O04373 (ILL4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
IAA-amino acid hydrolase ILR1-like 4
EC=3.5.1.-

Gene names

Name:	ILL4
Synonyms:	IAR3, JR3
Ordered Locus Names:	At1g51760
ORF Names:	F19C24.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Ala.
Cofactor	Manganese. The ion enhances activity.
Subcellular location	Endoplasmic reticulum lumen Potential.
Tissue specificity	Expressed most strongly in roots, stems, cauline leaves and flowers. Ref.2
Induction	By jasmonic acid (JA) and by wounding. Ref.1
Sequence similarities	Belongs to the peptidase M20 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Ligand	Manganese
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to wounding Inferred from expression pattern PubMed 17675405. Source: TAIR
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 16618929PubMed 22923678. Source: TAIR endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Molecular_function	IAA-Ala conjugate hydrolase activity Inferred from direct assay Ref.2. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 440	417	IAA-amino acid hydrolase ILR1-like 4		PRO_0000045470
Regions
	Motif	437 – 440	4	Prevents secretion from ER Potential
Experimental info
	Mutagenesis	206	1	S → L in iar3-1; reduces activity. Ref.2
	Mutagenesis	224	1	G → E in iar3-2; abolishes activity. Ref.2
	Mutagenesis	226	1	G → E in iar3-4; reduces activity. Ref.2
	Mutagenesis	230	1	A → T in iar3-3; reduces activity. Ref.2
	Sequence conflict	211	1	M → I in CAA73905. Ref.1
	Sequence conflict	291	1	A → AFST in CAA73905. Ref.1
	Sequence conflict	325 – 329	5	EEEKP → ARGET in CAA73905. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O04373 [UniParc]. Last modified January 10, 2006. Version 2. Checksum: 8B605EEF67A1746E	FASTA	440	48,263
	10 20 30 40 50 60 MSFFKWVSFV LILHLLNPTL ISCSSNGLSQ IPSKFLTLAK RNDFFDWMVG IRRRIHENPE 70 80 90 100 110 120 LGYEEVETSK LVRAELEKMG VSYKYPVAVT GVVGYVGTGH APFVALRADM DALAMQEMVE 130 140 150 160 170 180 WEHKSKVPGK MHACGHDAHT TMLLGAAKLL KEHEEELQGT VVLVFQPAEE GGGGAKKIVE 190 200 210 220 230 240 AGVLENVSAI FGLHVTNQLA LGQVSSREGP MLAGSGFFKA KISGKGGHAA LPQHTIDPIL 250 260 270 280 290 300 AASNVIVSLQ HLVSREADPL DSQVVTVAKF EGGGAFNVIP DSVTIGGTFR AFSTKSFMQL 310 320 330 340 350 360 KKRIEQVITR QASVNMCNAT VDFIEEEKPF FPPTVNDKAL HQFFKNVSGD MLGIENYVEM 370 380 390 400 410 420 QPLMGSEDFS FYQQAIPGHF SFVGMQNKAR SPMASPHSPY FEVNEELLPY GASLHASMAT 430 440 RYLLELKAST LNKSNKKDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Jasmonic acid-dependent and -independent signaling pathways control wound-induced gene activation in Arabidopsis thaliana." Titarenko E., Rojo E., Leon J., Sanchez-Serrano J.J. Plant Physiol. 115:817-826(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
[2]	"IAR3 encodes an auxin conjugate hydrolase from Arabidopsis." Davies R.T., Goetz D.H., Lasswell J.E., Anderson M.N., Bartel B. Plant Cell 11:365-376(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, MUTAGENESIS OF SER-206; GLY-224; GLY-226 AND ALA-230. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Characterization of a family of IAA-amino acid conjugate hydrolases from Arabidopsis." LeClere S., Tellez R., Rampey R.A., Matsuda S.P.T., Bartel B. J. Biol. Chem. 277:20446-20452(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y13577 mRNA. Translation: CAA73905.1. AF081067 Genomic DNA. Translation: AAC32192.1. AC025294 Genomic DNA. Translation: AAG50883.1. CP002684 Genomic DNA. Translation: AEE32712.1. AF375444 mRNA. Translation: AAK53028.1. AY143961 mRNA. Translation: AAN28900.1.
IPI	IPI00517189.
PIR	F96556.
RefSeq	NP_175587.1. NM_104055.3.
UniGene	At.52138. At.5375.
3D structure databases
ProteinModelPortal	O04373.
SMR	O04373. Positions 34-429.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G51760.1-P.
Protein family/group databases
MEROPS	M20.A04.
Proteomic databases
PaxDb	O04373.
PRIDE	O04373.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G51760.1; AT1G51760.1; AT1G51760.
GeneID	841602.
KEGG	ath:AT1G51760.
Organism-specific databases
GeneFarm	1917. 187.
TAIR	At1g51760.
Phylogenomic databases
eggNOG	COG1473.
HOGENOM	HOG000241403.
InParanoid	O04373.
KO	K14664.
OMA	YFTINED.
PhylomeDB	O04373.
ProtClustDB	CLSN2679431.
Gene expression databases
Genevestigator	O04373.
GermOnline	AT1G51760. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR017439. Amidohydrolase. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. [Graphical view]
Pfam	PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view]
PIRSF	PIRSF005962. Pept_M20D_amidohydro. 1 hit.
SUPFAM	SSF55031. Peptidase_M20_dimer. 1 hit.
TIGRFAMs	TIGR01891. amidohydrolases. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1687678.

Entry information

Entry name

ILL4_ARATH

Accession

Primary (citable) accession number: O04373
Secondary accession number(s): O81642

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	January 10, 2006
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program