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Protein

IAA-amino acid hydrolase ILR1-like 4

Gene

ILL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Ala, IAA-Asn, IAA-Cys, IAA-Glu, IAA-Met, IAA-Ser and IAA-Gly (PubMed:10072397, PubMed:11923288). Has a lower efficiency with IAA-Phe, IAA-Leu and IAA-Val and no activity with IAA-Ile (PubMed:10072397, PubMed:11923288). Important for IAA-Leu hydrolysis in roots (PubMed:15155875). Also hydrolyzes amino acid conjugates of jasmonic acid and 12-hydroxy jasmonic acid (PubMed:24052260).4 Publications

Catalytic activityi

A jasmonoyl-L-amino acid + H2O = jasmonate + an L-amino acid.1 Publication

Cofactori

Mn2+1 PublicationNote: The Mn2+ ion enhances activity.1 Publication

Kineticsi

kcat is 0.024 sec(-1) with IAA-Ala as substrate.1 Publication
  1. KM=90 µM for IAA-Ala1 Publication
  1. Vmax=20 nmol/min/mg enzyme with IAA-Ala as substrate1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi134Manganese 1 and 2By similarity1
Metal bindingi136Manganese 2By similarity1
Metal bindingi170Manganese 1By similarity1
Metal bindingi194Manganese 2By similarity1
Metal bindingi397Manganese 1By similarity1

GO - Molecular functioni

  • IAA-Ala conjugate hydrolase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • metabolic process Source: InterPro
  • response to wounding Source: TAIR

Keywordsi

Molecular functionHydrolase
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G51760-MONOMER.

Protein family/group databases

MEROPSiM20.A04.

Names & Taxonomyi

Protein namesi
Recommended name:
IAA-amino acid hydrolase ILR1-like 41 Publication (EC:3.5.1.-Curated)
Alternative name(s):
jasmonoyl-L-amino acid hydrolaseCurated (EC:3.5.1.1271 Publication)
Gene namesi
Name:ILL41 Publication
Synonyms:IAR31 Publication, JR31 Publication
Ordered Locus Names:At1g51760Imported
ORF Names:F19C24.4Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G51760.
TAIRilocus:2017607. AT1G51760.

Subcellular locationi

  • Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • plasma membrane Source: TAIR

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi206S → L in iar3-1; reduces activity. 1 Publication1
Mutagenesisi224G → E in iar3-2; abolishes activity. 1 Publication1
Mutagenesisi226G → E in iar3-4; reduces activity. 1 Publication1
Mutagenesisi230A → T in iar3-3; reduces activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1687678.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000004547024 – 440IAA-amino acid hydrolase ILR1-like 4Add BLAST417

Proteomic databases

PaxDbiO04373.
PRIDEiO04373.

Expressioni

Tissue specificityi

Expressed in leaves, stems, roots, siliques and flowers. Detected in the vascular tissue of cotyledons and roots, in adult leaves, stems, siliques, petals, hydathodes and in silique abscission zones and funicles.2 Publications

Inductioni

By jasmonic acid (JA) and by wounding.1 Publication

Gene expression databases

ExpressionAtlasiO04373. baseline and differential.
GenevisibleiO04373. AT.

Interactioni

Protein-protein interaction databases

BioGridi26827. 1 interactor.
STRINGi3702.AT1G51760.1.

Structurei

3D structure databases

ProteinModelPortaliO04373.
SMRiO04373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi437 – 440Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the peptidase M20 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIRP. Eukaryota.
COG1473. LUCA.
HOGENOMiHOG000241403.
InParanoidiO04373.
KOiK14664.
OMAiLAIRTDM.
OrthoDBiEOG09360A3R.
PhylomeDBiO04373.

Family and domain databases

InterProiView protein in InterPro
IPR017439. Amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
PfamiView protein in Pfam
PF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
PIRSFiPIRSF005962. Pept_M20D_amidohydro. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01891. amidohydrolases. 1 hit.
PROSITEiView protein in PROSITE
PS00014. ER_TARGET. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04373-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFFKWVSFV LILHLLNPTL ISCSSNGLSQ IPSKFLTLAK RNDFFDWMVG
60 70 80 90 100
IRRRIHENPE LGYEEVETSK LVRAELEKMG VSYKYPVAVT GVVGYVGTGH
110 120 130 140 150
APFVALRADM DALAMQEMVE WEHKSKVPGK MHACGHDAHT TMLLGAAKLL
160 170 180 190 200
KEHEEELQGT VVLVFQPAEE GGGGAKKIVE AGVLENVSAI FGLHVTNQLA
210 220 230 240 250
LGQVSSREGP MLAGSGFFKA KISGKGGHAA LPQHTIDPIL AASNVIVSLQ
260 270 280 290 300
HLVSREADPL DSQVVTVAKF EGGGAFNVIP DSVTIGGTFR AFSTKSFMQL
310 320 330 340 350
KKRIEQVITR QASVNMCNAT VDFIEEEKPF FPPTVNDKAL HQFFKNVSGD
360 370 380 390 400
MLGIENYVEM QPLMGSEDFS FYQQAIPGHF SFVGMQNKAR SPMASPHSPY
410 420 430 440
FEVNEELLPY GASLHASMAT RYLLELKAST LNKSNKKDEL
Length:440
Mass (Da):48,263
Last modified:January 10, 2006 - v2
Checksum:i8B605EEF67A1746E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211M → I in CAA73905 (PubMed:9342878).Curated1
Sequence conflicti291A → AFST in CAA73905 (PubMed:9342878).Curated1
Sequence conflicti325 – 329EEEKP → ARGET in CAA73905 (PubMed:9342878).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13577 mRNA. Translation: CAA73905.1.
AF081067 Genomic DNA. Translation: AAC32192.1.
AC025294 Genomic DNA. Translation: AAG50883.1.
CP002684 Genomic DNA. Translation: AEE32712.1.
AF375444 mRNA. Translation: AAK53028.1.
AY143961 mRNA. Translation: AAN28900.1.
PIRiF96556.
RefSeqiNP_175587.1. NM_104055.4.
UniGeneiAt.52138.
At.5375.

Genome annotation databases

EnsemblPlantsiAT1G51760.1; AT1G51760.1; AT1G51760.
GeneIDi841602.
GrameneiAT1G51760.1; AT1G51760.1; AT1G51760.
KEGGiath:AT1G51760.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiILL4_ARATH
AccessioniPrimary (citable) accession number: O04373
Secondary accession number(s): O81642
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: June 7, 2017
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families