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Protein

Threonine--tRNA ligase, mitochondrial 1

Gene

THRRS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi407Zinc; catalyticBy similarity1
Metal bindingi458Zinc; catalyticBy similarity1
Metal bindingi584Zinc; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligase, mitochondrial 1Curated (EC:6.1.1.3Curated)
Alternative name(s):
AtSYT11 Publication
Threonyl-tRNA synthetaseCurated
Short name:
ThrRSCurated
Gene namesi
Name:THRRS1 Publication
Ordered Locus Names:At5g26830
ORF Names:F2P16.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G26830
TAIRilocus:2148538 AT5G26830

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 21MitochondrionSequence analysisAdd BLAST21
ChainiPRO_000003582422 – 709Threonine--tRNA ligase, mitochondrial 1Add BLAST688

Proteomic databases

PaxDbiO04630
PRIDEiO04630

Expressioni

Gene expression databases

ExpressionAtlasiO04630 baseline and differential
GenevisibleiO04630 AT

Interactioni

Protein-protein interaction databases

BioGridi180164 interactors.
IntActiO04630 1 interactor.
STRINGi3702.AT5G26830.1

Structurei

3D structure databases

ProteinModelPortaliO04630
SMRiO04630
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1637 Eukaryota
COG0441 LUCA
HOGENOMiHOG000003878
InParanoidiO04630
KOiK01868
OMAiYWEGRSD
OrthoDBiEOG093603QZ
PhylomeDBiO04630

Family and domain databases

CDDicd00771 ThrRS_core, 1 hit
Gene3Di3.10.20.301 hit
3.40.50.8001 hit
HAMAPiMF_00184 Thr_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR012675 Beta-grasp_dom_sf
IPR004095 TGS
IPR012676 TGS-like
IPR002320 Thr-tRNA-ligase_IIa
IPR018163 Thr/Ala-tRNA-synth_IIc_edit
IPR033728 ThrRS_core
IPR012947 tRNA_SAD
PANTHERiPTHR11451 PTHR11451, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF02824 TGS, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF07973 tRNA_SAD, 1 hit
PRINTSiPR01047 TRNASYNTHTHR
SMARTiView protein in SMART
SM00863 tRNA_SAD, 1 hit
SUPFAMiSSF55186 SSF55186, 1 hit
SSF81271 SSF81271, 1 hit
TIGRFAMsiTIGR00418 thrS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: O04630-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLRLTARSI RRFTTSSSSL PLLSSSSFCT VPTMAANHPK DEAYLSAVIP
60 70 80 90 100
KRIKLFEQIQ ANQLENLKSL PHDPIKVTLP DGNVKEGKKW ETTPMDIAAQ
110 120 130 140 150
ISKGLANSAL ISAVDDVLWD MNRPLEGDCK LELFKFDSDK GRDTLWHSSA
160 170 180 190 200
HILGQALEQE YGCQLCIGPC TTRGEGFYYD GFYGELGLSD NHFPSIEAGA
210 220 230 240 250
AKAAKEAQPF ERIEVTKDQA LEMFSENNFK VELINGLPAD MTITVYRCGP
260 270 280 290 300
LVDLCRGPHI PNTSFVKAFK CLRASSAYWK GDKDRESLQR VYGISYPDQK
310 320 330 340 350
QLKKYLQFLE EAKKYDHRLL GQKQELFFSH QLSPGSYFFL PLGTRVYNRL
360 370 380 390 400
MDFIKNQYWH RGYTEVITPN MYNMELWQTS GHADNYKDNM FTFNIEKQEF
410 420 430 440 450
GLKPMNCPGH CLIFQHRVRS YRELPMRLAD FGVLHRNEAS GALSGLTRVR
460 470 480 490 500
RFQQDDAHIF CTTEQVKGEV QGVLEFIDYV YKVFGFTYEL KLSTRPEKYL
510 520 530 540 550
GDLETWDKAE ADLKEAIEAF GKPLVLNEGD GAFYGPKIDI TVSDAMNRKF
560 570 580 590 600
QCATLQLDFQ LPIRFNLEYA AEDEAKKSRP VMIHRAVLGS VERMFAILLE
610 620 630 640 650
HYKGKWPFWI SPRQAIVCPI SEKSQQYAEK VQKQIKDAGF YVDADLTDRK
660 670 680 690 700
IDKKVREAQL AQYNYILVVG ETEAATGQVS VRVRDNAAHS VKSIEDLLEE

FKAKTAEFV
Length:709
Mass (Da):80,936
Last modified:January 10, 2003 - v3
Checksum:iA74FFFD7A6B0B5CF
GO
Isoform Cytoplasmic (identifier: O04630-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.

Show »
Length:676
Mass (Da):77,337
Checksum:iB57A2A7581BFA4C5
GO

Sequence cautioni

The sequence AAB61048 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121M → V in AAK82468 (PubMed:14593172).Curated1
Sequence conflicti121M → V in AAO64760 (PubMed:14593172).Curated1
Sequence conflicti426M → L in CAA74705 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189081 – 33Missing in isoform Cytoplasmic. CuratedAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14329 mRNA Translation: CAA74705.1
AF007270 Genomic DNA Translation: AAB61048.1 Sequence problems.
CP002688 Genomic DNA Translation: AED93610.1
AY048205 mRNA Translation: AAK82468.1
BT005825 mRNA Translation: AAO64760.1
PIRiT01763
T51624
RefSeqiNP_198035.2, NM_122565.3 [O04630-1]
UniGeneiAt.23575
At.55026

Genome annotation databases

EnsemblPlantsiAT5G26830.1; AT5G26830.1; AT5G26830 [O04630-1]
GeneIDi832741
GrameneiAT5G26830.1; AT5G26830.1; AT5G26830 [O04630-1]
KEGGiath:AT5G26830

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiSYTM1_ARATH
AccessioniPrimary (citable) accession number: O04630
Secondary accession number(s): O81907, Q84TG4, Q94AG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 10, 2003
Last modified: April 25, 2018
This is version 151 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome