiquitination enhances phosphatase activity after an abiotic stress such as low temperature or darkness By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-2A subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of auxin polar transport Inferred from mutant phenotype PubMed 8641277. Source: TAIR
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from sequence or structural similarity PubMed 8382968. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Serine/threonine-protein phosphatase PP2A-5 catalytic subunit		PRO_0000058856
Sites
	Active site	116	1	Proton donor By similarity
	Metal binding	55	1	Iron By similarity
	Metal binding	57	1	Iron By similarity
	Metal binding	83	1	Iron By similarity
	Metal binding	83	1	Manganese By similarity
	Metal binding	115	1	Manganese By similarity
	Metal binding	165	1	Manganese By similarity
	Metal binding	239	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	O04951 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: D00149EA0E20C82F	FASTA	307	35,042
	10 20 30 40 50 60 MPPATGDIDR QIEQLMECKA LSETEVKMLC EHAKTILVEE YNVQPVKCPV TVCGDIHGQF 70 80 90 100 110 120 YDLIELFRIG GSSPDTNYLF MGDYVDRGYY SVETVSLLVA LKVRYRDRLT ILRGNHESRQ 130 140 150 160 170 180 ITQVYGFYDE CLRKYGNANV WKHFTDLFDY LPLTALIESQ VFCLHGGLSP SLDTLDNIRS 190 200 210 220 230 240 LDRIQEVPHE GPMCDLLWSD PDDRCGWGIS PRGAGYTFGQ DIATQFNHTN GLSLISRAHQ 250 260 270 280 290 300 LVMEGFNWCQ EKNVVTVFSA PNYCYRCGNM AAILEIGENM DQNFLQFDPA PRQVEPETTR KTPDYFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a novel isoform of a type 2A serine/threonine protein phosphatase from Arabidopsis thaliana." Stamey R.T., Rundle S.J. Plant Gene Register PGR95-116 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Molecular characterization of the B' regulatory subunit gene family of Arabidopsis protein phosphatase 2A." Haynes J.G., Hartung A.J., Hendershot J.D. III, Passingham R.S., Rundle S.J. Eur. J. Biochem. 260:127-136(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH AALPHA.
[7]	"AtCHIP functions as an E3 ubiquitin ligase of protein phosphatase 2A subunits and alters plant response to abscisic acid treatment." Luo J., Shen G., Yan J., He C., Zhang H. Plant J. 46:649-657(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CHIP.
[8]	"Arabidopsis PPP family of serine/threonine phosphatases." Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C. Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U39568 mRNA. Translation: AAC49668.1. AC002062 Genomic DNA. Translation: AAB61116.1. AC010675 Genomic DNA. Translation: AAG52565.1. CP002684 Genomic DNA. Translation: AEE35002.1. BT025871 mRNA. Translation: ABF85773.1. AK227054 mRNA. Translation: BAE99114.1.
IPI	IPI00542605.
PIR	B96722.
RefSeq	NP_177154.1. NM_105665.3.
UniGene	At.20106.
3D structure databases
ProteinModelPortal	O04951.
SMR	O04951. Positions 5-291.
ModBase	Search...
Proteomic databases
PRIDE	O04951.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G69960.1; AT1G69960.1; AT1G69960.
GeneID	843333.
KEGG	ath:AT1G69960.
Organism-specific databases
TAIR	At1g69960.
Phylogenomic databases
HOGENOM	HOG000172696.
InParanoid	O04951.
KO	K04382.
OMA	VEPETTR.
ProtClustDB	CLSN2679580.
Gene expression databases
Genevestigator	O04951.
GermOnline	AT1G69960. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP2A5_ARATH

Accession

Primary (citable) accession number: O04951
Secondary accession number(s): Q1EC63, Q42544

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program