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Protein

rRNA N-glycosidase

Gene
N/A
Organism
Sambucus nigra (European elder)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.UniRule annotationSAAS annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationSAAS annotation, Protein synthesis inhibitorUniRule annotationSAAS annotation, ToxinUniRule annotationSAAS annotation

Keywords - Biological processi

Plant defenseUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA N-glycosidaseUniRule annotation (EC:3.2.2.22UniRule annotation)
OrganismiSambucus nigra (European elder)Imported
Taxonomic identifieri4202 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsDipsacalesAdoxaceaeSambucus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 563538rRNA N-glycosidaseSequence analysisPRO_5004156728Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO04367.
SMRiO04367. Positions 26-276, 299-560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini306 – 431126Ricin B-type lectinInterPro annotationAdd
BLAST
Domaini434 – 559126Ricin B-type lectinInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ribosome-inactivating protein family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00652. Ricin_B_lectin. 2 hits.
PF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SMARTiSM00458. RICIN. 2 hits.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 2 hits.
SSF56371. SSF56371. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 2 hits.
PS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O04367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVIAAAMLY LYIVVLAICS VGIQGIDYPS VSFNLAGAKS ATYRDFLKNL
60 70 80 90 100
RTIVATGTYE VNGLPVLRRE SEVQVKNRFV LVLLTNYNGN TVTLAVDVTN
110 120 130 140 150
LYVVAFSANA NSYFFKDATQ LQKSNLFVGT RQHTLPFTGN YDNLETAAGT
160 170 180 190 200
RRESIELGPS PLDGAITSLY YDESVARSLL VVIQMVSEAA RFRYIEQEVR
210 220 230 240 250
RSLQQTAGFT PNALMLSMEN NWSSMSLEVQ QSGDNVSPFT GTVQLQNYDH
260 270 280 290 300
TPRLVDNFEE LYKITGIAIL LFRCFSPSND SAIRRPHVLA GEDNKYNDGE
310 320 330 340 350
TCPIPASFTR RIVGRDGLCV DVRDGYDTDG TPIQLWPCGS QRNQQWTFHK
360 370 380 390 400
DDTIRSMGKC MTANGFNSGS SIMIFNCSTA VKNATKWEVP IDGSIINPSS
410 420 430 440 450
GRVMTAPSAA SRTILLLQNN IYAASQGWTV SNDVQPVVAS IVGYKEMCLQ
460 470 480 490 500
ANGENNGVWM ENCEVTSLQQ QWALFGDRTI RVNSDRGLCV TTNGYHSKDL
510 520 530 540 550
IIILKCQGLP SQRWFFNSNG AIVNPNSTLV MDVKANDVSL REIIIFPSHG
560
DPNQQWVTQV LPS
Length:563
Mass (Da):62,337
Last modified:July 1, 1997 - v1
Checksum:i3ED2B6C08E796205
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76524 mRNA. Translation: AAC15886.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76524 mRNA. Translation: AAC15886.1.

3D structure databases

ProteinModelPortaliO04367.
SMRiO04367. Positions 26-276, 299-560.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00652. Ricin_B_lectin. 2 hits.
PF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SMARTiSM00458. RICIN. 2 hits.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 2 hits.
SSF56371. SSF56371. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 2 hits.
PS00275. SHIGA_RICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The major elderberry (Sambucus nigra) fruit protein is a lectin derived from a truncated type 2 ribosome-inactivating protein."
    Van Damme E.J., Roy S., Barre A., Rouge P., Van Leuven F., Peumans W.J.
    Plant J. 12:1251-1260(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiO04367_SAMNI
AccessioniPrimary (citable) accession number: O04367
Entry historyi
Integrated into UniProtKB/TrEMBL: July 1, 1997
Last sequence update: July 1, 1997
Last modified: February 17, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.