O04865 (PLDA1_VIGUN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholipase D alpha 1
Short name=PLD alpha 1
EC=3.1.4.4

Alternative name(s):
Choline phosphatase 1
Phosphatidylcholine-hydrolyzing phospholipase D 1

Gene names

Name:

PLD1

Organism

Vigna unguiculata (Cowpea)

Taxonomic identifier

3917 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length	809 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Cofactor	Calcium By similarity.
Domain	C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.
Sequence similarities	Belongs to the phospholipase D family. C2-PLD subfamily. Contains 1 C2 domain. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Repeat
Ligand	Calcium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylcholine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 809	809	Phospholipase D alpha 1		PRO_0000218824
Regions
	Domain	1 – 109	109	C2
	Domain	326 – 365	40	PLD phosphodiesterase 1
	Domain	655 – 682	28	PLD phosphodiesterase 2
Sites
	Active site	331	1	Potential
	Active site	333	1	Potential
	Active site	338	1	Potential
	Active site	660	1	Potential
	Active site	662	1	Potential
	Active site	667	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	O04865 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 4EFE00B634DDD946	FASTA	809	91,565
	10 20 30 40 50 60 MAQILLHGTL HATIYEVDEL HGGGGGNFFS KLKQNIEETV GIGKGVTKLY ATIDLEKARV 70 80 90 100 110 120 GRTRIIENET TNPKWNESFH IYCGHLASNI IFTVKDDNPI GATLIGRAYV PVSEVLDGHE 130 140 150 160 170 180 IDKWVEILDT EKNPIEGGSK IHVRLQYFDV LKDRNWARGI RSPKYPGVPY TFFSQRQGCK 190 200 210 220 230 240 VFLYQDAHVP DNFVPKIPLA GGKNYEAHRC WEDIFDAITN AKHLIYITGW SVYTEISLIR 250 260 270 280 290 300 DSRRPKAGGD QTIGELLKKK ASEGVRVLML VWDDRTSVGL LKKDGLMATH DEETEQFFRD 310 320 330 340 350 360 TDVHCVLCPR NPDDGGSIVQ DLQISTMFTH HQKIVVVDSA LPGGGGSDKR RIVSFVGGLD 370 380 390 400 410 420 LCDGRYDTAF HSLFRTLDTA HHDDFHQPNF PGAAITKGGP REPWHDIHSR VEGPIAWDVL 430 440 450 460 470 480 FNFEQRWRKQ GGKDILAPLR ELEDVIIPPS PVTFPDDHET WNVQLFRSID GGAAFGFPDT 490 500 510 520 530 540 PEDAAKAGLV SGKDNIIDRS IQDAYIHAIR RAKNFIYIEN QYFLGSSFSW NNDDIKREEI 550 560 570 580 590 600 GALHLIPKEL SLKIVSKIEA GERFAVYVVV PMWPEGIPES SSVQAILDWQ KRTIEMMYKD 610 620 630 640 650 660 VVQALRAKGS DEDPRNYLTF FCLGNREVKK SGEYEPAEQP EPDSDYQRAQ EARRFMIYVH 670 680 690 700 710 720 TKMMIVDDEY IIIGSANINQ RSMDGARDSE IAMGGYQPYH LANTQPARGQ VYGFRMSLWY 730 740 750 760 770 780 EHLGMLHDTF QRPESEECIN KVNQIADKYW DLYSSESLER DLPGHLLRYP IGVASEGEVT 790 800 ELPGFEFFPD TKARILGAKA DYLPPILTT

References

[1]	el Maarouf H., Pham Thi A.T., Gareil M., D'Arcy-Lameta A., Zuily-Fodil Y. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Epace-1. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U92656 mRNA. Translation: AAB51392.1.
PIR	T11695.
3D structure databases
ProteinModelPortal	O04865.
ModBase	Search...
Proteomic databases
PRIDE	O04865.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR015679. PLipase_D. IPR001736. PLipase_D/transphosphatidylase. IPR024632. PLipase_D_C. IPR011402. PLipase_D_pln. [Graphical view]
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00168. C2. 1 hit. PF12357. PLD_C. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
PIRSF	PIRSF036470. PLD_plant. 1 hit.
SMART	SM00239. C2. 1 hit. SM00155. PLDc. 2 hits. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit.
PROSITE	PS50004. C2. False negative. PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDA1_VIGUN

Accession

Primary (citable) accession number: O04865

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	October 19, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program