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Protein

Probable elongation factor 1-gamma 1

Gene

At1g09640

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Probably plays a role in anchoring the complex to other cellular components.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-ATH-156842. Eukaryotic Translation Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable elongation factor 1-gamma 1
Short name:
EF-1-gamma 1
Alternative name(s):
eEF-1B gamma 1
Gene namesi
Ordered Locus Names:At1g09640
ORF Names:F21M12.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G09640.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: TAIR
  • nucleus Source: GO_Central
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414Probable elongation factor 1-gamma 1PRO_0000208826Add
BLAST

Proteomic databases

PaxDbiO04487.
PRIDEiO04487.

PTM databases

iPTMnetiO04487.
SwissPalmiO04487.

Expressioni

Gene expression databases

ExpressionAtlasiO04487. baseline and differential.
GenevisibleiO04487. AT.

Interactioni

Subunit structurei

EF-1 is composed of four subunits: alpha, beta, delta, and gamma.By similarity

Protein-protein interaction databases

BioGridi22732. 1 interaction.
STRINGi3702.AT1G09640.1.

Structurei

3D structure databases

ProteinModelPortaliO04487.
SMRiO04487. Positions 17-199, 254-414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8282GST N-terminalAdd
BLAST
Domaini87 – 215129GST C-terminalAdd
BLAST
Domaini254 – 414161EF-1-gamma C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 EF-1-gamma C-terminal domain.PROSITE-ProRule annotation
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
KOG1627. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000201196.
InParanoidiO04487.
KOiK03233.
OMAiDGWSLWF.
PhylomeDBiO04487.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.30.70.1010. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
IPR001662. Transl_elong_EF1_G_con.
[Graphical view]
PfamiPF00647. EF1G. 1 hit.
PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SMARTiSM01183. EF1G. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
SSF89942. SSF89942. 1 hit.
PROSITEiPS50040. EF1G_C. 1 hit.
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O04487-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALVLHTYKG NKSAEKALIA AEYVGVQIDV PSDFQMGVTN KTPAFLKMNP
60 70 80 90 100
IGKVPVLETP EGSVFESNAI ARYVSRLNGD NSLNGSSLIE YAQIEQWIDF
110 120 130 140 150
SSLEIYASIL RWFGPRMGFM PYSAPAEEGA ISTLKRALDA LNTHLTSNTY
160 170 180 190 200
LVGHSITLAD IITVCNLNLG FATVMTKKFT SEFPHVERYF WTVVNQPNFT
210 220 230 240 250
KVLGDVKQTE AVPPIASKKA AQPAKPKEEP KKKEAPVAEA PKLAEEEEAP
260 270 280 290 300
KPKAKNPLDL LPPSPMVLDD WKRLYSNTKS NFREVAIKGF WDMYDPEGYS
310 320 330 340 350
LWFCDYKYND ENMVSFVTLN KVGGFLQRMD LARKYSFGKM LICGSEGPFK
360 370 380 390 400
VKGLWLFRGP EIPKFIMDEV YDMELYEWTK VDISDEAQKE RVSQMIEDAE
410
PFEGEALLDA KCFK
Length:414
Mass (Da):46,661
Last modified:July 1, 1997 - v1
Checksum:iD41EC8C9C0B03C5D
GO

Sequence cautioni

The sequence AY089154 differs from that shown. Reason: Frameshift at position 25. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC000132 Genomic DNA. Translation: AAB60721.1.
CP002684 Genomic DNA. Translation: AEE28472.1.
AY089154 mRNA. No translation available.
AY080820 mRNA. Translation: AAL87298.1.
PIRiB86230.
RefSeqiNP_563848.1. NM_100836.3. [O04487-1]
UniGeneiAt.23720.
At.43279.

Genome annotation databases

EnsemblPlantsiAT1G09640.1; AT1G09640.1; AT1G09640. [O04487-1]
GeneIDi837491.
KEGGiath:AT1G09640.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC000132 Genomic DNA. Translation: AAB60721.1.
CP002684 Genomic DNA. Translation: AEE28472.1.
AY089154 mRNA. No translation available.
AY080820 mRNA. Translation: AAL87298.1.
PIRiB86230.
RefSeqiNP_563848.1. NM_100836.3. [O04487-1]
UniGeneiAt.23720.
At.43279.

3D structure databases

ProteinModelPortaliO04487.
SMRiO04487. Positions 17-199, 254-414.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22732. 1 interaction.
STRINGi3702.AT1G09640.1.

PTM databases

iPTMnetiO04487.
SwissPalmiO04487.

Proteomic databases

PaxDbiO04487.
PRIDEiO04487.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G09640.1; AT1G09640.1; AT1G09640. [O04487-1]
GeneIDi837491.
KEGGiath:AT1G09640.

Organism-specific databases

TAIRiAT1G09640.

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
KOG1627. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000201196.
InParanoidiO04487.
KOiK03233.
OMAiDGWSLWF.
PhylomeDBiO04487.

Enzyme and pathway databases

ReactomeiR-ATH-156842. Eukaryotic Translation Elongation.

Miscellaneous databases

PROiO04487.

Gene expression databases

ExpressionAtlasiO04487. baseline and differential.
GenevisibleiO04487. AT.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.30.70.1010. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
IPR001662. Transl_elong_EF1_G_con.
[Graphical view]
PfamiPF00647. EF1G. 1 hit.
PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SMARTiSM01183. EF1G. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
SSF89942. SSF89942. 1 hit.
PROSITEiPS50040. EF1G_C. 1 hit.
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 70-414.
    Strain: cv. Columbia.

Entry informationi

Entry nameiEF1G1_ARATH
AccessioniPrimary (citable) accession number: O04487
Secondary accession number(s): Q8RXL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 1, 1997
Last modified: July 6, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.