O04857 (PP12_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 2
EC=3.1.3.16

Gene names

Name:

NPP2

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Serine/threonine-protein phosphatase PP1 isozyme 2		PRO_0000058812
Sites
	Active site	129	1	Proton donor By similarity
	Metal binding	68	1	Iron By similarity
	Metal binding	70	1	Iron By similarity
	Metal binding	96	1	Iron By similarity
	Metal binding	96	1	Manganese By similarity
	Metal binding	128	1	Manganese By similarity
	Metal binding	177	1	Manganese By similarity
	Metal binding	252	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	O04857 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 4EFA9FB525B7D4C3	FASTA	310	35,435
	10 20 30 40 50 60 MAQNGQGIEP AVLDDIINRL LEFRNARTVR QVQLSEAEIR SLCTASKEIF LHQPNLLELE 70 80 90 100 110 120 APIKICGDIH GQYGDLLRLF EYGGFPPEAN YLFLGDYVDR GKQSLETICL LLAYKIKYPE 130 140 150 160 170 180 NFFLLRGNHE CASINRIYGF YDECKRRFNV RLWKTNTDCF NCLPVAALID DKILCMHGGL 190 200 210 220 230 240 SPDLTDLDEI RNLPRPTDIP DSGLLCDLLW SDPSREVKGW GMNDRGVSYT FGSDKVAEFL 250 260 270 280 290 300 MQHDMDLVCR AHQVVEDGYE FFAERQLVTI FSAPNYCGEF DNAGAMMSVD ENLMCSFQIL 310 KPTDRKPRFL

References

[1]	"Multiple genes encoding serine/threonine protein phosphatases and their differential expression in Nicotiana tabacum." Suh M., Cho H., Kim Y., Liu J., Lee H. Plant Mol. Biol. 36:315-322(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Xanthi.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z93769 mRNA. Translation: CAB07804.1.
PIR	T03596.
3D structure databases
ProteinModelPortal	O04857.
SMR	O04857. Positions 13-309.
ModBase	Search...
Proteomic databases
PRIDE	O04857.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP12_TOBAC

Accession

Primary (citable) accession number: O04857

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program