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Protein

Uricase

Gene

At2g26230

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.

Catalytic activityi

Urate + O2 + H2O = 5-hydroxyisourate + H2O2.

Pathwayi: urate degradation

This protein is involved in step 1 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (At2g26230)
  2. Uric acid degradation bifunctional protein TTL (TTL)
  3. Uric acid degradation bifunctional protein TTL (TTL)
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei182 – 1821Charge relay systemBy similarity
Binding sitei182 – 1821SubstrateBy similarity
Active sitei238 – 2381Charge relay systemBy similarity

GO - Molecular functioni

  • urate oxidase activity Source: TAIR

GO - Biological processi

  • peroxisome organization Source: TAIR
  • purine nucleobase metabolic process Source: UniProtKB-KW
  • urate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism

Enzyme and pathway databases

BioCyciARA:AT2G26230-MONOMER.
UniPathwayiUPA00394; UER00650.

Names & Taxonomyi

Protein namesi
Recommended name:
Uricase (EC:1.7.3.3)
Alternative name(s):
Nodulin-35 homolog
Urate oxidase
Gene namesi
Ordered Locus Names:At2g26230
ORF Names:T1D16.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G26230.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 309308UricasePRO_0000165999Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO04420.
PRIDEiO04420.

Expressioni

Gene expression databases

GenevisibleiO04420. AT.

Interactioni

Protein-protein interaction databases

BioGridi2515. 2 interactions.
STRINGi3702.AT2G26230.1.

Structurei

3D structure databases

ProteinModelPortaliO04420.
SMRiO04420. Positions 14-304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 642Substrate bindingBy similarity
Regioni237 – 2382Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi307 – 3093Microbody targeting signalSequence analysis

Sequence similaritiesi

Belongs to the uricase family.Curated

Phylogenomic databases

eggNOGiKOG1599. Eukaryota.
COG3648. LUCA.
HOGENOMiHOG000250659.
InParanoidiO04420.
KOiK00365.
OMAiKDRCFAT.
PhylomeDBiO04420.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQEADGIRL DQRHGKARVR VGRVWRHAHD GSHHFVEWNV SISLLSHCLS
60 70 80 90 100
SYRLDDNSDI VATDTIKNTV YVKAKECGDR LSVEEFAILI GKHFCSFYPQ
110 120 130 140 150
VFTAIVNIIE KPWERVSIDG KPHLHGFKLG SENHTTEARV EKSGALNLTS
160 170 180 190 200
GIGGLALLKT TQSGFERFVR DKYTILPETR ERMLATEVNA SWRYSYESVA
210 220 230 240 250
SIPTKGLYFS EKFMDVKKVL MDTFFGPPET GVYSPSVQRT LYLMGSAVLK
260 270 280 290 300
RFADVSSIHL KMPNIHFLPV NLSTKENPSM VKFKDDVYLP TDEPHGSIEA

TLSRITSKL
Length:309
Mass (Da):34,881
Last modified:February 5, 2008 - v2
Checksum:i9DB8FAD58FDF1404
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111D → E in CAA72005 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11120 mRNA. Translation: CAA72005.1.
AC004484 Genomic DNA. Translation: AAC14527.1.
CP002685 Genomic DNA. Translation: AEC07811.1.
AY050318 mRNA. Translation: AAK91335.1.
AY101530 mRNA. Translation: AAM26651.1.
AY087805 mRNA. Translation: AAM65341.1.
PIRiH84657.
RefSeqiNP_180191.1. NM_128180.4.
UniGeneiAt.5406.

Genome annotation databases

EnsemblPlantsiAT2G26230.1; AT2G26230.1; AT2G26230.
GeneIDi817163.
GrameneiAT2G26230.1; AT2G26230.1; AT2G26230.
KEGGiath:AT2G26230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11120 mRNA. Translation: CAA72005.1.
AC004484 Genomic DNA. Translation: AAC14527.1.
CP002685 Genomic DNA. Translation: AEC07811.1.
AY050318 mRNA. Translation: AAK91335.1.
AY101530 mRNA. Translation: AAM26651.1.
AY087805 mRNA. Translation: AAM65341.1.
PIRiH84657.
RefSeqiNP_180191.1. NM_128180.4.
UniGeneiAt.5406.

3D structure databases

ProteinModelPortaliO04420.
SMRiO04420. Positions 14-304.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2515. 2 interactions.
STRINGi3702.AT2G26230.1.

Proteomic databases

PaxDbiO04420.
PRIDEiO04420.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G26230.1; AT2G26230.1; AT2G26230.
GeneIDi817163.
GrameneiAT2G26230.1; AT2G26230.1; AT2G26230.
KEGGiath:AT2G26230.

Organism-specific databases

TAIRiAT2G26230.

Phylogenomic databases

eggNOGiKOG1599. Eukaryota.
COG3648. LUCA.
HOGENOMiHOG000250659.
InParanoidiO04420.
KOiK00365.
OMAiKDRCFAT.
PhylomeDBiO04420.

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650.
BioCyciARA:AT2G26230-MONOMER.

Miscellaneous databases

PROiO04420.

Gene expression databases

GenevisibleiO04420. AT.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A nodulin-35 homologue is encoded in the Arabidopsis genome."
    Marchfelder A., Binder S., Brennicke A.
    Trends Plant Sci. 2:167-168(1997)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Landsberg erecta.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiURIC_ARATH
AccessioniPrimary (citable) accession number: O04420
Secondary accession number(s): O64848, Q94A71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 5, 2008
Last modified: February 17, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.