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Protein

Protein phosphatase 2C 77

Gene

ABI2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane (Pos), high light stress, response to glucose, seed germination and inhibition of vegetative growth. During the stomatal closure regulation, modulates the inward calcium-channel permeability as well as H2O2 and oxidative burst in response to ABA and dehydration. Controls negatively fibrillin that is involved in mediating ABA-induced photoprotection. May be implicated in ABA content regulation. Involved in acquired thermotolerance of root growth and seedling survival. Required for the Erwinia amylovora harpin-induced (HrpN) drought tolerance. Involved in the hydrotropic response.23 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+3 Publications, Mn2+1 PublicationNote: Binds 2 magnesium or manganese ions per subunit.3 Publications

Enzyme regulationi

Phosphatase activity repressed by oxidized ATGPX3, free fatty acids (e.g. arachidonic acid (20:4) and Linolenic acid (18:3)) and by H2O2. Repressed by PYR/PYL/RCAR ABA receptors in an ABA-dependent manner.3 Publications

pH dependencei

Optimum pH is 8.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi165 – 1651Magnesium or manganese 1Combined sources
Metal bindingi165 – 1651Magnesium or manganese 2Combined sources
Metal bindingi166 – 1661Magnesium or manganese 1; via carbonyl oxygenBy similarity
Metal bindingi251 – 2511Magnesium or manganese 1Combined sources
Metal bindingi252 – 2521Magnesium or manganese 1Combined sources
Sitei290 – 2901LockBy similarity
Metal bindingi337 – 3371Magnesium or manganese 1; via amide nitrogenCombined sources
Metal bindingi337 – 3371Magnesium or manganese 2Combined sources
Metal bindingi341 – 3411Magnesium or manganese 1By similarity
Metal bindingi402 – 4021Magnesium or manganese 2Combined sources

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • negative regulation of protein kinase activity Source: TAIR
  • photoinhibition Source: TAIR
  • regulation of stomatal opening Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to heat Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Abscisic acid signaling pathway

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G57050-MONOMER.
ARA:GQT-1437-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 77 (EC:3.1.3.16)
Short name:
AtPP2C77
Alternative name(s):
Protein ABSCISIC ACID-INSENSITIVE 2
Protein phosphatase 2C ABI2
Short name:
PP2C ABI2
Gene namesi
Name:ABI2
Ordered Locus Names:At5g57050
ORF Names:MHM17.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G57050.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi168 – 1681G → D in abi2; reduced phosphatase activity, reduced affinity with magnesium ions, loss of interaction with the fibrillin precursor protein, impaired ABA-mediated binding to PYR1, and reduced negative control on fibrillin activity. 3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Protein phosphatase 2C 77PRO_0000057767Add
BLAST

Proteomic databases

PaxDbiO04719.
PRIDEiO04719.

PTM databases

iPTMnetiO04719.

Expressioni

Inductioni

Repressed by MYB44 and ERF4. Induced by salt stress and ABA.4 Publications

Gene expression databases

GenevisibleiO04719. AT.

Interactioni

Subunit structurei

Interacts with SPK1, CIPK15/PKS3, GPX3, SCAR1, SCAR2, SCAR3 and SCARL. Interacts also with CIPK24/SOS2. Binds to the fibrillin precursor protein. Interacts with ABA-bounded PYR1, PYL1, PYL2, PYL3, PYL4, PYL5, PYL6, PYL8 and PYL9, and with free PYL2, PYL3 and PYL4.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CIPK24Q9LDI34EBI-537680,EBI-537551
PAP1O814395EBI-537680,EBI-962363
PYL9Q84MC73EBI-537680,EBI-2349513
PYR1O496863EBI-537680,EBI-2349590
SWI3BQ84JG22EBI-537680,EBI-1102271

Protein-protein interaction databases

BioGridi21053. 38 interactions.
DIPiDIP-35025N.
IntActiO04719. 8 interactions.
MINTiMINT-274841.
STRINGi3702.AT5G57050.1.

Structurei

Secondary structure

1
423
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi105 – 1084Combined sources
Beta strandi113 – 1186Combined sources
Beta strandi122 – 1243Combined sources
Beta strandi127 – 1326Combined sources
Helixi135 – 1395Combined sources
Helixi154 – 1563Combined sources
Beta strandi159 – 17012Combined sources
Helixi171 – 19121Combined sources
Helixi195 – 1973Combined sources
Helixi199 – 21719Combined sources
Helixi220 – 2245Combined sources
Beta strandi234 – 2396Combined sources
Beta strandi241 – 25111Combined sources
Beta strandi253 – 2586Combined sources
Beta strandi261 – 2666Combined sources
Helixi274 – 28310Combined sources
Beta strandi287 – 2959Combined sources
Turni296 – 2983Combined sources
Beta strandi299 – 3035Combined sources
Helixi308 – 3103Combined sources
Turni311 – 3133Combined sources
Beta strandi319 – 3246Combined sources
Beta strandi329 – 3357Combined sources
Helixi337 – 3404Combined sources
Helixi345 – 36117Combined sources
Helixi372 – 3765Combined sources
Beta strandi378 – 3803Combined sources
Helixi382 – 39716Combined sources
Beta strandi404 – 4107Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NMVX-ray2.10B101-423[»]
3UJKX-ray1.90A101-423[»]
3UJLX-ray2.50B101-423[»]
ProteinModelPortaliO04719.
SMRiO04719. Positions 103-415.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO04719.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini112 – 411300PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Domaini

The 'lock' site stabilizes the complex made of PP2C, ABA and PYR/PYL/RCAR receptor by keeping receptor 'gate' and 'latch' loops in closed positions.By similarity

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiO04719.
KOiK14497.
OMAiHKKNAMA.
OrthoDBiEOG09360DDO.
PhylomeDBiO04719.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 3 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O04719-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEVSPAVAV PFRPFTDPHA GLRGYCNGES RVTLPESSCS GDGAMKDSSF
60 70 80 90 100
EINTRQDSLT SSSSAMAGVD ISAGDEINGS DEFDPRSMNQ SEKKVLSRTE
110 120 130 140 150
SRSLFEFKCV PLYGVTSICG RRPEMEDSVS TIPRFLQVSS SSLLDGRVTN
160 170 180 190 200
GFNPHLSAHF FGVYDGHGGS QVANYCRERM HLALTEEIVK EKPEFCDGDT
210 220 230 240 250
WQEKWKKALF NSFMRVDSEI ETVAHAPETV GSTSVVAVVF PTHIFVANCG
260 270 280 290 300
DSRAVLCRGK TPLALSVDHK PDRDDEAARI EAAGGKVIRW NGARVFGVLA
310 320 330 340 350
MSRSIGDRYL KPSVIPDPEV TSVRRVKEDD CLILASDGLW DVMTNEEVCD
360 370 380 390 400
LARKRILLWH KKNAMAGEAL LPAEKRGEGK DPAAMSAAEY LSKMALQKGS
410 420
KDNISVVVVD LKGIRKFKSK SLN
Length:423
Mass (Da):46,306
Last modified:July 1, 1997 - v1
Checksum:i67CAAC76DA531A71
GO
Isoform 2 (identifier: O04719-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     29-68: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:383
Mass (Da):42,197
Checksum:i4E346383F40C3C34
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei29 – 6840Missing in isoform 2. CuratedVSP_034834Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08966 Genomic DNA. Translation: CAA70163.1.
Y08965 mRNA. Translation: CAA70162.1.
Y11840 Genomic DNA. Translation: CAA72538.1.
AB024035 Genomic DNA. Translation: BAA97035.1.
CP002688 Genomic DNA. Translation: AED96839.1.
CP002688 Genomic DNA. Translation: AED96840.1.
AY136415 mRNA. Translation: AAM97081.1.
BT008860 mRNA. Translation: AAP68299.1.
RefSeqiNP_001119448.1. NM_001125976.1. [O04719-2]
NP_200515.1. NM_125087.2. [O04719-1]
UniGeneiAt.22051.

Genome annotation databases

EnsemblPlantsiAT5G57050.1; AT5G57050.1; AT5G57050. [O04719-1]
GeneIDi835809.
KEGGiath:AT5G57050.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08966 Genomic DNA. Translation: CAA70163.1.
Y08965 mRNA. Translation: CAA70162.1.
Y11840 Genomic DNA. Translation: CAA72538.1.
AB024035 Genomic DNA. Translation: BAA97035.1.
CP002688 Genomic DNA. Translation: AED96839.1.
CP002688 Genomic DNA. Translation: AED96840.1.
AY136415 mRNA. Translation: AAM97081.1.
BT008860 mRNA. Translation: AAP68299.1.
RefSeqiNP_001119448.1. NM_001125976.1. [O04719-2]
NP_200515.1. NM_125087.2. [O04719-1]
UniGeneiAt.22051.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NMVX-ray2.10B101-423[»]
3UJKX-ray1.90A101-423[»]
3UJLX-ray2.50B101-423[»]
ProteinModelPortaliO04719.
SMRiO04719. Positions 103-415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21053. 38 interactions.
DIPiDIP-35025N.
IntActiO04719. 8 interactions.
MINTiMINT-274841.
STRINGi3702.AT5G57050.1.

PTM databases

iPTMnetiO04719.

Proteomic databases

PaxDbiO04719.
PRIDEiO04719.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G57050.1; AT5G57050.1; AT5G57050. [O04719-1]
GeneIDi835809.
KEGGiath:AT5G57050.

Organism-specific databases

TAIRiAT5G57050.

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiO04719.
KOiK14497.
OMAiHKKNAMA.
OrthoDBiEOG09360DDO.
PhylomeDBiO04719.

Enzyme and pathway databases

BioCyciARA:AT5G57050-MONOMER.
ARA:GQT-1437-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO04719.
PROiO04719.

Gene expression databases

GenevisibleiO04719. AT.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 3 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2C77_ARATH
AccessioniPrimary (citable) accession number: O04719
Secondary accession number(s): B3H561
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: September 7, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The reduced form of ABI2 is converted to the oxidized form by the addition of oxidized GPX3 or H2O2.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.