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Protein

Dihydroorotase, mitochondrial

Gene

PYR4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 3 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, chloroplastic (CARB), Carbamoyl-phosphate synthase small chain, chloroplastic (CARA)
  2. Aspartate carbamoyltransferase, chloroplastic (PYRB)
  3. Dihydroorotase, mitochondrial (PYR4)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Zinc 1; via tele nitrogenBy similarity1
Metal bindingi46Zinc 1; via tele nitrogenBy similarity1
Metal bindingi130Zinc 1; via carbamate groupBy similarity1
Metal bindingi130Zinc 2; via carbamate groupBy similarity1
Metal bindingi168Zinc 2; via pros nitrogenBy similarity1
Metal bindingi206Zinc 2; via tele nitrogenBy similarity1
Metal bindingi280Zinc 1By similarity1

GO - Molecular functioni

  • dihydroorotase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processPyrimidine biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT4G22930-MONOMER
BRENDAi3.5.2.3 399
UniPathwayiUPA00070; UER00117

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroorotase, mitochondrial (EC:3.5.2.3)
Short name:
DHOase
Gene namesi
Name:PYR4
Ordered Locus Names:At4g22930
ORF Names:F7H19.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G22930
TAIRilocus:2127168 AT4G22930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000029883? – 377Dihydroorotase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei130N6-carboxylysineBy similarity1
Modified residuei223PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO04904
PRIDEiO04904

PTM databases

iPTMnetiO04904

Expressioni

Gene expression databases

ExpressionAtlasiO04904 baseline and differential
GenevisibleiO04904 AT

Interactioni

Protein-protein interaction databases

BioGridi13681, 1 interactor
STRINGi3702.AT4G22930.1

Structurei

3D structure databases

ProteinModelPortaliO04904
SMRiO04904
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2902 Eukaryota
COG0418 LUCA
HOGENOMiHOG000256259
InParanoidiO04904
KOiK01465
OMAiHLRDGAM
OrthoDBiEOG09360EEL
PhylomeDBiO04904

Family and domain databases

CDDicd01294 DHOase, 1 hit
HAMAPiMF_00219 PyrC_classII, 1 hit
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR004721 DHOdimr
IPR002195 Dihydroorotase_CS
IPR032466 Metal_Hydrolase
PANTHERiPTHR43137 PTHR43137, 1 hit
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
PIRSFiPIRSF001237 DHOdimr, 1 hit
SUPFAMiSSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR00856 pyrC_dimer, 1 hit
PROSITEiView protein in PROSITE
PS00482 DIHYDROOROTASE_1, 1 hit
PS00483 DIHYDROOROTASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKTLVSPYS GFGSQKLKFD RSSEKVKTRA VRMELTITQP DDWHLHLRDG
60 70 80 90 100
DLLHAVVPHS ASNFKRAIVM PNLKPPVTST AAAIIYRKFI MKALPSESSF
110 120 130 140 150
DPLMTLYLTD KTLPEEIRLA RESGVVYAVK LYPAGATTNS QDGVTDLFGK
160 170 180 190 200
CLPVLEEMVK QNMPLLVHGE VTDPSIDVFD REKIFIETVL QPLIQRLPQL
210 220 230 240 250
KVVMEHITTM DAVNFVESCK EGSVGATVTP QHLLLNRNAL FQGGLQPHNY
260 270 280 290 300
CLPVLKREIH REAIVKAVTS GSKKFFLGTD SAPHERSRKE SSCGCAGIYS
310 320 330 340 350
APIALSLYAK VFDEAGALDK LEAFTSFNGP DFYGLPRNSS KITLKKSPWK
360 370
VPDVFNFPFG EIVPMFAGET LQWQPLK
Length:377
Mass (Da):41,950
Last modified:July 1, 1997 - v1
Checksum:i783C22BE5581DEDB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti179F → L in BAF00239 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000146 mRNA Translation: AAB71134.1
AL031018 Genomic DNA Translation: CAA19808.1
AL161558 Genomic DNA Translation: CAB79248.1
CP002687 Genomic DNA Translation: AEE84682.1
CP002687 Genomic DNA Translation: ANM66059.1
AK228295 mRNA Translation: BAF00239.1
PIRiT05124
RefSeqiNP_001327985.1, NM_001341566.1
NP_194024.1, NM_118422.4
UniGeneiAt.2374

Genome annotation databases

EnsemblPlantsiAT4G22930.1; AT4G22930.1; AT4G22930
AT4G22930.2; AT4G22930.2; AT4G22930
GeneIDi828392
GrameneiAT4G22930.1; AT4G22930.1; AT4G22930
AT4G22930.2; AT4G22930.2; AT4G22930
KEGGiath:AT4G22930

Similar proteinsi

Entry informationi

Entry nameiPYRC_ARATH
AccessioniPrimary (citable) accession number: O04904
Secondary accession number(s): Q0WRL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: April 25, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health