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O04893 (AGLU_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-glucosidase

EC=3.2.1.20
Alternative name(s):
Maltase
OrganismSpinacia oleracea (Spinach)
Taxonomic identifier3562 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Protein attributes

Sequence length903 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Alpha-glucosidase I and II have high activity towards malto-oligosaccharides and starch, while form III and IV have high activity towards malto-oligosaccharides but low activity toward starch.

Catalytic activity

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Post-translational modification

Four different forms (I-IV) may be produced by post-translational modification.

Sequence similarities

Belongs to the glycosyl hydrolase 31 family.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncarbohydrate binding

Inferred from electronic annotation. Source: InterPro

maltose alpha-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 903879Alpha-glucosidase
PRO_0000018584

Sites

Active site4651 By similarity
Active site4681 By similarity
Active site5641Proton donor By similarity

Amino acid modifications

Glycosylation641N-linked (GlcNAc...) Potential
Glycosylation2191N-linked (GlcNAc...) Potential
Glycosylation4001N-linked (GlcNAc...) Potential
Glycosylation4911N-linked (GlcNAc...) Potential
Glycosylation5011N-linked (GlcNAc...) Potential
Glycosylation6001N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O04893 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 5B054E27C20EC33A

FASTA903100,881
        10         20         30         40         50         60 
MKKKIPSLAL GILLVFLLQY LVAGISTSEN DPEGVIGYGY KVKSVKVDSG TRRSLTALPQ 

        70         80         90        100        110        120 
LVKNSSVYGP DIQLLSITAS LESNDRLRVR ITDAKHRRWE IPDNILHRHQ PPPPPPHSLS 

       130        140        150        160        170        180 
SLYRTLLSSP TTNRRKILLS HPNSDLTFSL INTTPFGFTI SRKSTHDVLF DATPDPTNPN 

       190        200        210        220        230        240 
TFLIFIDQYL HLTSSLPGTR AHIYGLGEHS KPTFQLAHNQ TLTMRAADIP SSNPDVNLYG 

       250        260        270        280        290        300 
SHPFYMDVRS SPVAGSTHGV LLLNSNGMDV EYTGNRITYK VIGGIIDLYF FAGPSPGQVV 

       310        320        330        340        350        360 
EQFTRVIGRP APMPYWAFGF QQCRYGYHDV YELQSVVAGY AKAKIPLEVM WTDIDYMDAY 

       370        380        390        400        410        420 
KDFTLDPVNF PLDKMKKFVN NLHKNGQKYV VILDPGISTN KTYETYIRGM KHDVFLKRNG 

       430        440        450        460        470        480 
KPYLGSVWPG PVYFPDFLKP SALTFWTDEI KRFLNLLPVD GLWIDMNEIS NFISSPPIPG 

       490        500        510        520        530        540 
STLDNPPYKI NNSGVMLPII NKTIPPTAMH YGDIPEYNVH NLFGYLEARV TRAALIKLTE 

       550        560        570        580        590        600 
KRPFVLSRST FSGSGKYTAH WTGDNAATWN DLVYSIPSML DFGLFGIPMV GADICGFLGN 

       610        620        630        640        650        660 
TTEELCRRWI QLGAFYPFSR DHSSLGTTYQ ELYRWESVAA SARKVLGLRY TLLPYFYTLM 

       670        680        690        700        710        720 
YEAQLNGIPI ARPLFFSFPD DIKTYGISSQ FLLGKGVMVS PVLKPGVVSV TAYFPRGNWF 

       730        740        750        760        770        780 
DLFDYTRSVT ASTGRYVTLS APPDHINVHI QEGNILAMQG KAMTTQAARK TPFHLLVVMS 

       790        800        810        820        830        840 
DCGASFGELF LDDGVEVTMG VNRGKWTFVK FIAASAKQTC IITSDVVSGE FAVSQKWVID 

       850        860        870        880        890        900 
KVTILGLRKG TKINGYTVRT GAVTRKGDKS KLKSTPDRKG EFIVAEISGL NLLLGREFKL 


VLH 

« Hide

References

[1]"Molecular cloning and characterization of a cDNA encoding alpha-glucosidase from spinach."
Sugimoto M., Furui S., Suzuki Y.
Plant Mol. Biol. 33:765-768(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. Dash.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D86624 mRNA. Translation: BAA19924.1.
PIRT09143.

3D structure databases

ProteinModelPortalO04893.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.70. 2 hits.
InterProIPR013785. Aldolase_TIM.
IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAGLU_SPIOL
AccessionPrimary (citable) accession number: O04893
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries