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O04373

- ILL4_ARATH

UniProt

O04373 - ILL4_ARATH

Protein

IAA-amino acid hydrolase ILR1-like 4

Gene

ILL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (10 Jan 2006)
      Previous versions | rss
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    Functioni

    Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Ala.

    Cofactori

    Manganese. The ion enhances activity.

    GO - Molecular functioni

    1. IAA-Ala conjugate hydrolase activity Source: TAIR

    GO - Biological processi

    1. response to wounding Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Manganese

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-3488-MONOMER.

    Protein family/group databases

    MEROPSiM20.A04.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    IAA-amino acid hydrolase ILR1-like 4 (EC:3.5.1.-)
    Gene namesi
    Name:ILL4
    Synonyms:IAR3, JR3
    Ordered Locus Names:At1g51760
    ORF Names:F19C24.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G51760.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    3. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi206 – 2061S → L in iar3-1; reduces activity. 1 Publication
    Mutagenesisi224 – 2241G → E in iar3-2; abolishes activity. 1 Publication
    Mutagenesisi226 – 2261G → E in iar3-4; reduces activity. 1 Publication
    Mutagenesisi230 – 2301A → T in iar3-3; reduces activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 440417IAA-amino acid hydrolase ILR1-like 4PRO_0000045470Add
    BLAST

    Proteomic databases

    PaxDbiO04373.
    PRIDEiO04373.

    Expressioni

    Tissue specificityi

    Expressed most strongly in roots, stems, cauline leaves and flowers.1 Publication

    Inductioni

    By jasmonic acid (JA) and by wounding.1 Publication

    Gene expression databases

    GenevestigatoriO04373.

    Interactioni

    Protein-protein interaction databases

    BioGridi26827. 1 interaction.
    STRINGi3702.AT1G51760.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO04373.
    SMRiO04373. Positions 34-429.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi437 – 4404Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the peptidase M20 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1473.
    HOGENOMiHOG000241403.
    InParanoidiO04373.
    KOiK14664.
    OMAiKMLIEPG.
    PhylomeDBiO04373.

    Family and domain databases

    Gene3Di3.30.70.360. 1 hit.
    InterProiIPR017439. Amidohydrolase.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view]
    PfamiPF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005962. Pept_M20D_amidohydro. 1 hit.
    SUPFAMiSSF55031. SSF55031. 1 hit.
    TIGRFAMsiTIGR01891. amidohydrolases. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O04373-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFFKWVSFV LILHLLNPTL ISCSSNGLSQ IPSKFLTLAK RNDFFDWMVG    50
    IRRRIHENPE LGYEEVETSK LVRAELEKMG VSYKYPVAVT GVVGYVGTGH 100
    APFVALRADM DALAMQEMVE WEHKSKVPGK MHACGHDAHT TMLLGAAKLL 150
    KEHEEELQGT VVLVFQPAEE GGGGAKKIVE AGVLENVSAI FGLHVTNQLA 200
    LGQVSSREGP MLAGSGFFKA KISGKGGHAA LPQHTIDPIL AASNVIVSLQ 250
    HLVSREADPL DSQVVTVAKF EGGGAFNVIP DSVTIGGTFR AFSTKSFMQL 300
    KKRIEQVITR QASVNMCNAT VDFIEEEKPF FPPTVNDKAL HQFFKNVSGD 350
    MLGIENYVEM QPLMGSEDFS FYQQAIPGHF SFVGMQNKAR SPMASPHSPY 400
    FEVNEELLPY GASLHASMAT RYLLELKAST LNKSNKKDEL 440
    Length:440
    Mass (Da):48,263
    Last modified:January 10, 2006 - v2
    Checksum:i8B605EEF67A1746E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti211 – 2111M → I in CAA73905. (PubMed:9342878)Curated
    Sequence conflicti291 – 2911A → AFST in CAA73905. (PubMed:9342878)Curated
    Sequence conflicti325 – 3295EEEKP → ARGET in CAA73905. (PubMed:9342878)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13577 mRNA. Translation: CAA73905.1.
    AF081067 Genomic DNA. Translation: AAC32192.1.
    AC025294 Genomic DNA. Translation: AAG50883.1.
    CP002684 Genomic DNA. Translation: AEE32712.1.
    AF375444 mRNA. Translation: AAK53028.1.
    AY143961 mRNA. Translation: AAN28900.1.
    PIRiF96556.
    RefSeqiNP_175587.1. NM_104055.3.
    UniGeneiAt.52138.
    At.5375.

    Genome annotation databases

    EnsemblPlantsiAT1G51760.1; AT1G51760.1; AT1G51760.
    GeneIDi841602.
    KEGGiath:AT1G51760.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13577 mRNA. Translation: CAA73905.1 .
    AF081067 Genomic DNA. Translation: AAC32192.1 .
    AC025294 Genomic DNA. Translation: AAG50883.1 .
    CP002684 Genomic DNA. Translation: AEE32712.1 .
    AF375444 mRNA. Translation: AAK53028.1 .
    AY143961 mRNA. Translation: AAN28900.1 .
    PIRi F96556.
    RefSeqi NP_175587.1. NM_104055.3.
    UniGenei At.52138.
    At.5375.

    3D structure databases

    ProteinModelPortali O04373.
    SMRi O04373. Positions 34-429.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 26827. 1 interaction.
    STRINGi 3702.AT1G51760.1-P.

    Chemistry

    ChEMBLi CHEMBL1687678.

    Protein family/group databases

    MEROPSi M20.A04.

    Proteomic databases

    PaxDbi O04373.
    PRIDEi O04373.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G51760.1 ; AT1G51760.1 ; AT1G51760 .
    GeneIDi 841602.
    KEGGi ath:AT1G51760.

    Organism-specific databases

    GeneFarmi 1917. 187.
    TAIRi AT1G51760.

    Phylogenomic databases

    eggNOGi COG1473.
    HOGENOMi HOG000241403.
    InParanoidi O04373.
    KOi K14664.
    OMAi KMLIEPG.
    PhylomeDBi O04373.

    Enzyme and pathway databases

    BioCyci RETL1328306-WGS:GSTH-3488-MONOMER.

    Gene expression databases

    Genevestigatori O04373.

    Family and domain databases

    Gene3Di 3.30.70.360. 1 hit.
    InterProi IPR017439. Amidohydrolase.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view ]
    Pfami PF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005962. Pept_M20D_amidohydro. 1 hit.
    SUPFAMi SSF55031. SSF55031. 1 hit.
    TIGRFAMsi TIGR01891. amidohydrolases. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Jasmonic acid-dependent and -independent signaling pathways control wound-induced gene activation in Arabidopsis thaliana."
      Titarenko E., Rojo E., Leon J., Sanchez-Serrano J.J.
      Plant Physiol. 115:817-826(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    2. "IAR3 encodes an auxin conjugate hydrolase from Arabidopsis."
      Davies R.T., Goetz D.H., Lasswell J.E., Anderson M.N., Bartel B.
      Plant Cell 11:365-376(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, MUTAGENESIS OF SER-206; GLY-224; GLY-226 AND ALA-230.
      Strain: cv. Columbia.
    3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Characterization of a family of IAA-amino acid conjugate hydrolases from Arabidopsis."
      LeClere S., Tellez R., Rampey R.A., Matsuda S.P.T., Bartel B.
      J. Biol. Chem. 277:20446-20452(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.

    Entry informationi

    Entry nameiILL4_ARATH
    AccessioniPrimary (citable) accession number: O04373
    Secondary accession number(s): O81642
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: January 10, 2006
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3