Reviewed,
UniProtKB/Swiss-Prot O04478 (GUN7_ARATH)
Last modified
November 3, 2009.
Version 60.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Endoglucanase 7 EC=3.2.1.4 Alternative name(s): Endo-1,4-beta glucanase 7 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 623 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. |
| Subcellular location | Membrane; Single-pass type II membrane protein Potential. |
| Tissue specificity | Expressed in basal region of leaf blade and proximal parts of leaf and floral organ. Ref.3 |
| Developmental stage | Early expressed in the development of root hairs whithin the root differentiation zone. Expressed late in the development of leaf trichomes when the stalks and branches are expanded. Ref.3 |
| Sequence similarities | Belongs to the glycosyl hydrolase 9 (cellulase E) family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation |
| Cellular component | Membrane |
| Domain | Signal-anchor Transmembrane |
| Molecular function | Glycosidase Hydrolase |
| PTM | Glycoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | cellulase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 623 | 623 | Endoglucanase 7 | PRO_0000249260 | |||||
Regions | |||||||||
| Topological domain | 1 – 79 | 79 | Cytoplasmic Potential | ||||||
| Transmembrane | 80 – 100 | 21 | Signal-anchor for type II membrane protein Potential | ||||||
| Topological domain | 101 – 623 | 523 | Extracellular Potential | ||||||
Sites | |||||||||
| Active site | 517 | 1 | By similarity | ||||||
| Active site | 565 | 1 | By similarity | ||||||
| Active site | 574 | 1 | By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 116 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 221 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 328 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 349 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 412 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 429 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 464 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 548 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 571 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K.Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [3] | "Two Arabidopsis thaliana genes, KOR2 and KOR3, which encode membrane-anchored endo-1,4-beta-D-glucanases, are differentially expressed in developing leaf trichomes and their support cells." Moelhoej M., Joergensen B., Ulvskov P., Borkhardt B. Plant Mol. Biol. 46:263-275(2001) [PubMed: 11488474] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. |
| [4] | "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed: 15170254] [Abstract] Cited for: GENE FAMILY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AC001229 Genomic DNA. Translation: AAB60922.1. AK229402 mRNA. Translation: BAF01264.1. | |
| IPI | IPI00531295. |
| PIR | B96681. |
| RefSeq | NP_176738.1. |
| UniGene | At.35864 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1IA6 based on UniProtKB Q9EYQ2. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH9. Glycoside Hydrolase Family 9. |
Proteomic databases | |
| PRIDE | O04478. |
Genome annotation databases | |
| GeneID | 842872. |
| GenomeReviews | Gene locus AT1G65610 in contig CT485782_GR. |
| KEGG | ath:AT1G65610. |
| NMPDR | fig|3702.1.peg.5984. |
Organism-specific databases | |
| TAIR | At1g65610. |
Phylogenomic databases | |
| OMA | NNITGAM. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.4. 302. |
Gene expression databases | |
| Genevestigator | O04478. |
| GermOnline | AT1G65610. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view] |
| Gene3D | G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit. |
| PANTHER | PTHR22298:SF3. Glyco_hydro_9. 1 hit. |
| Pfam | PF00759. Glyco_hydro_9. 1 hit. [Graphical view] |
| PROSITE | PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GUN7_ARATH | ||||||||
| Accession | Primary (citable) accession number: O04478 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


