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O04408 (KSA_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ent-copalyl diphosphate synthase, chloroplastic

Short name=Ent-CDP synthase
EC=5.5.1.13
Alternative name(s):
Ent-copalyl diphosphate synthase
Ent-kaurene synthase A
Short name=KSA
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length801 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the conversion of geranylgeranyl diphosphate to the gibberellin precursor ent-copalyl diphosphate.

Catalytic activity

Geranylgeranyl diphosphate = ent-copalyl diphosphate.

Cofactor

Magnesium Potential.

Pathway

Plant hormone biosynthesis; gibberellin biosynthesis.

Subcellular location

Plastidchloroplast.

Domain

The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similarities

Belongs to the terpene synthase family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandMagnesium
Metal-binding
   Molecular functionIsomerase
Gene Ontology (GO)
   Biological_processgibberellin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionent-copalyl diphosphate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

terpene synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 801Ent-copalyl diphosphate synthase, chloroplasticPRO_0000033624

Regions

Motif373 – 3764DXDD motif

Sequences

Sequence LengthMass (Da)Tools
O04408 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 5CB88ADE00366844

FASTA80192,718
        10         20         30         40         50         60 
MFTHFSTHFH LPSSSSLFFL HPFYKSSSLG AVSFVAKDKE KRCRAISKSR TQEYEGVFQT 

        70         80         90        100        110        120 
NVATLKLSEI NVEDVIVIDD EEEQDIRVGL VNKIKSILSS LEDGEITISA YDTAWVALVE 

       130        140        150        160        170        180 
DVNAISTPQF PSSLEWIAKN QLQDGSWGDS RLFSAHDRII NTLACVIALR SWNMHSEKCD 

       190        200        210        220        230        240 
KGMIFFRENL SKLENENEEH MPIGFEVAFP SLLEGARGIK PLMCPNDSPI LKNIFEKRDE 

       250        260        270        280        290        300 
KLTRIPKEIM HKVPTTLLHS LEGMSGLDWK QLLKLQSQDG SFLFSPSSTA FALMQTKDGN 

       310        320        330        340        350        360 
CLKYLNNVVK KFNGGVPNVY PVDLFEHIWV VDRLERLGIS RFFRHEIKDC MNYVSKIWSE 

       370        380        390        400        410        420 
KGICWARNSN VQDIDDTAMA FRLLRLHGHQ VSAHVFKHFE RNGEFFCFAG QCTQAVTGMY 

       430        440        450        460        470        480 
NLFRASQVLF PGEKILEHAK HFSAKVLKEK REANELIDKW IIMKNLPEEV GYALDMPWYA 

       490        500        510        520        530        540 
NLDRIETRFY IDQYGAESDV WIGKTLYRMA YVNNNNYLEL AKLDYNNCQA QHLIEWNVIQ 

       550        560        570        580        590        600 
TWYLESRLGE FGLSKRDLLL AYFLATGSIF EPERSHERLA WAKTTALLET IKCYVRNEDL 

       610        620        630        640        650        660 
RKDFAKKFND HIDVRDYSIA RRMKRNKTEH ELVESLFATI GEISWDVRLS YGHEIGYDMH 

       670        680        690        700        710        720 
QCWKKWLSSW QSEGDKCEGE AELLIQIINL CSNHWISEGP SMQSTIQHLL QLTNSICHKL 

       730        740        750        760        770        780 
SCYQKDKELK GISCQENITN SEVESKMQEL VQMVFQKCPN DIDFNVKNTF FTIAKSFYYA 

       790        800 
AFCDSRTINF HIAKVLFEKV V 

« Hide

References

[1]"The LS locus of pea encodes the gibberellin biosynthesis enzyme ent-kaurene synthase A."
Ait-Ali T., Swain S.M., Reid J.B., Sun T.-P., Kamiya Y.
Plant J. 11:443-454(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U63652 mRNA. Translation: AAB58822.1.
PIRT06783.

3D structure databases

ProteinModelPortalO04408.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00390.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 2 hits.
SSF48576. SSF48576. 1 hit.
ProtoNetSearch...

Entry information

Entry nameKSA_PEA
AccessionPrimary (citable) accession number: O04408
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways