Reviewed, UniProtKB/Swiss-Prot O04425 (FCA_ARATH)

Names and origin

Protein names

Recommended name:
    Flowering time control protein FCA

Gene names

Name:	FCA
Ordered Locus Names:	At4g16280, At4g16290, At4g16300
ORF Names:	dl4180c, dl4181c, dl4182c, FCAALL.331

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	747 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a major role in the promotion of the transition of the vegetative meristem to reproductive development.
Sequence similarities	Contains 2 RRM (RNA recognition motif) domains. Contains 1 WW domain.

Ontologies

Keywords
Biological process	Differentiation Flowering
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW chromatin silencing by small RNA Inferred from mutant phenotype. Source: TAIR embryo sac development Inferred from genetic interaction. Source: TAIR embryonic development Inferred from genetic interaction. Source: TAIR flower development Inferred from electronic annotation. Source: UniProtKB-KW regulation of flower development Inferred from mutant phenotype. Source: TAIR
Cellular component	chromatin Traceable author statement. Source: TAIR
Molecular function	RNA binding Ref.1 Inferred from direct assay. Source: TAIR nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform Gamma (identifier: O04425-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Alpha (identifier: O04425-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-242: Missing.

Isoform Beta (identifier: O04425-3)

The sequence of this isoform differs from the canonical sequence as follows:
     163-270: CCFVKYATSK...KETAMAAIDG → MSISILLGNS...LDVFEKCRIY
     271-747: Missing.

Isoform Delta (identifier: O04425-4)

The sequence of this isoform differs from the canonical sequence as follows:
     534-747: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 747	747	Flowering time control protein FCA		PRO_0000081588
Regions
	Domain	120 – 201	82	RRM 1
	Domain	211 – 291	81	RRM 2
	Domain	591 – 624	34	WW
	Compositional bias	493 – 558	66	Gln-rich
	Compositional bias	632 – 672	41	Gln-rich
Natural variations
	Alternative sequence	1 – 242	242	Missing in isoform Alpha.		VSP_005794
	Alternative sequence	163 – 270	108	CCFVK…AAIDG → MSISILLGNSRELYFLKFQV SLKRLMGSNPSLIIGLQLFA TIFGLIAYACQRLICVRFCH RRRLPVLRKGLCTYVGQIDF ATCVYSRTLDVFEKCRIY in isoform Beta.		VSP_005795
	Alternative sequence	271 – 747	477	Missing in isoform Beta.		VSP_005796
	Alternative sequence	534 – 747	214	Missing in isoform Delta.		VSP_005797
Experimental info
	Sequence conflict	301	1	E → D in CAB05388. Ref.1
	Sequence conflict	362	1	P → S in CAB05388. Ref.1
	Sequence conflict	732	1	P → Q in CAB05388. Ref.1
	Sequence conflict	732	1	P → Q in CAB10407. Ref.2
	Sequence conflict	732	1	P → Q in CAB78670. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform Gamma [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 59058999E9152FD8	FASTA	747	81,685
	10 20 30 40 50 60 MNGPPDRVDF KPMGPHHGGS FRPMGFAYDD GFRPMGPNGG VGGEGTRSIV GARYNYPAKY 70 80 90 100 110 120 PPSESPDRRR FIGKAMESDY SVRPTTPPVQ QPLSGQKRGY PISDHGSFTG TDVSDRSSTV 130 140 150 160 170 180 KLFVGSVPRT ATEEEIRPYF EQHGNVLEVA LIKDKRTGQQ QGCCFVKYAT SKDADRAIRA 190 200 210 220 230 240 LHNQITLPGG TGPVQVRYAD GERERIGTLE FKLFVGSLNK QATEKEVEEI FLQFGHVEDV 250 260 270 280 290 300 YLMRDEYRQS RGCGFVKYSS KETAMAAIDG LNGTYTMRGC NQPLIVRFAE PKRPKPGESR 310 320 330 340 350 360 EMAPPVGLGS GPRFQASGPR PTSNFGDSSG DVSHTNPWRP ATSRNVGPPS NTGIRGAGSD 370 380 390 400 410 420 FPPKPGQATL PSNQGGPLGG YGVPPLNPLP VPGVSSSATL QQQNRAAGQH ITPLKKPLHS 430 440 450 460 470 480 PQGLPLPLRP QTNFPGAQAP LQNPYAYSSQ LPTSQLPPQQ NISRATAPQT PLNINLRPTT 490 500 510 520 530 540 VSSATVQFPP RSQQQPLQKM QHPPSELAQL LSQQTQSLQA TFQSSQQAIS QLQQQVQSMQ 550 560 570 580 590 600 QPNQNLPLSQ NGRAGKQQWA GSAIPRVAST TGSTPVSYVQ TAAPAVSQSV GSVKCTWTEH 610 620 630 640 650 660 TSPDGFKYYY NGLTGESKWE KPEEMIVFER EQQKQQQHQE KPTIQQSQTQ LQPLQQQPQQ 670 680 690 700 710 720 VQQQYQGQQL QQPFYSSLYP TPGASHNTQY PSLPVGQNSQ FPMSGIGQNA QDYARTHIPV 730 740 GAASMNDISR TPQSRQSPQE LMWKNKA
	Isoform Alpha. Checksum: B9899EAEF7D364A3	FASTA	505	54,959
	10 20 30 40 50 60 MRDEYRQSRG CGFVKYSSKE TAMAAIDGLN GTYTMRGCNQ PLIVRFAEPK RPKPGESREM 70 80 90 100 110 120 APPVGLGSGP RFQASGPRPT SNFGDSSGDV SHTNPWRPAT SRNVGPPSNT GIRGAGSDFP 130 140 150 160 170 180 PKPGQATLPS NQGGPLGGYG VPPLNPLPVP GVSSSATLQQ QNRAAGQHIT PLKKPLHSPQ 190 200 210 220 230 240 GLPLPLRPQT NFPGAQAPLQ NPYAYSSQLP TSQLPPQQNI SRATAPQTPL NINLRPTTVS 250 260 270 280 290 300 SATVQFPPRS QQQPLQKMQH PPSELAQLLS QQTQSLQATF QSSQQAISQL QQQVQSMQQP 310 320 330 340 350 360 NQNLPLSQNG RAGKQQWAGS AIPRVASTTG STPVSYVQTA APAVSQSVGS VKCTWTEHTS 370 380 390 400 410 420 PDGFKYYYNG LTGESKWEKP EEMIVFEREQ QKQQQHQEKP TIQQSQTQLQ PLQQQPQQVQ 430 440 450 460 470 480 QQYQGQQLQQ PFYSSLYPTP GASHNTQYPS LPVGQNSQFP MSGIGQNAQD YARTHIPVGA 490 500 ASMNDISRTP QSRQSPQELM WKNKA
	Isoform Beta. Checksum: E09FF175F5DA9694	FASTA	260	29,057
	10 20 30 40 50 60 MNGPPDRVDF KPMGPHHGGS FRPMGFAYDD GFRPMGPNGG VGGEGTRSIV GARYNYPAKY 70 80 90 100 110 120 PPSESPDRRR FIGKAMESDY SVRPTTPPVQ QPLSGQKRGY PISDHGSFTG TDVSDRSSTV 130 140 150 160 170 180 KLFVGSVPRT ATEEEIRPYF EQHGNVLEVA LIKDKRTGQQ QGMSISILLG NSRELYFLKF 190 200 210 220 230 240 QVSLKRLMGS NPSLIIGLQL FATIFGLIAY ACQRLICVRF CHRRRLPVLR KGLCTYVGQI 250 260 DFATCVYSRT LDVFEKCRIY
	Isoform Delta. Checksum: AC5ED9020743F2D3	FASTA	533	57,754
	10 20 30 40 50 60 MNGPPDRVDF KPMGPHHGGS FRPMGFAYDD GFRPMGPNGG VGGEGTRSIV GARYNYPAKY 70 80 90 100 110 120 PPSESPDRRR FIGKAMESDY SVRPTTPPVQ QPLSGQKRGY PISDHGSFTG TDVSDRSSTV 130 140 150 160 170 180 KLFVGSVPRT ATEEEIRPYF EQHGNVLEVA LIKDKRTGQQ QGCCFVKYAT SKDADRAIRA 190 200 210 220 230 240 LHNQITLPGG TGPVQVRYAD GERERIGTLE FKLFVGSLNK QATEKEVEEI FLQFGHVEDV 250 260 270 280 290 300 YLMRDEYRQS RGCGFVKYSS KETAMAAIDG LNGTYTMRGC NQPLIVRFAE PKRPKPGESR 310 320 330 340 350 360 EMAPPVGLGS GPRFQASGPR PTSNFGDSSG DVSHTNPWRP ATSRNVGPPS NTGIRGAGSD 370 380 390 400 410 420 FPPKPGQATL PSNQGGPLGG YGVPPLNPLP VPGVSSSATL QQQNRAAGQH ITPLKKPLHS 430 440 450 460 470 480 PQGLPLPLRP QTNFPGAQAP LQNPYAYSSQ LPTSQLPPQQ NISRATAPQT PLNINLRPTT 490 500 510 520 530 VSSATVQFPP RSQQQPLQKM QHPPSELAQL LSQQTQSLQA TFQSSQQAIS QLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"FCA, a gene controlling flowering time in Arabidopsis, encodes a protein containing RNA-binding domains." Macknight R., Bancroft I., Page T., Lister C., Schmidt R., Love K., Westphal L., Murphy G., Sherson S., Cobbett C., Dean C. Cell 89:737-745(1997) [PubMed: 9182761] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING. Strain: cv. Landsberg erecta.
[2]	"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana." Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N. Nature 391:485-488(1998) [PubMed: 9461215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z82989 mRNA. Translation: CAB05388.1. Z82990 mRNA. Translation: CAB05389.1. Z82991 mRNA. Translation: CAB05390.1. Z82992 Genomic DNA. Translation: CAB05391.1. Z82992 Genomic DNA. Translation: CAB05392.1. Z82992 Genomic DNA. Translation: CAB05393.1. Z82993 mRNA. Translation: CAB05394.1. Z82993 mRNA. Translation: CAB05395.1. Z97340 Genomic DNA. Translation: CAB10407.1. Z97340 Genomic DNA. Translation: CAB46035.1. Z97340 Genomic DNA. Translation: CAB46036.1. AL161543 Genomic DNA. Translation: CAB78670.1. AL161543 Genomic DNA. Translation: CAB78671.1. AL161543 Genomic DNA. Translation: CAB78672.1.
IPI	IPI00527912. IPI00531763. IPI00536196. IPI00544871.
PIR	A85181. B85181. E71429.
3D structure databases
SMR	O04425. Positions 118-290, 592-627.
ModBase	Search...
Protein-protein interaction databases
IntAct	O04425. 2 interactions.
STRING	O04425.
Proteomic databases
PRIDE	O04425.
Genome annotation databases
GenomeReviews	Gene locus AT4G16280 in contig CT486007_GR.
Organism-specific databases
TAIR	At4g16280.
Phylogenomic databases
eggNOG	KOG0144.
HOGENOM	HBG633517.
InParanoid	O04425.
PhylomeDB	O04425.
Gene expression databases
Genevestigator	O04425.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR015903. RNP_BRUNO-like. IPR000504. RRM_RNP1. IPR001202. WW_Rsp5_WWP. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.
PANTHER	PTHR10432:SF35. RNP_BRUNO-like. 1 hit.
Pfam	PF00076. RRM_1. 2 hits. PF00397. WW. 1 hit. [Graphical view]
SMART	SM00360. RRM. 2 hits. SM00456. WW. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 2 hits. PS01159. WW_DOMAIN_1. False negative. PS50020. WW_DOMAIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FCA_ARATH

Accession

Primary (citable) accession number: O04425
Secondary accession number(s): O04424, O04727, O23475

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	February 9, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)