Reviewed, UniProtKB/Swiss-Prot O04921 (HEMH2_ARATH)

Names and origin

Protein names

Recommended name:
    Ferrochelatase-2, chloroplastic
    EC=4.99.1.1
Alternative name(s):
    Ferrochelatase II
    Protoheme ferro-lyase 2
    Heme synthetase 2

Gene names

Ordered Locus Names:	At2g30390
ORF Names:	T06B20.24, T9D9.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. May have a role in dealing with oxidative stress.
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+.
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.
Subcellular location	Plastid › chloroplast membrane; Peripheral membrane protein. Plastid › chloroplast thylakoid membrane; Peripheral membrane protein Ref.4.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide
Ligand	Iron
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis, light harvesting Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Ref.1 Inferred from direct assay. Source: TAIR extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferrochelatase activity Ref.1 Inferred from genetic interaction. Source: TAIR iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 512		Ferrochelatase-2, chloroplastic		PRO_0000008881
Regions
	Compositional bias	9 – 19	11	Poly-Ser
	Compositional bias	23 – 26	4	Poly-Pro
	Compositional bias	80 – 83	4	Poly-Ser
	Compositional bias	90 – 93	4	Poly-Ser
Experimental info
	Sequence conflict	156 – 159	4	SKEG → ARR in CAA73614. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O04921-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 521B1FC596E844A7	FASTA	512	56,619
	10 20 30 40 50 60 MNCPAMTASP SSSSSSSYST FRPPPPLLPQ LSNDSQRSVV MHCTRLPFEA FAATSSNRLL 70 80 90 100 110 120 GKHSLPLRAA LVTSNPLNIS SSSVISDAIS SSSVITDDAK IGVLLLNLGG PETLDDVQPF 130 140 150 160 170 180 LFNLFADPDI IRLPPVFQFL QKPLAQFISV ARAPKSKEGY ASIGGGSPLR HITDAQAEEL 190 200 210 220 230 240 RKCLWEKNVP AKVYVGMRYW HPFTEEAIEQ IKRDGITKLV VLPLYPQFSI STSGSSLRLL 250 260 270 280 290 300 ERIFREDEYL VNMQHTVIPS WYQREGYIKA MANLIQSELG KFGSPNQVVI FFSAHGVPLA 310 320 330 340 350 360 YVEEAGDPYK AEMEECVDLI MEELDKRKIT NAYTLAYQSR VGPVEWLKPY TEEAITELGK 370 380 390 400 410 420 KGVENLLAVP ISFVSEHIET LEEIDVEYKE LALKSGIKNW GRVPALGTEP MFISDLADAV 430 440 450 460 470 480 VESLPYVGAM AVSNLEARQS LVPLGSVEEL LATYDSQRRE LPAPVTMWEW GWTKSAETWN 490 500 510 GRAAMLAVLA LLVLEVTTGK GFLHQWGILP SL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two different genes encode ferrochelatase in Arabidopsis: mapping, expression and subcellular targeting of the precursor proteins." Chow K.-S., Singh D.P., Walker A., Smith A.G. Plant J. 15:531-541(1998) [PubMed: 9753778] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Seedling.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Molecular localisation of ferrochelatase in higher plant chloroplasts." Roper J.M., Smith A.G. Eur. J. Biochem. 246:32-37(1997) [PubMed: 9210462] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y13156 mRNA. Translation: CAA73614.1. U93215 Genomic DNA. Translation: AAB63095.1. AC002338 Genomic DNA. Translation: AAM14820.1. BT000465 mRNA. Translation: AAN17442.1. BT008877 mRNA. Translation: AAP68316.1.
IPI	IPI00534550.
PIR	H84707.
RefSeq	NP_180598.1.
UniGene	At.22966 Rsa.2561
3D structure databases
SMR	O04921. Positions 99-425.
ModBase	Search...
Protein-protein interaction databases
STRING	O04921.
Proteomic databases
PRIDE	O04921.
Genome annotation databases
GeneID	817589.
GenomeReviews	Gene locus AT2G30390 in contig CT485783_GR.
KEGG	ath:AT2G30390.
NMPDR	fig|3702.1.peg.10090.
Organism-specific databases
TAIR	At2g30390.
Phylogenomic databases
eggNOG	KOG1321.
HOGENOM	HBG697135.
InParanoid	O04921.
OMA	WLQKPLA.
PhylomeDB	O04921.
Enzyme and pathway databases
BRENDA	4.99.1.1. 302.
Gene expression databases
ArrayExpress	O04921.
Genevestigator	O04921.
GermOnline	AT2G30390. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH2_ARATH

Accession

Primary (citable) accession number: O04921
Secondary accession number(s): O23623, Q7GB39

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	February 9, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)