Reviewed, UniProtKB/Swiss-Prot O04420 (URIC_ARATH)

Names and origin

Protein names

Recommended name:
    Uricase
    EC=1.7.3.3
Alternative name(s):
    Urate oxidase
    Nodulin-35 homolog

Gene names

Ordered Locus Names:	At2g26230
ORF Names:	T1D16.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which spontaneously decomposes to form allantoin.
Catalytic activity	Urate + O2 + H2O = 5-hydroxyisourate + H2O2.
Pathway	Purine metabolism; uric acid degradation; (S)-allantoin from uric acid: step 1/3.
Subcellular location	Peroxisome Potential.
Sequence similarities	Belongs to the uricase family.

Ontologies

Keywords
Biological process	Purine metabolism
Cellular component	Peroxisome
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from direct assay. Source: TAIR
Molecular function	urate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Uricase		PRO_0000165999
Regions
	Region	237 – 238	2	Substrate binding By similarity
	Motif	307 – 309	3	Microbody targeting signal Potential
Sites
	Active site	182	1	Charge relay system By similarity
	Active site	238	1	Charge relay system By similarity
	Binding site	63	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity
Experimental info
	Sequence conflict	11	1	D → E in CAA72005. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O04420-1 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: 9DB8FAD58FDF1404	FASTA	309	34,881
	10 20 30 40 50 60 MAQEADGIRL DQRHGKARVR VGRVWRHAHD GSHHFVEWNV SISLLSHCLS SYRLDDNSDI 70 80 90 100 110 120 VATDTIKNTV YVKAKECGDR LSVEEFAILI GKHFCSFYPQ VFTAIVNIIE KPWERVSIDG 130 140 150 160 170 180 KPHLHGFKLG SENHTTEARV EKSGALNLTS GIGGLALLKT TQSGFERFVR DKYTILPETR 190 200 210 220 230 240 ERMLATEVNA SWRYSYESVA SIPTKGLYFS EKFMDVKKVL MDTFFGPPET GVYSPSVQRT 250 260 270 280 290 300 LYLMGSAVLK RFADVSSIHL KMPNIHFLPV NLSTKENPSM VKFKDDVYLP TDEPHGSIEA TLSRITSKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A nodulin-35 homologue is encoded in the Arabidopsis genome." Marchfelder A., Binder S., Brennicke A. Trends Plant Sci. 2:167-168(1997) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y11120 mRNA. Translation: CAA72005.1. AC004484 Genomic DNA. Translation: AAC14527.1. AY050318 mRNA. Translation: AAK91335.1. AY101530 mRNA. Translation: AAM26651.1. AY087805 mRNA. Translation: AAM65341.1.
IPI	IPI00518603.
PIR	H84657.
RefSeq	NP_180191.1.
UniGene	At.5406
3D structure databases
HSSP	HSSP built from PDB template 1UOX based on UniProtKB Q00511.
ModBase	Search...
Proteomic databases
PRIDE	O04420.
ProMEX	O04420.
Genome annotation databases
GeneID	817163.
GenomeReviews	Gene locus AT2G26230 in contig CT485783_GR.
KEGG	ath:AT2G26230.
Organism-specific databases
TAIR	At2g26230.
Phylogenomic databases
OMA	O04420. KTTQSGF.
Enzyme and pathway databases
BRENDA	1.7.3.3. 302.
Gene expression databases
ArrayExpress	O04420.
Family and domain databases
InterPro	IPR002042. Uricase. IPR019842. Uricase_CS. [Graphical view]
Gene3D	G3DSA:3.10.270.10. Uricase. 1 hit.
PANTHER	PTHR10395:SF1. Uricase. 1 hit.
Pfam	PF01014. Uricase. 2 hits. [Graphical view]
PRINTS	PR00093. URICASE.
ProDom	PD003367. Uricase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03383. urate_oxi. 1 hit.
PROSITE	PS00366. URICASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URIC_ARATH

Accession

Primary (citable) accession number: O04420
Secondary accession number(s): O64848, Q94A71

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 5, 2008
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)