Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein argonaute 1

Gene

AGO1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Requires DRB1 for directional loading of the small RNA duplex (guide stand and passenger strand) onto RISC for passenger strand degradation. Unlike animal RISC that associates in high molecular weight complex, Arabidopsis RISC is probably composed only of the AGO1 protein and associated RNA without any other proteins. Associates mainly with miRNAs of 21 nucleotide in length and preferentially recruits small RNAs with a 5' terminal uridine. Associates with 22 nucleotide miRNAs to trigger RDR6-dependent secondary siRNAs biogenesis. This pathway amplifies silencing by using the target RNA as substrate to generate secondary siRNAs. Binds to miR168 which targets its own mRNA for repression, establishing a homeostatic regulatory loop. Involved in antiviral RNA silencing by contributing to viral RNA clearance. Is capable of targeting viral RNAs with more compact structures than AGO7 which favors less structured RNA targets. May not associate with 24 nucleotide siRNAs involved in chromatin silencing. Essential for multiple processes in development. Essential for proper development of leaves and floral organs, and formation of axillary meristems. Like AGO10, required for stem cell function and organ polarity.11 Publications

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi760Divalent metal cationBy similarity1
Metal bindingi846Divalent metal cationBy similarity1
Metal bindingi986Divalent metal cationBy similarity1

GO - Molecular functioni

  • endoribonuclease activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • miRNA binding Source: CAFA
  • mRNA binding Source: TAIR

GO - Biological processi

  • adaxial/abaxial pattern specification Source: TAIR
  • adventitious root development Source: TAIR
  • auxin metabolic process Source: TAIR
  • defense response to virus Source: TAIR
  • gene silencing by miRNA Source: TAIR
  • innate immune response Source: TAIR
  • leaf morphogenesis Source: TAIR
  • leaf proximal/distal pattern formation Source: TAIR
  • leaf vascular tissue pattern formation Source: TAIR
  • posttranscriptional gene silencing Source: TAIR
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to auxin Source: TAIR
  • response to far red light Source: TAIR
  • stem cell development Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • viral gene silencing in virus induced gene silencing Source: CACAO
  • viral process Source: UniProtKB-KW
  • virus induced gene silencing Source: TAIR

Keywordsi

Molecular functionDevelopmental protein, Repressor, Ribonucleoprotein, RNA-binding
Biological processHost-virus interaction, Plant defense, RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-ATH-426486 Small interfering RNA (siRNA) biogenesis
R-ATH-426496 Post-transcriptional silencing by small RNAs
R-ATH-5578749 Transcriptional regulation by small RNAs

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute 1
Gene namesi
Name:AGO1
Ordered Locus Names:At1g48410
ORF Names:F11A17.3, T1N15.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G48410
TAIRilocus:2007760 AT1G48410

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Narrow rosette leaves. Single shoot bearing a terminal inflorescence. Abnormal inflorescence with infertile flowers and filamentous organs.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940681 – 1048Protein argonaute 1Add BLAST1048

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1001PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO04379
PRIDEiO04379

PTM databases

iPTMnetiO04379

Expressioni

Tissue specificityi

Widely expressed at low levels.

Developmental stagei

Expressed throughout all developmental stages.

Gene expression databases

ExpressionAtlasiO04379 baseline and differential
GenevisibleiO04379 AT

Interactioni

Subunit structurei

Interacts with turnip yellows virus (TuYV) protein P0 (via F-box-like domain); this interaction targets AGO1 for degradation, and thereby suppresses its function in silencing. Interacts with turnip crinkle virus (TCV) capsid protein P38 (via GW motifs); these interactions inhibit RNA silencing ability of AGO1. Interacts (via PAZ domain) with cucumber mosaic virus (CMV) protein 2b; these interactions inhibit AGO1 function in RNA silencing. Interacts with SDE3 (PubMed:17158744, PubMed:17869110, PubMed:20439431, PubMed:22850669, PubMed:22940249). Interacts with HESO1 (PubMed:24733911). Interacts with URT1 (PubMed:25928341).7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AMP1Q9M1S82EBI-6912745,EBI-6912802

Protein-protein interaction databases

BioGridi26487, 6 interactors
IntActiO04379, 1 interactor
STRINGi3702.AT1G48410.2

Structurei

Secondary structure

11048
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi596 – 598Combined sources3
Beta strandi604 – 609Combined sources6
Helixi616 – 632Combined sources17
Helixi650 – 652Combined sources3
Helixi653 – 666Combined sources14
Beta strandi676 – 681Combined sources6
Helixi688 – 698Combined sources11
Beta strandi704 – 708Combined sources5
Helixi709 – 714Combined sources6
Helixi717 – 730Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VNAX-ray2.00A592-739[»]
3VNBX-ray1.50A592-739[»]
4G0PX-ray1.80A593-738[»]
4G0QX-ray1.80A593-738[»]
4G0XX-ray1.35A593-738[»]
4G0YX-ray1.65A593-738[»]
4G0ZX-ray1.75A593-738[»]
ProteinModelPortaliO04379
SMRiO04379
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini391 – 501PAZPROSITE-ProRule annotationAdd BLAST111
Domaini676 – 997PiwiPROSITE-ProRule annotationAdd BLAST322

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni886 – 887Interaction with guide RNASequence analysis2
Regioni932 – 940Interaction with guide RNASequence analysis9
Regioni969 – 991Interaction with guide RNASequence analysisAdd BLAST23

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 104Gly-richAdd BLAST92

Sequence similaritiesi

Belongs to the argonaute family. Ago subfamily.Curated

Phylogenomic databases

eggNOGiKOG1041 Eukaryota
ENOG410XP07 LUCA
HOGENOMiHOG000116043
InParanoidiO04379
KOiK11593
OMAiIQWVSVV
PhylomeDBiO04379

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR014811 ArgoL1
IPR032472 ArgoL2
IPR024357 Argonaut_Gly-rich
IPR032473 Argonaute_Mid_dom
IPR032474 Argonaute_N
IPR003100 PAZ_dom
IPR036085 PAZ_dom_sf
IPR003165 Piwi
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF08699 ArgoL1, 1 hit
PF16488 ArgoL2, 1 hit
PF16487 ArgoMid, 1 hit
PF16486 ArgoN, 1 hit
PF12764 Gly-rich_Ago1, 1 hit
PF02170 PAZ, 1 hit
PF02171 Piwi, 1 hit
SMARTiView protein in SMART
SM01163 DUF1785, 1 hit
SM00949 PAZ, 1 hit
SM00950 Piwi, 1 hit
SUPFAMiSSF101690 SSF101690, 2 hits
SSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS50821 PAZ, 1 hit
PS50822 PIWI, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O04379-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVRKRRTDAP SEGGEGSGSR EAGPVSGGGR GSQRGGFQQG GGQHQGGRGY
60 70 80 90 100
TPQPQQGGRG GRGYGQPPQQ QQQYGGPQEY QGRGRGGPPH QGGRGGYGGG
110 120 130 140 150
RGGGPSSGPP QRQSVPELHQ ATSPTYQAVS SQPTLSEVSP TQVPEPTVLA
160 170 180 190 200
QQFEQLSVEQ GAPSQAIQPI PSSSKAFKFP MRPGKGQSGK RCIVKANHFF
210 220 230 240 250
AELPDKDLHH YDVTITPEVT SRGVNRAVMK QLVDNYRDSH LGSRLPAYDG
260 270 280 290 300
RKSLYTAGPL PFNSKEFRIN LLDEEVGAGG QRREREFKVV IKLVARADLH
310 320 330 340 350
HLGMFLEGKQ SDAPQEALQV LDIVLRELPT SRYIPVGRSF YSPDIGKKQS
360 370 380 390 400
LGDGLESWRG FYQSIRPTQM GLSLNIDMSS TAFIEANPVI QFVCDLLNRD
410 420 430 440 450
ISSRPLSDAD RVKIKKALRG VKVEVTHRGN MRRKYRISGL TAVATRELTF
460 470 480 490 500
PVDERNTQKS VVEYFHETYG FRIQHTQLPC LQVGNSNRPN YLPMEVCKIV
510 520 530 540 550
EGQRYSKRLN ERQITALLKV TCQRPIDREK DILQTVQLND YAKDNYAQEF
560 570 580 590 600
GIKISTSLAS VEARILPPPW LKYHESGREG TCLPQVGQWN MMNKKMINGG
610 620 630 640 650
TVNNWICINF SRQVQDNLAR TFCQELAQMC YVSGMAFNPE PVLPPVSARP
660 670 680 690 700
EQVEKVLKTR YHDATSKLSQ GKEIDLLIVI LPDNNGSLYG DLKRICETEL
710 720 730 740 750
GIVSQCCLTK HVFKMSKQYM ANVALKINVK VGGRNTVLVD ALSRRIPLVS
760 770 780 790 800
DRPTIIFGAD VTHPHPGEDS SPSIAAVVAS QDWPEITKYA GLVCAQAHRQ
810 820 830 840 850
ELIQDLFKEW KDPQKGVVTG GMIKELLIAF RRSTGHKPLR IIFYRDGVSE
860 870 880 890 900
GQFYQVLLYE LDAIRKACAS LEAGYQPPVT FVVVQKRHHT RLFAQNHNDR
910 920 930 940 950
HSVDRSGNIL PGTVVDSKIC HPTEFDFYLC SHAGIQGTSR PAHYHVLWDE
960 970 980 990 1000
NNFTADGLQS LTNNLCYTYA RCTRSVSIVP PAYYAHLAAF RARFYMEPET
1010 1020 1030 1040
SDSGSMASGS MARGGGMAGR STRGPNVNAA VRPLPALKEN VKRVMFYC
Length:1,048
Mass (Da):116,191
Last modified:July 1, 1997 - v1
Checksum:i3E5146343A09C541
GO
Isoform 2 (identifier: O04379-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     332-332: R → RIR

Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:1,050
Mass (Da):116,460
Checksum:i233B89E5E4D9E1EC
GO

Sequence cautioni

The sequence AAF79718 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040612332R → RIR in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91995 mRNA Translation: AAC18440.1
AC007932 Genomic DNA Translation: AAD49755.1
AC020889 Genomic DNA Translation: AAF79718.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE32287.1
CP002684 Genomic DNA Translation: AEE32288.1
CP002684 Genomic DNA Translation: AEE32289.1
BT000941 mRNA Translation: AAN41341.1
AK227868 mRNA Translation: BAE99844.1
RefSeqiNP_001185169.1, NM_001198240.1 [O04379-2]
NP_175274.1, NM_103737.4 [O04379-1]
NP_849784.1, NM_179453.3 [O04379-2]
UniGeneiAt.21124

Genome annotation databases

EnsemblPlantsiAT1G48410.1; AT1G48410.1; AT1G48410 [O04379-1]
AT1G48410.2; AT1G48410.2; AT1G48410 [O04379-2]
AT1G48410.3; AT1G48410.3; AT1G48410 [O04379-2]
GeneIDi841262
GrameneiAT1G48410.1; AT1G48410.1; AT1G48410 [O04379-1]
AT1G48410.2; AT1G48410.2; AT1G48410 [O04379-2]
AT1G48410.3; AT1G48410.3; AT1G48410 [O04379-2]
KEGGiath:AT1G48410

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAGO1_ARATH
AccessioniPrimary (citable) accession number: O04379
Secondary accession number(s): Q0WSQ4, Q3ECU7, Q9LP83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health