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Protein

Deacetoxyvindoline 4-hydroxylase

Gene

D4H

Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the C4-hydroxylation of desacetoxyvindoline.

Catalytic activityi

Deacetoxyvindoline + 2-oxoglutarate + O2 = deacetylvindoline + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: vindoline biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes vindoline from tabersonine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Tabersonine 16-hydroxylase (CYP71D12)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Deacetoxyvindoline 4-hydroxylase (D4H)
  6. Deacetylvindoline O-acetyltransferase (DAT)
This subpathway is part of the pathway vindoline biosynthesis, which is itself part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes vindoline from tabersonine, the pathway vindoline biosynthesis and in Alkaloid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi268IronPROSITE-ProRule annotation1
Metal bindingi324IronPROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

BRENDAi1.14.11.20. 1211.
UniPathwayiUPA00365; UER00525.

Names & Taxonomyi

Protein namesi
Recommended name:
Deacetoxyvindoline 4-hydroxylase (EC:1.14.11.20)
Gene namesi
Name:D4H
OrganismiCatharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Taxonomic identifieri4058 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeCatharanthinaeCatharanthus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000672821 – 401Deacetoxyvindoline 4-hydroxylaseAdd BLAST401

Expressioni

Tissue specificityi

Highest levels in leaves, lower levels in stems and fruits. Not expressed in flowers and roots.

Inductioni

By light.1 Publication

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliO04847.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini242 – 345Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST104

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK12697.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O04847-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKSWPIVIS SHSFCFLPNS EQERKMKDLN FHAATLSEEE SLRELKAFDE
60 70 80 90 100
TKAGVKGIVD TGITKIPRIF IDQPKNLDRI SVCRGKSDIK IPVINLNGLS
110 120 130 140 150
SNSEIRREIV EKIGEASEKY GFFQIVNHGI PQDVMDKMVD GVRKFHEQDD
160 170 180 190 200
QIKRQYYSRD RFNKNFLYSS NYVLIPGIAC NWRDTMECIM NSNQPDPQEF
210 220 230 240 250
PDVCRDILMK YSNYVRNLGL ILFELLSEAL GLKPNHLEEM DCAEGLILLG
260 270 280 290 300
HYYPACPQPE LTFGTSKHSD SGFLTILMQD QIGGLQILLE NQWIDVPFIP
310 320 330 340 350
GALVINIADL LQLITNDKFK SVEHRVLANK VGPRISVAVA FGIKTQTQEG
360 370 380 390 400
VSPRLYGPIK ELISEENPPI YKEVTVKDFI TIRFAKRFDD SSSLSPFRLN

N
Length:401
Mass (Da):45,647
Last modified:November 1, 1998 - v2
Checksum:i95B66916E01C8979
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136D → A AA sequence (PubMed:9290645).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008597 Genomic DNA. Translation: AAB97311.1.
U71604 mRNA. Translation: AAC49826.1.
U71605 mRNA. Translation: AAC49827.1.
PIRiT07914.

Genome annotation databases

KEGGiag:AAB97311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008597 Genomic DNA. Translation: AAB97311.1.
U71604 mRNA. Translation: AAC49826.1.
U71605 mRNA. Translation: AAC49827.1.
PIRiT07914.

3D structure databases

ProteinModelPortaliO04847.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAB97311.

Phylogenomic databases

KOiK12697.

Enzyme and pathway databases

UniPathwayiUPA00365; UER00525.
BRENDAi1.14.11.20. 1211.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDV4H_CATRO
AccessioniPrimary (citable) accession number: O04847
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 1, 1998
Last modified: November 11, 2015
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.