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Protein

Neutral peroxidase

Gene
N/A
Organism
Ipomoea batatas (Sweet potato) (Convolvulus batatas)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
May contribute to protection against cold-induced oxidative stress.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+Note: Binds 2 calcium ions per subunit.
  • heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei96Transition state stabilizerPROSITE-ProRule annotation1
Active sitei100Proton acceptorPROSITE-ProRule annotation1
Metal bindingi101Calcium 1PROSITE-ProRule annotation1
Metal bindingi104Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi106Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi108Calcium 1PROSITE-ProRule annotation1
Binding sitei198Substrate; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi228Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi229Calcium 2PROSITE-ProRule annotation1
Metal bindingi269Calcium 2PROSITE-ProRule annotation1
Metal bindingi271Calcium 2PROSITE-ProRule annotation1
Metal bindingi276Calcium 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Protein family/group databases

PeroxiBasei65. IbPrx09.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral peroxidase (EC:1.11.1.7)
Alternative name(s):
SwPN1
OrganismiIpomoea batatas (Sweet potato) (Convolvulus batatas)
Taxonomic identifieri4120 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesConvolvulaceaeIpomoeeaeIpomoea

Subcellular locationi

  • Secreted PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000002375121 – 671 PublicationAdd BLAST47
ChainiPRO_000002375268 – 348Neutral peroxidaseAdd BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi71 ↔ 149PROSITE-ProRule annotation
Disulfide bondi102 ↔ 107PROSITE-ProRule annotation
Glycosylationi114N-linked (GlcNAc...)Sequence analysis1
Glycosylationi118N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi156 ↔ 344PROSITE-ProRule annotation
Glycosylationi173N-linked (GlcNAc...)Sequence analysis1
Glycosylationi177N-linked (GlcNAc...)Sequence analysis1
Glycosylationi189N-linked (GlcNAc...)Sequence analysis1
Glycosylationi203N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi235 ↔ 256PROSITE-ProRule annotation
Glycosylationi247N-linked (GlcNAc...)Sequence analysis1
Glycosylationi261N-linked (GlcNAc...)Sequence analysis1
Glycosylationi300N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Highly expressed in suspension cultured cells. Weak expression also found in the stems of intact plants. No expression in leaf, tuberous root and non-tuberous root.

Developmental stagei

Very low expression level 0.5 days after subculture (DAS).

Inductioni

By wounding and cold stress. Induced by acclimation and repressed by chilling.

Structurei

3D structure databases

ProteinModelPortaliO04796.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

CDDicd00693. secretory_peroxidase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
IPR033905. Secretory_peroxidase.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04796-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFVARLTL ALSFIALALA GYSLVQNTLS SPTHTRLNLI PTWLDSTFDS
60 70 80 90 100
ADVLSYLGFG KSSGRLSDSN CVFSAVKEIV DAAITAETRM GASLIRLHFH
110 120 130 140 150
DCFVDGCDGG ILLNDTANFT GEQGAPANSN SVRGFSVIDQ AKRNAQTKCA
160 170 180 190 200
DTPVSCADVL AIAARDAFRK FTNQTYNITL GRQDARTANL TGANTQLPAP
210 220 230 240 250
FDNLSIQTAK FADKGFNQRE MVVLAGAHTV GFSRCAVLCT STNLNQNRSA
260 270 280 290 300
TLQCTCPASA NDTGLVGLDP SPGTFDKKYF EELVKGQGLL FSDQELMQSN
310 320 330 340
ATVTAVRRYR DATGAFLTDF AAAMVKMSNL PPSAGVQLEI RNVCSRVN
Length:348
Mass (Da):37,186
Last modified:July 1, 1997 - v1
Checksum:i3FA8C361B72CD383
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z84473 mRNA. Translation: CAB06478.1.
PIRiT10946.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z84473 mRNA. Translation: CAB06478.1.
PIRiT10946.

3D structure databases

ProteinModelPortaliO04796.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

PeroxiBasei65. IbPrx09.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd00693. secretory_peroxidase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
IPR033905. Secretory_peroxidase.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPERN_IPOBA
AccessioniPrimary (citable) accession number: O04796
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 4, 2003
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.