O04773 (C75A6_CAMME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flavonoid 3',5'-hydroxylase
Short name=F3'5'H
EC=1.14.13.88

Alternative name(s):
Cytochrome P450 75A6

Gene names

Name:

CYP75A6

Organism

Campanula medium (Canterbury bells)

Taxonomic identifier

56154 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Campanulaceae › Campanula

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the 3'5'-hydroxylation of naringenin and eriodictyol to form 5,7,3,'4',5'-pentahydroxyflavanone and 3',5'-hydroxylation of dihydrokaempferol and dihydroquercetin to form dihydromyricetin By similarity.
Catalytic activity	A flavanone + NADPH + O2 = a 3'-hydroxyflavanone + NADP+ + H2O. A 3'-hydroxyflavanone + NADPH + O2 = a 3',5'-dihydroxyflavanone + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Pigment biosynthesis; anthocyanin biosynthesis.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro flavonoid 3',5'-hydroxylase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 523	523	Flavonoid 3',5'-hydroxylase		PRO_0000052135
Sites
	Metal binding	460	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O04773 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 70E39C6B76F387D9	FASTA	523	58,726
	10 20 30 40 50 60 MSIDISTLFY ELVAAISLYL ATYSFIRFLF KPSHHHHLPP GPTGWPIIGA LPLLGTMPHV 70 80 90 100 110 120 SLADMAVKYG PIMYLKLGSK GTVVASNPKA ARAFLKTHDA NFSNRPIDGG PTYLAYNAQD 130 140 150 160 170 180 MVFAEYGPKW KLLRKLCSLH MLGPKALEDW AHVKVSEVGH MLKEMYEQSS KSVPVPVVVP 190 200 210 220 230 240 EMLTYAMANM IGRIILSRRP FVITSKLDSS ASASASVSEF QYMVMELMRM AGLFNIGDFI 250 260 270 280 290 300 PYIAWMDLQG IQRDMKVIQK KFDVLLNKMI KEHTESAHDR KDNPDFLDIL MAATQENTEG 310 320 330 340 350 360 IQLNLVNVKA LLLDLFTAGT DTSSSVIEWA LAEMLNHRQI LNRAHEEMDQ VIGRNRRLEQ 370 380 390 400 410 420 SDIPNLPYFQ AICKETFRKH PSTPLNLPRI STEACEVDGF HIPKNTRLIV NIWAIGRDPK 430 440 450 460 470 480 VWENPLDFTP ERFLSEKHAK IDPRGNHFEL IPFGAGRRIC AGARMGAASV EYILGTLVHS 490 500 510 520 FDWKLPDGVV EVNMEESFGI ALQKKVPLSA IVTPRLPPSS YTV

References

[1]	Ohbayashi M. Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Petal.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14590 mRNA. Translation: BAA03440.1.
3D structure databases
ProteinModelPortal	O04773.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C75A6_CAMME

Accession

Primary (citable) accession number: O04773

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	September 21, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program