O04893 (AGLU_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alpha-glucosidase EC=3.2.1.20 Alternative name(s): Maltase
Organism	Spinacia oleracea (Spinach)
Taxonomic identifier	3562 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	903 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha-glucosidase I and II have high activity towards malto-oligosaccharides and starch, while form III and IV have high activity towards malto-oligosaccharides but low activity toward starch.
Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Post-translational modification	Four different forms (I-IV) may be produced by post-translational modification.
Sequence similarities	Belongs to the glycosyl hydrolase 31 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro maltose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 903	879	Alpha-glucosidase		PRO_0000018584
Sites
	Active site	465	1	By similarity
	Active site	468	1	By similarity
	Active site	564	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	64	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Glycosylation	400	1	N-linked (GlcNAc...) Potential
	Glycosylation	491	1	N-linked (GlcNAc...) Potential
	Glycosylation	501	1	N-linked (GlcNAc...) Potential
	Glycosylation	600	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O04893 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 5B054E27C20EC33A	FASTA	903	100,881
	10 20 30 40 50 60 MKKKIPSLAL GILLVFLLQY LVAGISTSEN DPEGVIGYGY KVKSVKVDSG TRRSLTALPQ 70 80 90 100 110 120 LVKNSSVYGP DIQLLSITAS LESNDRLRVR ITDAKHRRWE IPDNILHRHQ PPPPPPHSLS 130 140 150 160 170 180 SLYRTLLSSP TTNRRKILLS HPNSDLTFSL INTTPFGFTI SRKSTHDVLF DATPDPTNPN 190 200 210 220 230 240 TFLIFIDQYL HLTSSLPGTR AHIYGLGEHS KPTFQLAHNQ TLTMRAADIP SSNPDVNLYG 250 260 270 280 290 300 SHPFYMDVRS SPVAGSTHGV LLLNSNGMDV EYTGNRITYK VIGGIIDLYF FAGPSPGQVV 310 320 330 340 350 360 EQFTRVIGRP APMPYWAFGF QQCRYGYHDV YELQSVVAGY AKAKIPLEVM WTDIDYMDAY 370 380 390 400 410 420 KDFTLDPVNF PLDKMKKFVN NLHKNGQKYV VILDPGISTN KTYETYIRGM KHDVFLKRNG 430 440 450 460 470 480 KPYLGSVWPG PVYFPDFLKP SALTFWTDEI KRFLNLLPVD GLWIDMNEIS NFISSPPIPG 490 500 510 520 530 540 STLDNPPYKI NNSGVMLPII NKTIPPTAMH YGDIPEYNVH NLFGYLEARV TRAALIKLTE 550 560 570 580 590 600 KRPFVLSRST FSGSGKYTAH WTGDNAATWN DLVYSIPSML DFGLFGIPMV GADICGFLGN 610 620 630 640 650 660 TTEELCRRWI QLGAFYPFSR DHSSLGTTYQ ELYRWESVAA SARKVLGLRY TLLPYFYTLM 670 680 690 700 710 720 YEAQLNGIPI ARPLFFSFPD DIKTYGISSQ FLLGKGVMVS PVLKPGVVSV TAYFPRGNWF 730 740 750 760 770 780 DLFDYTRSVT ASTGRYVTLS APPDHINVHI QEGNILAMQG KAMTTQAARK TPFHLLVVMS 790 800 810 820 830 840 DCGASFGELF LDDGVEVTMG VNRGKWTFVK FIAASAKQTC IITSDVVSGE FAVSQKWVID 850 860 870 880 890 900 KVTILGLRKG TKINGYTVRT GAVTRKGDKS KLKSTPDRKG EFIVAEISGL NLLLGREFKL VLH

References

[1]	"Molecular cloning and characterization of a cDNA encoding alpha-glucosidase from spinach." Sugimoto M., Furui S., Suzuki Y. Plant Mol. Biol. 33:765-768(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Dash.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D86624 mRNA. Translation: BAA19924.1.
PIR	T09143.
3D structure databases
ProteinModelPortal	O04893.
ModBase	Search...
Protein family/group databases
CAZy	GH31. Glycoside Hydrolase Family 31.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.70. 2 hits.
InterPro	IPR013785. Aldolase_TIM. IPR011013. Gal_mutarotase_SF_dom. IPR000322. Glyco_hydro_31. IPR025887. Glyco_hydro_31_N_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR22762. PTHR22762. 1 hit.
Pfam	PF13802. Gal_mutarotas_2. 1 hit. PF01055. Glyco_hydro_31. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit. PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGLU_SPIOL

Accession

Primary (citable) accession number: O04893

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program