O04931 (AGLU_BETVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alpha-glucosidase EC=3.2.1.20 Alternative name(s): Maltase
Organism	Beta vulgaris (Sugar beet)
Taxonomic identifier	161934 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Beta

Protein attributes

Sequence length	913 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High activity for alpha-glucan.
Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the glycosyl hydrolase 31 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro maltose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 913	885	Alpha-glucosidase		PRO_0000018582
Sites
	Active site	469	1	Ref.2
	Active site	472	1	By similarity
	Active site	568	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	54	1	N-linked (GlcNAc...) Potential
	Glycosylation	404	1	N-linked (GlcNAc...) Potential
	Glycosylation	495	1	N-linked (GlcNAc...) Potential
	Glycosylation	517	1	N-linked (GlcNAc...) Potential
	Glycosylation	728	1	N-linked (GlcNAc...) Potential
	Glycosylation	823	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O04931 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 02AC4F0B505369CC	FASTA	913	102,117
	10 20 30 40 50 60 MERSKLPRYI CPTLAVVLPL VLCMVVEGAT TSKNDNQGEA IGYGYQVKNA KVDNSTGKSL 70 80 90 100 110 120 TALLQLIRNS PVYGPDIHFL SFTASFEEDD TLRIRFTDAN NRRWEIPNEV LPRPPPPPSP 130 140 150 160 170 180 PPLSSLQHLP KPIPQNQPTT TVLSHPHSDL AFTLFHTTPF GFTIYRKSTH DVLFDATPIP 190 200 210 220 230 240 SNPTTFLIYK DQYLQLSSSL PAQQAHLYGL GEHTKPTFQL AHNQILTLWN ADIASFNRDL 250 260 270 280 290 300 NLYGSHPFYM DVRSSPMVGS THGVFLLNSN GMDVEYTGDR ITYKVIGGII DLYIFAGRTP 310 320 330 340 350 360 EMVLDQYTKL IGRPAPMPYW AFGFHQCRWG YRDVNEIETV VDKYAEARIP LEVMWTDIDY 370 380 390 400 410 420 MDAFKDFTLD PVHFPLDKMQ QFVTKLHRNG QRYVPILDPG INTNKSYGTF IRGMQSNVFI 430 440 450 460 470 480 KRNGNPYLGS VWPGPVYYPD FLDPAARSFW VDEIKRFRDI LPIDGIWIDM NEASNFITSA 490 500 510 520 530 540 PTPGSTLDNP PYKINNSGGR VPINSKTIPA TAMHYGNVTE YNAHNLYGFL ESQATREALV 550 560 570 580 590 600 RPATRGPFLL SRSTFAGSGK YTAHWTGDNA ARWDDLQYSI PTMLNFGLFG MPMIGADICG 610 620 630 640 650 660 FAESTTEELC CRWIQLGAFY PFSRDHSARD TTHQELYLWE SVAASARTVL GLRYELLPYY 670 680 690 700 710 720 YTLMYDANLR GSPIARPLSF TFPDDVATYG ISSQFLIGRG IMVSPVLQPG SSIVNAYSPR 730 740 750 760 770 780 GNWVSLSNYT SSVSVSAGTY VSLSAPPDHI NVHIHEGNIV AMQGEAMTTQ AARSTPFHLL 790 800 810 820 830 840 VVMSDHVAST GELFLDNGIE MDIGGPGGKW TLVRFFAESG INNLTISSEV VNRGYAMSQR 850 860 870 880 890 900 WVMDKITILG LKRRVKIKEY TVQKDAGAIK VKGLGRRTSS HNQGGFFVSV ISDLRQLVGQ 910 AFKLELEFEG ATR

References

[1]	"Cloning and sequencing of a cDNA encoding alpha-glucosidase from sugar beet." Matsui H., Iwanami S., Ito H., Mori H., Honma M., Chiba S. Biosci. Biotechnol. Biochem. 61:875-880(1997) [PubMed: 9178565] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. NK-152.
[2]	"Chemical modification and amino acid sequence of active site in sugar beet alpha-glucosidase." Iwanami S., Matsui H., Kimura A., Ito H., Mori H., Honma M., Chiba S. Biosci. Biotechnol. Biochem. 59:459-463(1995) [PubMed: 7766184] [Abstract] Cited for: ACTIVE SITE, PROTEIN SEQUENCE OF 464-472.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D89615 mRNA. Translation: BAA20343.1.
PIR	JC5463.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH31. Glycoside Hydrolase Family 31.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011013. Glyco_hydro-type_carb-bd. IPR000322. Glyco_hydro_31. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR22762. Glyco_hydro_31. 1 hit.
Pfam	PF01055. Glyco_hydro_31. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit. PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGLU_BETVU

Accession

Primary (citable) accession number: O04931

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	May 31, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program