O04790 (C75A7_EUSGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flavonoid 3',5'-hydroxylase
Short name=F3'5'H
EC=1.14.13.88

Alternative name(s):
Cytochrome P450 75A7

Gene names

Name:

CYP75A7

Organism

Eustoma grandiflorum (Bluebells) (Lisianthus russellianus)

Taxonomic identifier

52518 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Gentianaceae › Chironieae › Eustoma

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the 3'5'-hydroxylation of naringenin and eriodictyol to form 5,7,3,'4',5'-pentahydroxyflavanone and 3',5'-hydroxylation of dihydrokaempferol and dihydroquercetin to form dihydromyricetin By similarity.
Catalytic activity	A flavanone + NADPH + O2 = a 3'-hydroxyflavanone + NADP+ + H2O. A 3'-hydroxyflavanone + NADPH + O2 = a 3',5'-dihydroxyflavanone + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Pigment biosynthesis; anthocyanin biosynthesis.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro flavonoid 3',5'-hydroxylase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Flavonoid 3',5'-hydroxylase		PRO_0000052134
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O04790 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: B0A84B8F2DB2771D	FASTA	510	56,732
	10 20 30 40 50 60 MAVGNGVLLH IAASLMLFFH VQKLVQYLWM NSRRHRLPPG PIGWPVLGAL RLLGTMPHVA 70 80 90 100 110 120 LANMAKKYGP VMYLKVGSCG LAVASTPEAA KAFLKTLDMN FSNRPPNAGA THLAYNAQDM 130 140 150 160 170 180 VFADYGPRWK LLRKLSNIHI LGGKALQGWE EVRKKELGYM LYAMAESGRH GQPVVVSEML 190 200 210 220 230 240 TYAMANMLGQ VMLSKRVFGS QGSESNEFKD MVVELMTVAG YFNIGDFIPS IAWMDLQGIQ 250 260 270 280 290 300 GGMKRLHKKF DALLTRLLEE HTASAHERKG SPDFLDFVVA NGDNSEGERL QTVNIKALLL 310 320 330 340 350 360 NMFTAGTDTS SSVIEWALAE LLKNPIILRR AQEEMDGVIG RDRRFLEADI SKLPYLQAIC 370 380 390 400 410 420 KEAFRKHPST PLNLPRIASQ ACEVNGHYIP KGTRLSVNIW AIGRDPSVWE NPNEFNPDRF 430 440 450 460 470 480 LERKNAKIDP RGNDFELIPF GAGRRICAGT RLGILLVEYI LGTLVHSFVW ELPSSVIELN 490 500 510 MDESFGLALQ KAVPLAAMVT PRLPLHIYSP

References

[1]	Ohbayashi M. Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Royal light purple. Tissue: Petal.
[2]	"Regulation of gene expression involved in flavonol and anthocyanin biosynthesis during petal development in lisianthus (Eustoma grandiflorum)." Noda N., Kanno Y., Kato N., Kazuma K., Suzuki M. Physiol. Plantarum 122:305-313(2004) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Petal.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14589 mRNA. Translation: BAA03439.1. AB078957 mRNA. Translation: BAD34460.1.
3D structure databases
ProteinModelPortal	O04790.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.88. 7339.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C75A7_EUSGR

Accession

Primary (citable) accession number: O04790

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	September 21, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program