ID   C75A7_EUSGR             Reviewed;         510 AA.
AC   O04790;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   24-MAR-2009, entry version 52.
DE   RecName: Full=Flavonoid 3',5'-hydroxylase;
DE            Short=F3'5'H;
DE            EC=1.14.13.88;
DE   AltName: Full=Cytochrome P450 75A7;
GN   Name=CYP75A7;
OS   Eustoma grandiflorum (Bluebells) (Lisianthus russellianus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Gentianales; Gentianaceae; Chironieae; Eustoma.
OX   NCBI_TaxID=52518;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Royal light purple; TISSUE=Petal;
RA   Ohbayashi M.;
RL   Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Petal;
RA   Noda N., Kanno Y., Kato N., Kazuma K., Suzuki M.;
RT   "Regulation of gene expression involved in flavonol and anthocyanin
RT   biosynthesis during petal development in lisianthus (Eustoma
RT   grandiflorum).";
RL   Physiol. Plantarum 122:305-313(2004).
CC   -!- FUNCTION: Catalyzes the 3'5'-hydroxylation of naringenin and
CC       eriodictyol to form 5,7,3,'4',5'-pentahydroxyflavanone and 3',5'-
CC       hydroxylation of dihydrokaempferol and dihydroquercetin to form
CC       dihydromyricetin (By similarity).
CC   -!- CATALYTIC ACTIVITY: A flavanone + NADPH + O(2) = a 3'-
CC       hydroxyflavanone + NADP(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: A 3'-hydroxyflavanone + NADPH + O(2) = a
CC       3',5'-dihydroxyflavanone + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; D14589; BAA03439.1; -; mRNA.
DR   EMBL; AB078957; BAD34460.1; -; mRNA.
DR   BRENDA; 1.14.13.88; 276991.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0033772; F:flavonoid 3',5'-hydroxylase activity; IEA:EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase.
FT   CHAIN         1    510       Flavonoid 3',5'-hydroxylase.
FT                                /FTId=PRO_0000052134.
FT   METAL       447    447       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   510 AA;  56732 MW;  B0A84B8F2DB2771D CRC64;
     MAVGNGVLLH IAASLMLFFH VQKLVQYLWM NSRRHRLPPG PIGWPVLGAL RLLGTMPHVA
     LANMAKKYGP VMYLKVGSCG LAVASTPEAA KAFLKTLDMN FSNRPPNAGA THLAYNAQDM
     VFADYGPRWK LLRKLSNIHI LGGKALQGWE EVRKKELGYM LYAMAESGRH GQPVVVSEML
     TYAMANMLGQ VMLSKRVFGS QGSESNEFKD MVVELMTVAG YFNIGDFIPS IAWMDLQGIQ
     GGMKRLHKKF DALLTRLLEE HTASAHERKG SPDFLDFVVA NGDNSEGERL QTVNIKALLL
     NMFTAGTDTS SSVIEWALAE LLKNPIILRR AQEEMDGVIG RDRRFLEADI SKLPYLQAIC
     KEAFRKHPST PLNLPRIASQ ACEVNGHYIP KGTRLSVNIW AIGRDPSVWE NPNEFNPDRF
     LERKNAKIDP RGNDFELIPF GAGRRICAGT RLGILLVEYI LGTLVHSFVW ELPSSVIELN
     MDESFGLALQ KAVPLAAMVT PRLPLHIYSP
//