O04408 (KSA_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ent-copalyl diphosphate synthase, chloroplastic Short name=Ent-CDP synthase EC=5.5.1.13 Alternative name(s): Ent-copalyl diphosphate synthase Ent-kaurene synthase A Short name=KSA
Organism	Pisum sativum (Garden pea)
Taxonomic identifier	3888 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	801 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the conversion of geranylgeranyl diphosphate to the gibberellin precursor ent-copalyl diphosphate.
Catalytic activity	Geranylgeranyl diphosphate = ent-copalyl diphosphate.
Cofactor	Magnesium Potential.
Pathway	Plant hormone biosynthesis; gibberellin biosynthesis.
Subcellular location	Plastid › chloroplast.
Domain	The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similarities	Belongs to the terpene synthase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
Gene Ontology (GO)
Biological_process	gibberellin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ent-copalyl diphosphate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro terpene synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 801		Ent-copalyl diphosphate synthase, chloroplastic		PRO_0000033624
Regions
	Motif	373 – 376	4	DXDD motif

Sequences

	Sequence		Length	Mass (Da)
	O04408 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 5CB88ADE00366844	FASTA	801	92,718
	10 20 30 40 50 60 MFTHFSTHFH LPSSSSLFFL HPFYKSSSLG AVSFVAKDKE KRCRAISKSR TQEYEGVFQT 70 80 90 100 110 120 NVATLKLSEI NVEDVIVIDD EEEQDIRVGL VNKIKSILSS LEDGEITISA YDTAWVALVE 130 140 150 160 170 180 DVNAISTPQF PSSLEWIAKN QLQDGSWGDS RLFSAHDRII NTLACVIALR SWNMHSEKCD 190 200 210 220 230 240 KGMIFFRENL SKLENENEEH MPIGFEVAFP SLLEGARGIK PLMCPNDSPI LKNIFEKRDE 250 260 270 280 290 300 KLTRIPKEIM HKVPTTLLHS LEGMSGLDWK QLLKLQSQDG SFLFSPSSTA FALMQTKDGN 310 320 330 340 350 360 CLKYLNNVVK KFNGGVPNVY PVDLFEHIWV VDRLERLGIS RFFRHEIKDC MNYVSKIWSE 370 380 390 400 410 420 KGICWARNSN VQDIDDTAMA FRLLRLHGHQ VSAHVFKHFE RNGEFFCFAG QCTQAVTGMY 430 440 450 460 470 480 NLFRASQVLF PGEKILEHAK HFSAKVLKEK REANELIDKW IIMKNLPEEV GYALDMPWYA 490 500 510 520 530 540 NLDRIETRFY IDQYGAESDV WIGKTLYRMA YVNNNNYLEL AKLDYNNCQA QHLIEWNVIQ 550 560 570 580 590 600 TWYLESRLGE FGLSKRDLLL AYFLATGSIF EPERSHERLA WAKTTALLET IKCYVRNEDL 610 620 630 640 650 660 RKDFAKKFND HIDVRDYSIA RRMKRNKTEH ELVESLFATI GEISWDVRLS YGHEIGYDMH 670 680 690 700 710 720 QCWKKWLSSW QSEGDKCEGE AELLIQIINL CSNHWISEGP SMQSTIQHLL QLTNSICHKL 730 740 750 760 770 780 SCYQKDKELK GISCQENITN SEVESKMQEL VQMVFQKCPN DIDFNVKNTF FTIAKSFYYA 790 800 AFCDSRTINF HIAKVLFEKV V

References

[1]	"The LS locus of pea encodes the gibberellin biosynthesis enzyme ent-kaurene synthase A." Ait-Ali T., Swain S.M., Reid J.B., Sun T.-P., Kamiya Y. Plant J. 11:443-454(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U63652 mRNA. Translation: AAB58822.1.
PIR	T06783.
3D structure databases
ProteinModelPortal	O04408.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00390.
Family and domain databases
Gene3D	1.10.600.10. 1 hit. 1.50.30.10. 1 hit.
InterPro	IPR001906. Terpene_synth_N. IPR005630. Terpene_synthase_metal-bd. IPR008930. Terpenoid_cyclase/PrenylTrfase. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF01397. Terpene_synth. 1 hit. PF03936. Terpene_synth_C. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 1 hit. SSF48576. Terpenoid_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KSA_PEA

Accession

Primary (citable) accession number: O04408

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program