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O04951 (PP2A5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase PP2A-5 catalytic subunit

EC=3.1.3.16
Alternative name(s):
Protein phosphatase 2A isoform 5
Gene names
Name:PP2A5
Ordered Locus Names:At1g69960
ORF Names:F20P5.30, T17F3.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Subunit structure

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families). Also interacts with CHIP and TAF12B. Ref.6 Ref.7 Ref.9

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Ubiquitinated. CHIP-mediated ubiquitination enhances phosphatase activity after an abiotic stress such as low temperature or darkness By similarity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-2A subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 307307Serine/threonine-protein phosphatase PP2A-5 catalytic subunit
PRO_0000058856

Sites

Active site1161Proton donor By similarity
Metal binding551Manganese 1 By similarity
Metal binding571Manganese 1 By similarity
Metal binding831Manganese 1 By similarity
Metal binding831Manganese 2 By similarity
Metal binding1151Manganese 2 By similarity
Metal binding1651Manganese 2 By similarity
Metal binding2391Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
O04951 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: D00149EA0E20C82F

FASTA30735,042
        10         20         30         40         50         60 
MPPATGDIDR QIEQLMECKA LSETEVKMLC EHAKTILVEE YNVQPVKCPV TVCGDIHGQF 

        70         80         90        100        110        120 
YDLIELFRIG GSSPDTNYLF MGDYVDRGYY SVETVSLLVA LKVRYRDRLT ILRGNHESRQ 

       130        140        150        160        170        180 
ITQVYGFYDE CLRKYGNANV WKHFTDLFDY LPLTALIESQ VFCLHGGLSP SLDTLDNIRS 

       190        200        210        220        230        240 
LDRIQEVPHE GPMCDLLWSD PDDRCGWGIS PRGAGYTFGQ DIATQFNHTN GLSLISRAHQ 

       250        260        270        280        290        300 
LVMEGFNWCQ EKNVVTVFSA PNYCYRCGNM AAILEIGENM DQNFLQFDPA PRQVEPETTR 


KTPDYFL 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of a novel isoform of a type 2A serine/threonine protein phosphatase from Arabidopsis thaliana."
Stamey R.T., Rundle S.J.
Plant Gene Register PGR95-116
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Molecular characterization of the B' regulatory subunit gene family of Arabidopsis protein phosphatase 2A."
Haynes J.G., Hartung A.J., Hendershot J.D. III, Passingham R.S., Rundle S.J.
Eur. J. Biochem. 260:127-136(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH AALPHA.
[7]"AtCHIP functions as an E3 ubiquitin ligase of protein phosphatase 2A subunits and alters plant response to abscisic acid treatment."
Luo J., Shen G., Yan J., He C., Zhang H.
Plant J. 46:649-657(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CHIP.
[8]"Arabidopsis PPP family of serine/threonine phosphatases."
Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[9]"Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response, including ERF1 induction."
Robles L.M., Wampole J.S., Christians M.J., Larsen P.B.
J. Exp. Bot. 58:2627-2639(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TAF12B.
Strain: cv. Wassilewskija.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U39568 mRNA. Translation: AAC49668.1.
AC002062 Genomic DNA. Translation: AAB61116.1.
AC010675 Genomic DNA. Translation: AAG52565.1.
CP002684 Genomic DNA. Translation: AEE35002.1.
BT025871 mRNA. Translation: ABF85773.1.
AK227054 mRNA. Translation: BAE99114.1.
PIRB96722.
RefSeqNP_177154.1. NM_105665.3.
UniGeneAt.20106.

3D structure databases

ProteinModelPortalO04951.
SMRO04951. Positions 5-291.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid28554. 3 interactions.

Proteomic databases

PRIDEO04951.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G69960.1; AT1G69960.1; AT1G69960.
GeneID843333.
KEGGath:AT1G69960.

Organism-specific databases

TAIRAT1G69960.

Phylogenomic databases

HOGENOMHOG000172696.
InParanoidO04951.
KOK04382.
OMAVEPETTR.
PhylomeDBO04951.

Enzyme and pathway databases

BioCycARA:AT1G69960-MONOMER.

Gene expression databases

GenevestigatorO04951.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP2A5_ARATH
AccessionPrimary (citable) accession number: O04951
Secondary accession number(s): Q1EC63, Q42544
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names