O04886 (PME1_CITSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pectinesterase 1
Short name=PE 1
EC=3.1.1.11

Alternative name(s):
Pectin methylesterase

Gene names

Name:

PECS-1.1

Organism

Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis)

Taxonomic identifier

2711 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Sapindales › Rutaceae › Citrus

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall Probable.
Tissue specificity	Expressed at high levels in flower buds, shoots and young leaves, and at lower levels in young fruit, young bark and juice vesicles. Not expressed at significant levels in leaf abscission zones following ethylene treatment or in mature leaves. In fruit abscission zones, expression was initially undetectable but increased markedly following ethylene treatment. Ref.1
Induction	By ethylene. Ref.1
Miscellaneous	The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.
Sequence similarities	In the N-terminal section; belongs to the PMEI family. In the C-terminal section; belongs to the pectinesterase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW enzyme inhibitor activity Inferred from electronic annotation. Source: InterPro pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 42	42	Potential
	Chain	43 – 584	542	Pectinesterase 1		PRO_0000023480
Sites
	Active site	401	1	Proton donor By similarity
	Active site	422	1	Nucleophile By similarity
	Binding site	348	1	Substrate By similarity
	Binding site	378	1	Substrate By similarity
	Binding site	490	1	Substrate By similarity
	Binding site	492	1	Substrate By similarity
	Site	400	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	108	1	N-linked (GlcNAc...) Potential
	Glycosylation	129	1	N-linked (GlcNAc...) Potential
	Glycosylation	226	1	N-linked (GlcNAc...) Potential
	Disulfide bond	415 ↔ 435		By similarity
Experimental info
	Sequence conflict	354	1	A → V in AAB57670. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O04886 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: A6E516A295092202	FASTA	584	63,513
	10 20 30 40 50 60 MTHIKEFFTK LSESSSNQNI SNIPKKKKKL FLALFATLLV VAAVIGIVAG VNSRKNSGDN 70 80 90 100 110 120 GNEPHHAILK SSCSSTRYPD LCFSAIAAVP EASKKVTSQK DVIEMSLNIT TTAVEHNYFG 130 140 150 160 170 180 IQKLLKRTNL TKREKVALHD CLETIDETLD ELHKAVEDLE EYPNKKSLSQ HADDLKTLMS 190 200 210 220 230 240 AAMTNQGTCL DGFSHDDANK HVRDALSDGQ VHVEKMCSNA LAMIKNMTDT DMMIMRTSNN 250 260 270 280 290 300 RKLTEETSTV DGWPAWLSPG DRRLLQSSSV TPNAVVAADG SGNFKTVAAA VAAAPQGGTK 310 320 330 340 350 360 RYIIRIKAGV YRENVEVTKK HKNIMFIGDG RTRTIITGSR NVVDGSTTFK SATAAVVGEG 370 380 390 400 410 420 FLARDITFQN TAGPSKHQAV ALRVGADLSA FYNCDMLAYQ DTLYVHSNRQ FFVNCLIAGT 430 440 450 460 470 480 VDFIFGNAAA VLQNCDIHAR KPNSGQKNMV TAQGRTDPNQ NTGIVIQKSR IGATSDLKPV 490 500 510 520 530 540 QGSFPTYLGR PWKEYSRTVI MQSSITDLIH PAGWHEWDGN FALNTLFYGE HQNSGAGAGT 550 560 570 580 SGRVKWKGFR VITSATEAQA FTPGSFIAGS SWLGSTGFPF SLGL

References

[1]	"Genetics and expression of two pectinesterase genes in Valencia orange." Nairn C.J., Lewandowski D.J., Burns J.K. Physiol. Plantarum 102:226-235(1998) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Valencia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82973 Genomic DNA. Translation: AAB57667.1. U82976 mRNA. Translation: AAB57670.1.
PIR	T10485.
3D structure databases
ProteinModelPortal	O04886.
SMR	O04886. Positions 266-584.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. IPR006501. Pectinesterase_inhib. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit. G3DSA:1.20.140.40. Pectinesterase_inhib. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. PF04043. PMEI. 1 hit. [Graphical view]
SMART	SM00856. PMEI. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit. SSF101148. Pectinesterase_inhib. 1 hit.
TIGRFAMs	TIGR01614. PME_inhib. 1 hit.
PROSITE	PS00800. PECTINESTERASE_1. 1 hit. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME1_CITSI

Accession

Primary (citable) accession number: O04886
Secondary accession number(s): O04888

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 1, 1997
Last modified:	November 16, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program