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O04373 (ILL4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
IAA-amino acid hydrolase ILR1-like 4

EC=3.5.1.-
Gene names
Name:ILL4
Synonyms:IAR3, JR3
Ordered Locus Names:At1g51760
ORF Names:F19C24.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Ala.

Cofactor

Manganese. The ion enhances activity.

Subcellular location

Endoplasmic reticulum lumen Potential.

Tissue specificity

Expressed most strongly in roots, stems, cauline leaves and flowers. Ref.2

Induction

By jasmonic acid (JA) and by wounding. Ref.1

Sequence similarities

Belongs to the peptidase M20 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 440417IAA-amino acid hydrolase ILR1-like 4
PRO_0000045470

Regions

Motif437 – 4404Prevents secretion from ER Potential

Experimental info

Mutagenesis2061S → L in iar3-1; reduces activity. Ref.2
Mutagenesis2241G → E in iar3-2; abolishes activity. Ref.2
Mutagenesis2261G → E in iar3-4; reduces activity. Ref.2
Mutagenesis2301A → T in iar3-3; reduces activity. Ref.2
Sequence conflict2111M → I in CAA73905. Ref.1
Sequence conflict2911A → AFST in CAA73905. Ref.1
Sequence conflict325 – 3295EEEKP → ARGET in CAA73905. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O04373 [UniParc].

Last modified January 10, 2006. Version 2.
Checksum: 8B605EEF67A1746E

FASTA44048,263
        10         20         30         40         50         60 
MSFFKWVSFV LILHLLNPTL ISCSSNGLSQ IPSKFLTLAK RNDFFDWMVG IRRRIHENPE 

        70         80         90        100        110        120 
LGYEEVETSK LVRAELEKMG VSYKYPVAVT GVVGYVGTGH APFVALRADM DALAMQEMVE 

       130        140        150        160        170        180 
WEHKSKVPGK MHACGHDAHT TMLLGAAKLL KEHEEELQGT VVLVFQPAEE GGGGAKKIVE 

       190        200        210        220        230        240 
AGVLENVSAI FGLHVTNQLA LGQVSSREGP MLAGSGFFKA KISGKGGHAA LPQHTIDPIL 

       250        260        270        280        290        300 
AASNVIVSLQ HLVSREADPL DSQVVTVAKF EGGGAFNVIP DSVTIGGTFR AFSTKSFMQL 

       310        320        330        340        350        360 
KKRIEQVITR QASVNMCNAT VDFIEEEKPF FPPTVNDKAL HQFFKNVSGD MLGIENYVEM 

       370        380        390        400        410        420 
QPLMGSEDFS FYQQAIPGHF SFVGMQNKAR SPMASPHSPY FEVNEELLPY GASLHASMAT 

       430        440 
RYLLELKAST LNKSNKKDEL 

« Hide

References

« Hide 'large scale' references
[1]"Jasmonic acid-dependent and -independent signaling pathways control wound-induced gene activation in Arabidopsis thaliana."
Titarenko E., Rojo E., Leon J., Sanchez-Serrano J.J.
Plant Physiol. 115:817-826(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
[2]"IAR3 encodes an auxin conjugate hydrolase from Arabidopsis."
Davies R.T., Goetz D.H., Lasswell J.E., Anderson M.N., Bartel B.
Plant Cell 11:365-376(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, MUTAGENESIS OF SER-206; GLY-224; GLY-226 AND ALA-230.
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Characterization of a family of IAA-amino acid conjugate hydrolases from Arabidopsis."
LeClere S., Tellez R., Rampey R.A., Matsuda S.P.T., Bartel B.
J. Biol. Chem. 277:20446-20452(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y13577 mRNA. Translation: CAA73905.1.
AF081067 Genomic DNA. Translation: AAC32192.1.
AC025294 Genomic DNA. Translation: AAG50883.1.
CP002684 Genomic DNA. Translation: AEE32712.1.
AF375444 mRNA. Translation: AAK53028.1.
AY143961 mRNA. Translation: AAN28900.1.
PIRF96556.
RefSeqNP_175587.1. NM_104055.3.
UniGeneAt.52138.
At.5375.

3D structure databases

ProteinModelPortalO04373.
SMRO04373. Positions 34-429.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid26827. 1 interaction.
STRING3702.AT1G51760.1-P.

Chemistry

ChEMBLCHEMBL1687678.

Protein family/group databases

MEROPSM20.A04.

Proteomic databases

PaxDbO04373.
PRIDEO04373.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G51760.1; AT1G51760.1; AT1G51760.
GeneID841602.
KEGGath:AT1G51760.

Organism-specific databases

GeneFarm1917. 187.
TAIRAT1G51760.

Phylogenomic databases

eggNOGCOG1473.
HOGENOMHOG000241403.
InParanoidO04373.
KOK14664.
OMAKMLIEPG.
PhylomeDBO04373.

Enzyme and pathway databases

BioCycRETL1328306-WGS:GSTH-3488-MONOMER.

Gene expression databases

GenevestigatorO04373.

Family and domain databases

Gene3D3.30.70.360. 1 hit.
InterProIPR017439. Amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFPIRSF005962. Pept_M20D_amidohydro. 1 hit.
SUPFAMSSF55031. SSF55031. 1 hit.
TIGRFAMsTIGR01891. amidohydrolases. 1 hit.
PROSITEPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameILL4_ARATH
AccessionPrimary (citable) accession number: O04373
Secondary accession number(s): O81642
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: July 9, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names