Reviewed, UniProtKB/Swiss-Prot O04866 (ARGD_ALNGL)

Names and origin

Protein names

Recommended name:
    Acetylornithine aminotransferase, mitochondrial
      Short name=ACOAT
    EC=2.6.1.11
Alternative name(s):
    Acetylornithine transaminase
      Short name=AOTA

Gene names

Name:

AG118

Organism

Alnus glutinosa (Alder)

Taxonomic identifier

3517 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fagales › Betulaceae › Alnus

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the biosynthesis of citrulline.
Catalytic activity	N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.
Subcellular location	Mitochondrion.
Tissue specificity	Found at highest levels in nodules, confined to the infected cells.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 451		Acetylornithine aminotransferase, mitochondrial		PRO_0000002075
Amino acid modifications
	Modified residue	302	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O04866-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: B8DFC693F0A3B196	FASTA	451	48,832
	10 20 30 40 50 60 MTSLQYFSLN RPVFPATHLH RPGIRHLQVS ACANVEVQAP SSVKKQGVSK EVMEAAGRVL 70 80 90 100 110 120 VGTYARVPVV LSRGKGCKLY DPEGREYLDL SAGIAVNVLG HADSDWLRAV TEQAATLTHV 130 140 150 160 170 180 SNVFYSIPQV ELAKRLVASS FADRVFFSNS GTEANEAAIK FARKFQRFTR PDEKQPATEF 190 200 210 220 230 240 VSFSNSFHGR TMGSLALTSK ENYRSPFEPV MPGVTFLEYG NIEAATQLIQ RRKIAAVFVE 250 260 270 280 290 300 PIQGEGGVYS ATKEFLYALR KACDDSGTLL VFDEVQCGLG RTGYLWAHEI YDVFPDIMTL 310 320 330 340 350 360 AKPLAGGLPI GAVLVTERVA SAITYGDHGT TFAGGPLVCK AALTVLDKIL RPGFLASVSK 370 380 390 400 410 420 KGHYFKEMLI NKLGGNSHVR EVRGVGLIVG IELDVSASPL VNACLNSGLL VLTAGKGNVV 430 440 450 RIVPPLIITE QELEKAAEIL LQCLPALDRH G

References

[1]

"Nitrogen metabolism in actinorhizal nodules of Alnus glutinosa: expression of glutamine synthetase and acetylornithine transaminase." Guan C., Ribeiro A., Akkermans A.D.L., Jing Y., van Kammen A., Bisseling T., Pawlowski K. Plant Mol. Biol. 32:1177-1184(1996) [PubMed: 9002618] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Root nodule.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y08680 mRNA. Translation: CAA69936.1.
3D structure databases
HSSP	HSSP built from PDB template 1QJ3 based on UniProtKB P12995.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.6.1.11. 262895.
Family and domain databases
InterPro	IPR004636. AcOrn/succinylOrn_aminoTrfase. IPR005814. Aminotrans_3. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF19. ArgD_aminotrans. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00707. argD. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGD_ALNGL

Accession

Primary (citable) accession number: O04866

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 1, 1997
Last modified:	September 22, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)