Reviewed, UniProtKB/Swiss-Prot O04567 (Y1719_ARATH)

Names and origin

Protein names

Recommended name:
    Probable inactive receptor kinase At1g27190

Gene names

Ordered Locus Names:	At1g27190
ORF Names:	T7N9.25

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Membrane; Single-pass membrane protein Potential.
Domain	The protein kinase domain is predicted to be catalytically inactive.
Post-translational modification	Phosphorylation level varies significantly during early response to general elicitors.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 5 LRR (leucine-rich) repeats. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Leucine-rich repeat Repeat Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Ref.5 Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CALM	P62157	1	EBI-1238687,EBI-397403	From a different organism.
CAM1	P25854	1	EBI-1238687,EBI-1235664
CAM6	Q03509	1	EBI-1238687,EBI-1236097
CAM7	P59220	1	EBI-1238687,EBI-1236031
CML10	P30187	1	EBI-1238687,EBI-1238722
CML9	Q9S744	1	EBI-1238687,EBI-1236048

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O04567-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O04567-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-294: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 601	577	Probable inactive receptor kinase At1g27190		PRO_0000305184
Regions
	Transmembrane	221 – 241	21	Potential
	Repeat	71 – 95	25	LRR 1
	Repeat	96 – 119	24	LRR 2
	Repeat	121 – 144	24	LRR 3
	Repeat	145 – 168	24	LRR 4
	Repeat	169 – 192	24	LRR 5
	Domain	301 – 586	286	Protein kinase
	Nucleotide binding	307 – 315	9	ATP By similarity
Sites
	Binding site	329	1	ATP By similarity
Amino acid modifications
	Modified residue	586	1	Phosphoserine Ref.4 Ref.5
	Glycosylation	52	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 294	294	Missing in isoform 2.		VSP_028261

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 7A263A3076F5D8A9	FASTA	601	65,424
	10 20 30 40 50 60 MKKIFITLLW LLFISSFLCS SSSAEDDVLC LQGLKNSLID PSSRLSSWSF PNSSASSICK 70 80 90 100 110 120 LTGVSCWNEK ENRIISLQLQ SMQLAGEIPE SLKLCRSLQS LDLSGNDLSG SIPSQICSWL 130 140 150 160 170 180 PYLVTLDLSG NKLGGSIPTQ IVECKFLNAL ILSDNKLSGS IPSQLSRLDR LRRLSLAGND 190 200 210 220 230 240 LSGTIPSELA RFGGDDFSGN NGLCGKPLSR CGALNGRNLS IIIVAGVLGA VGSLCVGLVI 250 260 270 280 290 300 FWWFFIREGS RKKKGYGAGK SKDDSDWIGL LRSHKLVQVT LFQKPIVKIK LGDLMAATNN 310 320 330 340 350 360 FSSGNIDVSS RTGVSYKADL PDGSALAVKR LSACGFGEKQ FRSEMNKLGE LRHPNLVPLL 370 380 390 400 410 420 GYCVVEDERL LVYKHMVNGT LFSQLHNGGL CDAVLDWPTR RAIGVGAAKG LAWLHHGCQP 430 440 450 460 470 480 PYLHQFISSN VILLDDDFDA RITDYGLAKL VGSRDSNDSS FNNGDLGELG YVAPEYSSTM 490 500 510 520 530 540 VASLKGDVYG FGIVLLELVT GQKPLSVING VEGFKGSLVD WVSQYLGTGR SKDAIDRSIC 550 560 570 580 590 600 DKGHDEEILQ FLKIACSCVV SRPKERPTMI QVYESLKNMA DKHGVSEHYD EFPLVFNKQE A
	Isoform 2. Checksum: 0D19198433301DB9	FASTA	307	33,613
	10 20 30 40 50 60 MAATNNFSSG NIDVSSRTGV SYKADLPDGS ALAVKRLSAC GFGEKQFRSE MNKLGELRHP 70 80 90 100 110 120 NLVPLLGYCV VEDERLLVYK HMVNGTLFSQ LHNGGLCDAV LDWPTRRAIG VGAAKGLAWL 130 140 150 160 170 180 HHGCQPPYLH QFISSNVILL DDDFDARITD YGLAKLVGSR DSNDSSFNNG DLGELGYVAP 190 200 210 220 230 240 EYSSTMVASL KGDVYGFGIV LLELVTGQKP LSVINGVEGF KGSLVDWVSQ YLGTGRSKDA 250 260 270 280 290 300 IDRSICDKGH DEEILQFLKI ACSCVVSRPK ERPTMIQVYE SLKNMADKHG VSEHYDEFPL VFNKQEA

References

[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[4]	"Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis." Benschop J.J., Mohammed S., O'Flaherty M., Heck A.J.R., Slijper M., Menke F.L.H. Mol. Cell. Proteomics 6:1198-1214(2007) [PubMed: 17317660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-586, MASS SPECTROMETRY.
[5]	"Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis." de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E., Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J., Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H. J. Proteome Res. 7:2458-2470(2008) [PubMed: 18433157] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-586, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC000348 Genomic DNA. Translation: AAF79872.1. BT015801 mRNA. Translation: AAU94364.1. AK228940 mRNA. Translation: BAF00829.1.
IPI	IPI00523297. IPI00867960.
RefSeq	NP_174039.1.
UniGene	At.16279
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O04567. 6 interactions.
Proteomic databases
PRIDE	O04567.
Genome annotation databases
GeneID	839608.
GenomeReviews	Gene locus AT1G27190 in contig CT485782_GR.
KEGG	ath:AT1G27190.
NMPDR	fig|3702.1.peg.3042.
Organism-specific databases
GeneFarm	2512. 54.
TAIR	At1g27190.
Phylogenomic databases
OMA	O04567. DARITDY.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. IPR013210. Leu-rich_rpt_N. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR001245. Tyr_pkinase. [Graphical view]
Pfam	PF00560. LRR_1. 4 hits. PF08263. LRRNT_2. 1 hit. PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y1719_ARATH

Accession

Primary (citable) accession number: O04567
Secondary accession number(s): Q0WPX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	July 1, 1997
Last modified:	July 7, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)