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Protein

Protein phosphatase 2C 77

Gene

ABI2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane (Pos), high light stress, response to glucose, seed germination and inhibition of vegetative growth. During the stomatal closure regulation, modulates the inward calcium-channel permeability as well as H2O2 and oxidative burst in response to ABA and dehydration. Controls negatively fibrillin that is involved in mediating ABA-induced photoprotection. May be implicated in ABA content regulation. Involved in acquired thermotolerance of root growth and seedling survival. Required for the Erwinia amylovora harpin-induced (HrpN) drought tolerance. Involved in the hydrotropic response.23 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+3 Publications, Mn2+1 PublicationNote: Binds 2 magnesium or manganese ions per subunit.3 Publications

Enzyme regulationi

Phosphatase activity repressed by oxidized ATGPX3, free fatty acids (e.g. arachidonic acid (20:4) and Linolenic acid (18:3)) and by H2O2. Repressed by PYR/PYL/RCAR ABA receptors in an ABA-dependent manner.3 Publications

pH dependencei

Optimum pH is 8.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi165Magnesium or manganese 1Combined sources1
Metal bindingi165Magnesium or manganese 2Combined sources1
Metal bindingi166Magnesium or manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi251Magnesium or manganese 1Combined sources1
Metal bindingi252Magnesium or manganese 1Combined sources1
Sitei290LockBy similarity1
Metal bindingi337Magnesium or manganese 1; via amide nitrogenCombined sources1
Metal bindingi337Magnesium or manganese 2Combined sources1
Metal bindingi341Magnesium or manganese 1By similarity1
Metal bindingi402Magnesium or manganese 2Combined sources1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • negative regulation of protein kinase activity Source: TAIR
  • photoinhibition Source: TAIR
  • regulation of stomatal opening Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to heat Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Abscisic acid signaling pathway

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 77 (EC:3.1.3.16)
Short name:
AtPP2C77
Alternative name(s):
Protein ABSCISIC ACID-INSENSITIVE 2
Protein phosphatase 2C ABI2
Short name:
PP2C ABI2
Gene namesi
Name:ABI2
Ordered Locus Names:At5g57050
ORF Names:MHM17.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G57050.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi168G → D in abi2; reduced phosphatase activity, reduced affinity with magnesium ions, loss of interaction with the fibrillin precursor protein, impaired ABA-mediated binding to PYR1, and reduced negative control on fibrillin activity. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000577671 – 423Protein phosphatase 2C 77Add BLAST423

Proteomic databases

PaxDbiO04719.

PTM databases

iPTMnetiO04719.

Expressioni

Inductioni

Repressed by MYB44 and ERF4. Induced by salt stress and ABA.4 Publications

Gene expression databases

GenevisibleiO04719. AT.

Interactioni

Subunit structurei

Interacts with SPK1, CIPK15/PKS3, GPX3, SCAR1, SCAR2, SCAR3 and SCARL. Interacts also with CIPK24/SOS2. Binds to the fibrillin precursor protein. Interacts with ABA-bounded PYR1, PYL1, PYL2, PYL3, PYL4, PYL5, PYL6, PYL8 and PYL9, and with free PYL2, PYL3 and PYL4.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CIPK24Q9LDI34EBI-537680,EBI-537551
PAP1O814395EBI-537680,EBI-962363
PYL9Q84MC73EBI-537680,EBI-2349513
PYR1O496863EBI-537680,EBI-2349590
SWI3BQ84JG22EBI-537680,EBI-1102271

Protein-protein interaction databases

BioGridi21053. 38 interactors.
DIPiDIP-35025N.
IntActiO04719. 8 interactors.
MINTiMINT-274841.
STRINGi3702.AT5G57050.1.

Structurei

Secondary structure

1423
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi105 – 108Combined sources4
Beta strandi113 – 118Combined sources6
Beta strandi122 – 124Combined sources3
Beta strandi127 – 132Combined sources6
Helixi135 – 139Combined sources5
Helixi154 – 156Combined sources3
Beta strandi159 – 170Combined sources12
Helixi171 – 191Combined sources21
Helixi195 – 197Combined sources3
Helixi199 – 217Combined sources19
Helixi220 – 224Combined sources5
Beta strandi234 – 239Combined sources6
Beta strandi241 – 251Combined sources11
Beta strandi253 – 258Combined sources6
Beta strandi261 – 266Combined sources6
Helixi274 – 283Combined sources10
Beta strandi287 – 295Combined sources9
Turni296 – 298Combined sources3
Beta strandi299 – 303Combined sources5
Helixi308 – 310Combined sources3
Turni311 – 313Combined sources3
Beta strandi319 – 324Combined sources6
Beta strandi329 – 335Combined sources7
Helixi337 – 340Combined sources4
Helixi345 – 361Combined sources17
Helixi372 – 376Combined sources5
Beta strandi378 – 380Combined sources3
Helixi382 – 397Combined sources16
Beta strandi404 – 410Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NMVX-ray2.10B101-423[»]
3UJKX-ray1.90A101-423[»]
3UJLX-ray2.50B101-423[»]
ProteinModelPortaliO04719.
SMRiO04719.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO04719.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini112 – 411PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST300

Domaini

The 'lock' site stabilizes the complex made of PP2C, ABA and PYR/PYL/RCAR receptor by keeping receptor 'gate' and 'latch' loops in closed positions.By similarity

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiO04719.
KOiK14497.
OMAiHKKNAMA.
OrthoDBiEOG09360DDO.
PhylomeDBiO04719.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 3 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O04719-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEVSPAVAV PFRPFTDPHA GLRGYCNGES RVTLPESSCS GDGAMKDSSF
60 70 80 90 100
EINTRQDSLT SSSSAMAGVD ISAGDEINGS DEFDPRSMNQ SEKKVLSRTE
110 120 130 140 150
SRSLFEFKCV PLYGVTSICG RRPEMEDSVS TIPRFLQVSS SSLLDGRVTN
160 170 180 190 200
GFNPHLSAHF FGVYDGHGGS QVANYCRERM HLALTEEIVK EKPEFCDGDT
210 220 230 240 250
WQEKWKKALF NSFMRVDSEI ETVAHAPETV GSTSVVAVVF PTHIFVANCG
260 270 280 290 300
DSRAVLCRGK TPLALSVDHK PDRDDEAARI EAAGGKVIRW NGARVFGVLA
310 320 330 340 350
MSRSIGDRYL KPSVIPDPEV TSVRRVKEDD CLILASDGLW DVMTNEEVCD
360 370 380 390 400
LARKRILLWH KKNAMAGEAL LPAEKRGEGK DPAAMSAAEY LSKMALQKGS
410 420
KDNISVVVVD LKGIRKFKSK SLN
Length:423
Mass (Da):46,306
Last modified:July 1, 1997 - v1
Checksum:i67CAAC76DA531A71
GO
Isoform 2 (identifier: O04719-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     29-68: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:383
Mass (Da):42,197
Checksum:i4E346383F40C3C34
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03483429 – 68Missing in isoform 2. CuratedAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08966 Genomic DNA. Translation: CAA70163.1.
Y08965 mRNA. Translation: CAA70162.1.
Y11840 Genomic DNA. Translation: CAA72538.1.
AB024035 Genomic DNA. Translation: BAA97035.1.
CP002688 Genomic DNA. Translation: AED96839.1.
CP002688 Genomic DNA. Translation: AED96840.1.
AY136415 mRNA. Translation: AAM97081.1.
BT008860 mRNA. Translation: AAP68299.1.
RefSeqiNP_001119448.1. NM_001125976.2. [O04719-2]
NP_200515.1. NM_125087.3. [O04719-1]
UniGeneiAt.22051.

Genome annotation databases

EnsemblPlantsiAT5G57050.1; AT5G57050.1; AT5G57050. [O04719-1]
GeneIDi835809.
GrameneiAT5G57050.1; AT5G57050.1; AT5G57050.
KEGGiath:AT5G57050.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08966 Genomic DNA. Translation: CAA70163.1.
Y08965 mRNA. Translation: CAA70162.1.
Y11840 Genomic DNA. Translation: CAA72538.1.
AB024035 Genomic DNA. Translation: BAA97035.1.
CP002688 Genomic DNA. Translation: AED96839.1.
CP002688 Genomic DNA. Translation: AED96840.1.
AY136415 mRNA. Translation: AAM97081.1.
BT008860 mRNA. Translation: AAP68299.1.
RefSeqiNP_001119448.1. NM_001125976.2. [O04719-2]
NP_200515.1. NM_125087.3. [O04719-1]
UniGeneiAt.22051.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NMVX-ray2.10B101-423[»]
3UJKX-ray1.90A101-423[»]
3UJLX-ray2.50B101-423[»]
ProteinModelPortaliO04719.
SMRiO04719.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21053. 38 interactors.
DIPiDIP-35025N.
IntActiO04719. 8 interactors.
MINTiMINT-274841.
STRINGi3702.AT5G57050.1.

PTM databases

iPTMnetiO04719.

Proteomic databases

PaxDbiO04719.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G57050.1; AT5G57050.1; AT5G57050. [O04719-1]
GeneIDi835809.
GrameneiAT5G57050.1; AT5G57050.1; AT5G57050.
KEGGiath:AT5G57050.

Organism-specific databases

TAIRiAT5G57050.

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiO04719.
KOiK14497.
OMAiHKKNAMA.
OrthoDBiEOG09360DDO.
PhylomeDBiO04719.

Miscellaneous databases

EvolutionaryTraceiO04719.
PROiO04719.

Gene expression databases

GenevisibleiO04719. AT.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 3 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2C77_ARATH
AccessioniPrimary (citable) accession number: O04719
Secondary accession number(s): B3H561
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The reduced form of ABI2 is converted to the oxidized form by the addition of oxidized GPX3 or H2O2.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.