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O04478 (GUN7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 7

EC=3.2.1.4
Alternative name(s):
Endo-1,4-beta glucanase 7
Gene names
Name:KOR2
Ordered Locus Names:At1g65610
ORF Names:F5I14.14
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length623 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Membrane; Single-pass type II membrane protein Potential.

Tissue specificity

Expressed in basal region of leaf blade and proximal parts of leaf and floral organ. Ref.4

Developmental stage

Early expressed in the development of root hairs within the root differentiation zone. Expressed late in the development of leaf trichomes when the stalks and branches are expanded. Ref.4

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 623623Endoglucanase 7
PRO_0000249260

Regions

Topological domain1 – 7979Cytoplasmic Potential
Transmembrane80 – 10021Helical; Signal-anchor for type II membrane protein; Potential
Topological domain101 – 623523Extracellular Potential

Sites

Active site5171 By similarity
Active site5651 By similarity
Active site5741 By similarity

Amino acid modifications

Glycosylation1161N-linked (GlcNAc...) Potential
Glycosylation2211N-linked (GlcNAc...) Potential
Glycosylation3281N-linked (GlcNAc...) Potential
Glycosylation3491N-linked (GlcNAc...) Potential
Glycosylation4121N-linked (GlcNAc...) Potential
Glycosylation4291N-linked (GlcNAc...) Potential
Glycosylation4641N-linked (GlcNAc...) Potential
Glycosylation5481N-linked (GlcNAc...) Potential
Glycosylation5711N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O04478 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 8186D6B5497D1FD4

FASTA62369,836
        10         20         30         40         50         60 
MHPGNVWGGS LDAVDSDRIA AEEEERLRNT TEWDRGAIHS QRSELDETQQ GWLLAPQDNW 

        70         80         90        100        110        120 
RKKKKKYVNL GCVSVSRTVF LWTVGSIAVL FLVVALPIII VKSLPRHKSA PPPPDNYTLA 

       130        140        150        160        170        180 
LHKALQFFDA QKSGKLPKKN KVSWRGDSGT KDGLPDVVGG LVGGYYDGGS NVKFHFPMAF 

       190        200        210        220        230        240 
SMTMLSWSLI EYSHKYKAID EYDHMRDVLK WGTDYLLLTF NNSATRLDHI YTQVGGGLRD 

       250        260        270        280        290        300 
SESPDDIYCW QKPEDMSYDR PVLSSTSAAD LGAEVSAALA AASIVFTDKP DYAKKLKKGA 

       310        320        330        340        350        360 
ETLYPFFRSK SRRKRYSDGQ PTAQAFYNST SMFDEFMWAG AWLYYATGNK TYIQFATTPS 

       370        380        390        400        410        420 
VPQTAKAFAN RPELMVPSWN NKLPGAMLLM TRYRLFLNPG FPYENMLNRY HNATGITMCA 

       430        440        450        460        470        480 
YLKQYNVFNR TSGGLMQLNL GKPRPLEYVA HASFLASLFA DYLNSTGVPG WYCGPTFVEN 

       490        500        510        520        530        540 
HVLKDFAQSQ IDYILGDNPL KMSYVVGFGK KFPRRVHHRG ATIPNDKKRR SCREGLKYRD 

       550        560        570        580        590        600 
TKNPNPNNIT GAMVGGPNKF DEFHDLRNNY NASEPTLSGN AGLVAALVSL TSSGGQQIDK 

       610        620 
NTMFNSVPPL YSPTPPPPKA WKP 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Two Arabidopsis thaliana genes, KOR2 and KOR3, which encode membrane-anchored endo-1,4-beta-D-glucanases, are differentially expressed in developing leaf trichomes and their support cells."
Moelhoej M., Joergensen B., Ulvskov P., Borkhardt B.
Plant Mol. Biol. 46:263-275(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
Libertini E., Li Y., McQueen-Mason S.J.
J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC001229 Genomic DNA. Translation: AAB60922.1.
CP002684 Genomic DNA. Translation: AEE34400.1.
AK229402 mRNA. Translation: BAF01264.1.
PIRB96681.
RefSeqNP_176738.1. NM_105234.2.
UniGeneAt.35864.

3D structure databases

ProteinModelPortalO04478.
SMRO04478. Positions 113-616.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G65610.1-P.

Protein family/group databases

CAZyGH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbO04478.
PRIDEO04478.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G65610.1; AT1G65610.1; AT1G65610.
GeneID842872.
KEGGath:AT1G65610.

Organism-specific databases

TAIRAT1G65610.

Phylogenomic databases

eggNOGNOG307789.
HOGENOMHOG000021033.
InParanoidO04478.
OMAEYVAHAS.
PhylomeDBO04478.
ProtClustDBCLSN2679649.

Enzyme and pathway databases

BioCycARA:AT1G65610-MONOMER.

Gene expression databases

GenevestigatorO04478.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN7_ARATH
AccessionPrimary (citable) accession number: O04478
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: July 1, 1997
Last modified: February 19, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names