O04916 (ACOC_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Aconitate hydratase, cytoplasmic Short name=Aconitase EC=4.2.1.3 Alternative name(s): Citrate hydro-lyase
Organism	Solanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	616 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the isomerization of citrate to isocitrate via cis-aconitate By similarity.
Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; glyoxylate and dicarboxylate metabolism.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW citrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC isocitrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 616	›616	Aconitate hydratase, cytoplasmic		PRO_0000076654
Regions
	Region	502 – 503	2	Substrate binding By similarity
Sites
	Metal binding	159	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	225	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	228	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	258	1	Substrate By similarity
	Binding site	263	1	Substrate By similarity
	Binding site	421	1	Substrate By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	O04916 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 7563871E3F353521	FASTA	616	67,161
	10 20 30 40 50 60 EFYGGGMSGL SLADRATIAN MAPEYGATMG FFPVDHVTLE YLKLTGRSDE IVGMVEAYLR 70 80 90 100 110 120 ANNMFVDYNE PQQEKVYSSY LNLDLADVEP CLSGPKRPHD RVPLKEMKSD WHALLDNKVG 130 140 150 160 170 180 FKGFAVPKEV QDKVAKFSFH GQPAELKHGS VVIAAITSCT NTSNPSVMLG AALVAKKASE 190 200 210 220 230 240 LGLHVKPWVK TSLAPGSGVV TKYLLKSGLQ KYLNQQGFNI VGYGCTTCIG NSGDLDESVA 250 260 270 280 290 300 SAISENDIVA AAVLSGNRNF EGRVHALTRA NYLASPPLVV AYALAGTVDI DFEKDPIGVG 310 320 330 340 350 360 KDGKDVYFRD IWPSTEEIAE VVQSSVLPDM FKSTYEAITK GNTMWNELSV PTTKLYQWDP 370 380 390 400 410 420 KSTYIHEPPY FKGMTMDPPG PHGVKDAYCL LNFGDSITTD HISPAGSIHK DSPAARYLME 430 440 450 460 470 480 RGVDRRDFNS YGSRRGNDEI MARGTFANIR LVNKLLNGEV GPKTVHVPSG EKLSVFDAAM 490 500 510 520 530 540 KYKSAGQSTI ILAGAEYGSG SSRDWAAKGP MLLGVKAVIA KSFERIHRSN LVGMGIVPLC 550 560 570 580 590 600 FKAGEDADTL GLTGQERYTI DLPENISEIR PGQDVTVQTD TGKSFTCIVR FDTEVELAYF 610 NHGGILQYVI RQLTQR

References

[1]	Surpili M.J. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Desiree.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97012 mRNA. Translation: CAA65735.1.
PIR	T07611.
3D structure databases
ProteinModelPortal	O04916.
SMR	O04916. Positions 1-615.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00227.
Family and domain databases
Gene3D	3.20.19.10. 1 hit. 3.30.499.10. 2 hits. 3.40.1060.10. 1 hit.
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR015937. Acoase/IPM_deHydtase. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
PANTHER	PTHR11670. PTHR11670. 1 hit. PTHR11670:SF1. PTHR11670:SF1. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit. SSF53732. Aconitase_N. 1 hit.
TIGRFAMs	TIGR01341. aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACOC_SOLTU

Accession

Primary (citable) accession number: O04916

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program