Reviewed, UniProtKB/Swiss-Prot O04395 (FLS_MATIN)

Names and origin

Protein names	Recommended name:     Flavonol synthase/flavanone 3-hydroxylase       Short name=FLS     EC=1.14.11.23     EC=1.14.11.9
Organism	Matthiola incana (Common stock)
Taxonomic identifier	3724 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Matthiola

Protein attributes

Sequence length	291 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin.
Catalytic activity	A dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O. A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2.
Cofactor	Binds 1 ascorbate molecule per subunit. Binds 1 iron ion per subunit.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW flavonol synthase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW naringenin 3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 291	›291	Flavonol synthase/flavanone 3-hydroxylase		PRO_0000067295
Regions
	Domain	151 – 250	100	Fe2OG dioxygenase
Sites
	Metal binding	175	1	Iron By similarity
	Metal binding	177	1	Iron By similarity
	Metal binding	231	1	Iron By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	O04395-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 6B8E4E3D2834720A	FASTA	291	33,430
	10 20 30 40 50 60 QVPVVDLSCP DEELVARTVV KASEDWGVFQ VVNHGIPTEL IQRLQKVGRE FFELPEAEKR 70 80 90 100 110 120 SCAREAGSVE GYGRRIELDI KKRKGIVDQI YLSTWPPSSV NYRYWPKSPP DYREVNEEYA 130 140 150 160 170 180 RHVKTLSEKI MEWLSEGLGL GREAIKEVNG CWYVMNINHY PPYPHSDSFN GLEPHTDING 190 200 210 220 230 240 LTLIITNEIP GLQVFKDDHW IEVEYIPSAI IVNIGDQIMM LSNGKYKNVL HKTTVDKEKT 250 260 270 280 290 RMSWPVLVSP TYDMVVGPLP ELTSEDDPPK FKPIAYKDYV HNKITFLKNK S

References

[1]	"Cloning and expression of a flavonol synthase gene from common stock (Matthiola incana)." Henkel J., Forkmann G. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Flower bud and Petal.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF001391 mRNA. Translation: AAB58800.1.
3D structure databases
SMR	O04395. Positions 1-284.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.11.23. 228993. 1.14.11.9. 228993.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FLS_MATIN

Accession

Primary (citable) accession number: O04395

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 1, 1997
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)