Reviewed, UniProtKB/Swiss-Prot O04883 (PLDA1_PIMBR)

Names and origin

Protein names

Recommended name:
    Phospholipase D alpha 1
      Short name=PLD alpha 1
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 1
    Phosphatidylcholine-hydrolyzing phospholipase D 1

Gene names

Name:	PLD1
Synonyms:	PLD

Organism

Pimpinella brachycarpa

Taxonomic identifier

45043 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Apiales › Apiaceae › Apioideae › apioid superclade › Pimpinella clade › Pimpinella

Protein attributes

Sequence length	808 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Cofactor	Calcium By similarity.
Domain	C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.
Sequence similarities	Belongs to the phospholipase D family. C2-PLD subfamily. Contains 1 C2 domain. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Repeat
Ligand	Calcium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylcholine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 808	808	Phospholipase D alpha 1		PRO_0000218822
Regions
	Domain	1 – 109	109	C2
	Domain	326 – 364	39	PLD phosphodiesterase 1
	Domain	654 – 681	28	PLD phosphodiesterase 2
Sites
	Active site	331	1	Potential
	Active site	333	1	Potential
	Active site	338	1	Potential
	Active site	659	1	Potential
	Active site	661	1	Potential
	Active site	666	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	O04883-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: E83DA015B06F2164	FASTA	808	91,673
	10 20 30 40 50 60 MAKTLLHGTL HVTIFEVDHL KAGSVVVFSE SLRRTLRKPL VLAKGTPKIY ASIDLDKARV 70 80 90 100 110 120 GRTRMIENEP NNPKWNESFH IYCGHPSTNV IFTVKDDNPI GATLIGRAYL PVHELLEGEE 130 140 150 160 170 180 VDKWVEILDE DKNPISEGSK IHVKLQYFDI TQDRNWAHGI RSSKFPGVPY TFFSQRPGCR 190 200 210 220 230 240 ISLYQDAHVP DNFVPKIPLS GGKFYEPHRC WEDVFDAITN AKHFIYITGW SVYTEFALIR 250 260 270 280 290 300 DTRRPKPGGD IMLGELLKKK ADEGVRVLML VWDDRTSVGL LKKDGLMATH DQETEEYFRD 310 320 330 340 350 360 SNVHCVLCLR NPDDGGGIIQ GLTISTIFTH HQKIVVVDSE MPTSGSENRR VVSFVGGIDL 370 380 390 400 410 420 CDGRYDTPFH SLFRTLDTAH HDDFHQPNFE GAAITKGGPR EPWHDIHSRL EGPVAWDVLF 430 440 450 460 470 480 NFEQRWRKQG GKDILLNLRE LQDVIIPPSP VTFPDDDETW NVQLFRSIDE GAAFFFPQTP 490 500 510 520 530 540 EEAAKAGLVS GKENIIVRSI QDAYIHAIRG PKISFILKIS IFLEALLAGI QRILKMRTSV 550 560 570 580 590 600 LCILIPKELS LKIVSKIEAG KRFTVYVVLP MWPEGIPESG SVQAILDWQR RTMEMMYKDI 610 620 630 640 650 660 IQALQANGIE EDPRNYLTFF CLGNREVKRD GEYEPSEKPD PDTDYSRAQE SRRFMIYVHA 670 680 690 700 710 720 KMMIVDDEYI IIGSANINQR SMDGAKDSEI AMGAYQPHHL ATREPARGQI HGFRMSLWYE 730 740 750 760 770 780 HLGMLDDTLA LPESVDCVQK VNTVADKYWD LYSSETLEND LPGHLLRYPI AVASEGNVTE 790 800 LPGTEFFPDT KARVLGAKSD FLPPILTT

References

[1]	"Nucleotide sequence of a cDNA encoding phospholipase D from Pimpinella brachycarpa." Cha Y.Y., Lee K.-W., Kim J.C., Han T.J., Lee W.S., Cho S.H. Plant Gene Register PGR97-092 Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U96438 mRNA. Translation: AAB70463.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.4.4. 96847.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR015679. Phospholipase_D. IPR011402. PLD_pln. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00168. C2. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
PIRSF	PIRSF036470. PLD_plant. 1 hit.
SMART	SM00239. C2. 1 hit. SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDA1_PIMBR

Accession

Primary (citable) accession number: O04883

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)