ID   C75A6_CAMME             Reviewed;         523 AA.
AC   O04773;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   24-MAR-2009, entry version 51.
DE   RecName: Full=Flavonoid 3',5'-hydroxylase;
DE            Short=F3'5'H;
DE            EC=1.14.13.88;
DE   AltName: Full=Cytochrome P450 75A6;
GN   Name=CYP75A6;
OS   Campanula medium (Canterbury bells).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; campanulids; Asterales; Campanulaceae; Campanula.
OX   NCBI_TaxID=56154;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Petal;
RA   Ohbayashi M.;
RL   Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the 3'5'-hydroxylation of naringenin and
CC       eriodictyol to form 5,7,3,'4',5'-pentahydroxyflavanone and 3',5'-
CC       hydroxylation of dihydrokaempferol and dihydroquercetin to form
CC       dihydromyricetin (By similarity).
CC   -!- CATALYTIC ACTIVITY: A flavanone + NADPH + O(2) = a 3'-
CC       hydroxyflavanone + NADP(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: A 3'-hydroxyflavanone + NADPH + O(2) = a
CC       3',5'-dihydroxyflavanone + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; D14590; BAA03440.1; -; mRNA.
DR   BRENDA; 1.14.13.88; 294891.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0033772; F:flavonoid 3',5'-hydroxylase activity; IEA:EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase.
FT   CHAIN         1    523       Flavonoid 3',5'-hydroxylase.
FT                                /FTId=PRO_0000052135.
FT   METAL       460    460       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   523 AA;  58726 MW;  70E39C6B76F387D9 CRC64;
     MSIDISTLFY ELVAAISLYL ATYSFIRFLF KPSHHHHLPP GPTGWPIIGA LPLLGTMPHV
     SLADMAVKYG PIMYLKLGSK GTVVASNPKA ARAFLKTHDA NFSNRPIDGG PTYLAYNAQD
     MVFAEYGPKW KLLRKLCSLH MLGPKALEDW AHVKVSEVGH MLKEMYEQSS KSVPVPVVVP
     EMLTYAMANM IGRIILSRRP FVITSKLDSS ASASASVSEF QYMVMELMRM AGLFNIGDFI
     PYIAWMDLQG IQRDMKVIQK KFDVLLNKMI KEHTESAHDR KDNPDFLDIL MAATQENTEG
     IQLNLVNVKA LLLDLFTAGT DTSSSVIEWA LAEMLNHRQI LNRAHEEMDQ VIGRNRRLEQ
     SDIPNLPYFQ AICKETFRKH PSTPLNLPRI STEACEVDGF HIPKNTRLIV NIWAIGRDPK
     VWENPLDFTP ERFLSEKHAK IDPRGNHFEL IPFGAGRRIC AGARMGAASV EYILGTLVHS
     FDWKLPDGVV EVNMEESFGI ALQKKVPLSA IVTPRLPPSS YTV
//