O04478 (GUN7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 7
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 7

Gene names

Name:	KOR2
Ordered Locus Names:	At1g65610
ORF Names:	F5I14.14

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Expressed in basal region of leaf blade and proximal parts of leaf and floral organ. Ref.4
Developmental stage	Early expressed in the development of root hairs whithin the root differentiation zone. Expressed late in the development of leaf trichomes when the stalks and branches are expanded. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	Endoglucanase 7		PRO_0000249260
Regions
	Topological domain	1 – 79	79	Cytoplasmic Potential
	Transmembrane	80 – 100	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	101 – 623	523	Extracellular Potential
Sites
	Active site	517	1	By similarity
	Active site	565	1	By similarity
	Active site	574	1	By similarity
Amino acid modifications
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	221	1	N-linked (GlcNAc...) Potential
	Glycosylation	328	1	N-linked (GlcNAc...) Potential
	Glycosylation	349	1	N-linked (GlcNAc...) Potential
	Glycosylation	412	1	N-linked (GlcNAc...) Potential
	Glycosylation	429	1	N-linked (GlcNAc...) Potential
	Glycosylation	464	1	N-linked (GlcNAc...) Potential
	Glycosylation	548	1	N-linked (GlcNAc...) Potential
	Glycosylation	571	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O04478 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 8186D6B5497D1FD4	FASTA	623	69,836
	10 20 30 40 50 60 MHPGNVWGGS LDAVDSDRIA AEEEERLRNT TEWDRGAIHS QRSELDETQQ GWLLAPQDNW 70 80 90 100 110 120 RKKKKKYVNL GCVSVSRTVF LWTVGSIAVL FLVVALPIII VKSLPRHKSA PPPPDNYTLA 130 140 150 160 170 180 LHKALQFFDA QKSGKLPKKN KVSWRGDSGT KDGLPDVVGG LVGGYYDGGS NVKFHFPMAF 190 200 210 220 230 240 SMTMLSWSLI EYSHKYKAID EYDHMRDVLK WGTDYLLLTF NNSATRLDHI YTQVGGGLRD 250 260 270 280 290 300 SESPDDIYCW QKPEDMSYDR PVLSSTSAAD LGAEVSAALA AASIVFTDKP DYAKKLKKGA 310 320 330 340 350 360 ETLYPFFRSK SRRKRYSDGQ PTAQAFYNST SMFDEFMWAG AWLYYATGNK TYIQFATTPS 370 380 390 400 410 420 VPQTAKAFAN RPELMVPSWN NKLPGAMLLM TRYRLFLNPG FPYENMLNRY HNATGITMCA 430 440 450 460 470 480 YLKQYNVFNR TSGGLMQLNL GKPRPLEYVA HASFLASLFA DYLNSTGVPG WYCGPTFVEN 490 500 510 520 530 540 HVLKDFAQSQ IDYILGDNPL KMSYVVGFGK KFPRRVHHRG ATIPNDKKRR SCREGLKYRD 550 560 570 580 590 600 TKNPNPNNIT GAMVGGPNKF DEFHDLRNNY NASEPTLSGN AGLVAALVSL TSSGGQQIDK 610 620 NTMFNSVPPL YSPTPPPPKA WKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Two Arabidopsis thaliana genes, KOR2 and KOR3, which encode membrane-anchored endo-1,4-beta-D-glucanases, are differentially expressed in developing leaf trichomes and their support cells." Moelhoej M., Joergensen B., Ulvskov P., Borkhardt B. Plant Mol. Biol. 46:263-275(2001) [PubMed: 11488474] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed: 15170254] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC001229 Genomic DNA. Translation: AAB60922.1. CP002684 Genomic DNA. Translation: AEE34400.1. AK229402 mRNA. Translation: BAF01264.1.
IPI	IPI00531295.
PIR	B96681.
RefSeq	NP_176738.1. NM_105234.2.
UniGene	At.35864.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	O04478.
SMR	O04478. Positions 112-601.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PRIDE	O04478.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G65610.1; AT1G65610.1; AT1G65610.
GeneID	842872.
GenomeReviews	Gene locus AT1G65610 in contig CT485782_GR.
KEGG	ath:AT1G65610.
NMPDR	fig|3702.1.peg.5984.
Organism-specific databases
TAIR	At1g65610.
Phylogenomic databases
GeneTree	EPGT00070000028567.
HOGENOM	HBG746347.
InParanoid	O04478.
OMA	EYVAHAS.
PhylomeDB	O04478.
ProtClustDB	CLSN2679649.
Gene expression databases
Genevestigator	O04478.
GermOnline	AT1G65610. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN7_ARATH

Accession

Primary (citable) accession number: O04478

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	July 1, 1997
Last modified:	December 14, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program