Reviewed, UniProtKB/Swiss-Prot O04937 (DHEA_NICPL)

Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase A
      Short name=GDH A
    EC=1.4.1.3

Gene names

Name:

GDHA

Organism

Nicotiana plumbaginifolia (Leadwort-leaved tobacco)

Taxonomic identifier

4092 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Glutamate dehydrogenase A		PRO_0000182750
Sites
	Active site	102	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O04937-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 5CCF132AF2103904	FASTA	411	44,796
	10 20 30 40 50 60 MNALAATNRN FRQAARILGL DSKLEKSLLI PFREIKVECT IPKDDGTLVS YVGFRVQHDN 70 80 90 100 110 120 ARGPMKGGIR YHPEVDLDEV NALAQLMTWK TAVADIPYGG AKGGIGCKPK DLSKSELERL 130 140 150 160 170 180 TRVFTQKIHD LIGINTDVPA PDMGTNAQTM AWILDEYSKF HGHSPAIVTG KPIDLGGSLG 190 200 210 220 230 240 REAATGRGVV YATEALLAEY GKNIKDLTFA IQGFGNVGAW AAKLIHERGG KVIAVSDITG 250 260 270 280 290 300 AVKNPNGLDI PALLNHKEAT GKLIDFSGGD VMNSDEVLTH ECDVLIPCAL GGVLNRENAD 310 320 330 340 350 360 NVKAKFIIEA ANHPTDPEAD EILCKKGIVI LPDIYANAGG VTVSYFEWVQ NIQGFMWDEE 370 380 390 400 410 KVNRELRKYM TKAFHNLKNM CQSHNCSLRM GAFTLGVNRV ARATTLRGWE A

References

[1]	"Isolation and characterization of two cDNA clones encoding for glutamate dehydrogenase in Nicotiana plumbaginifolia." Ficarelli A., Tassi F., Restivo F.M. Plant Cell Physiol. 40:339-342(1999) [PubMed: 10353219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[2]	"Genomic organisation of GDH genes in Nicotiana plumbaginifolia." Restivo F.M., Panizzi N., De Monte G., Ficarelli A. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y08292 mRNA. Translation: CAA69600.1. AJ277949 Genomic DNA. Translation: CAB94836.1.
PIR	T16981.
3D structure databases
HSSP	HSSP built from PDB template 1B26 based on UniProtKB P96110.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.3. 257040.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHEA_NICPL

Accession

Primary (citable) accession number: O04937

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 1, 1997
Last modified:	September 22, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)