Reviewed, UniProtKB/Swiss-Prot O04397 (FENR2_TOBAC)

Names and origin

Protein names	Recommended name:     Ferredoxin--NADP reductase, root-type isozyme, chloroplastic       Short name=FNR     EC=1.18.1.2
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power. Is involved in nitrate assimilation.
Catalytic activity	2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.
Cofactor	FAD.
Pathway	Energy metabolism; photosynthesis.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the ferredoxin--NADP reductase type 1 family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid membrane Inferred from electronic annotation. Source: InterPro
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin-NADP+ reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 60	60	Chloroplast Potential
	Chain	61 – 375	315	Ferredoxin--NADP reductase, root-type isozyme, chloroplastic		PRO_0000019416
Regions
	Domain	91 – 219	129	FAD-binding FR-type
	Nucleotide binding	151 – 154	4	FAD By similarity
	Nucleotide binding	172 – 174	3	FAD By similarity
	Nucleotide binding	193 – 195	3	FAD By similarity
	Nucleotide binding	266 – 267	2	NADP By similarity
	Nucleotide binding	296 – 297	2	NADP By similarity
	Nucleotide binding	334 – 335	2	NADP By similarity
Sites
	Binding site	174	1	NADP By similarity
	Binding site	178	1	FAD By similarity
	Binding site	235	1	FAD By similarity
	Binding site	235	1	NADP; via amide nitrogen By similarity
	Binding site	306	1	NADP By similarity
	Binding site	373	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O04397-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: AB5ED3988F746B92	FASTA	375	41,958
	10 20 30 40 50 60 MAHSALSQVS VAVPLQTDSS FRRSTFKATS ITFSDRSSWI SMPPIDLKAA PSRNQHIVCM 70 80 90 100 110 120 SVQQASKAKV SVSPLSLEDA KEPPLNIYKP KEPYTATIVS VERLVGPKAP GETCHIVIDH 130 140 150 160 170 180 DGNLPYWEGQ SYGVIPPGEN PKKPGNPHNV RLYLIASTRY GDSFDGKTAS LCVRRAVYYD 190 200 210 220 230 240 PETGKEDPSK NGVCSNFLCD SKPGDKVKIT GPSGKIMLLP EEIPNATHIM IGTGTGVAPF 250 260 270 280 290 300 RGYLRRMFME SVPTKFNGLA WLFLGVANTD SLLYDDEFTK YLNDYPGNFR YDRALSREQK 310 320 330 340 350 360 NNKGGKMYVQ DKIEEYSDEI FKLLDEGAHI YFCGLKGMMP GIQDTLKRVA ERRGESWEQK 370 LSQLKKNKQW HVEVY

References

[1]	"Nucleotide sequence of a ferredoxin-NADP+ oxidoreductase cDNA from cultured tobacco cells." Kumada H.O., Aoki H., Doyama N., Ida S. Plant Gene Register PGR98-038 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Bright Yellow 2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB004307 mRNA. Translation: BAA20365.1.
PIR	T02215.
3D structure databases
HSSP	HSSP built from PDB template 1JB9 based on UniProtKB Q41736.
SMR	O04397. Positions 66-375.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.18.1.2. 298.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR012146. Frd-NADP+_RD. IPR015701. FRD_Red. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
PANTHER	PTHR19384:SF1. FRD_Red. 1 hit.
Pfam	PF00175. NAD_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF000361. Frd-NADP+_RD. 1 hit.
PRINTS	PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FENR2_TOBAC

Accession

Primary (citable) accession number: O04397

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)