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Protein

rRNA N-glycosidase

Gene
N/A
Organism
Iris hollandica (Dutch iris) (Iris tingitana x Iris xiphium)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.UniRule annotationSAAS annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationSAAS annotation, Protein synthesis inhibitorUniRule annotationSAAS annotation, ToxinUniRule annotationSAAS annotation

Keywords - Biological processi

Plant defenseUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA N-glycosidaseUniRule annotation (EC:3.2.2.22UniRule annotation)
OrganismiIris hollandica (Dutch iris) (Iris tingitana x Iris xiphium)Imported
Taxonomic identifieri35876 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesIridaceaeIris

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 298275rRNA N-glycosidaseSequence analysisPRO_5004156952Add
BLAST

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MVGX-ray1.25A/B24-298[»]
ProteinModelPortaliO04358.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO04358.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosome-inactivating protein family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.

Sequencei

Sequence statusi: Complete.

O04358-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMWLILAVT WILWATIVGP VASIETVEFR VTGTTRRSYS DFLQNLRNRL
60 70 80 90 100
SSGTSVHDIP LLPAQSGSQQ DLLFVRLFDW GNRPITLVLN RVNAYVVAYQ
110 120 130 140 150
AQNRFYLLAD TPANPQVYGN NPHRLTFIGS YGALQNVAKQ NRENIDLGIN
160 170 180 190 200
PLATAISTLH NWSPPTVETS VARSLIVLIQ LVSETARFRA IEQRVTNNII
210 220 230 240 250
DQVTPIRYDN FRPSVGIIDL QTNWQTLSTE VQRAEGGRFL QPVKLQVSVQ
260 270 280 290
QTVVISDVEK ARTFCGLALL LRWRPSQASL SSHDFAWRDI RSILDIGA
Length:298
Mass (Da):33,568
Last modified:July 1, 1997 - v1
Checksum:i0D91447CE2A021B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78041 mRNA. Translation: AAC49781.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78041 mRNA. Translation: AAC49781.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MVGX-ray1.25A/B24-298[»]
ProteinModelPortaliO04358.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO04358.

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Van Damme E.J.M.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: ShootImported.
  2. "Type 1 ribosome-inactivating proteins are the most abundant proteins in iris (Iris hollandica var. Professor Blaauw) bulbs: characterization and molecular cloning."
    Van Damme E.J., Barre A., Barbieri L., Valbonesi P., Rouge P., Van Leuven F., Stirpe F., Peumans W.J.
    Biochem. J. 324:963-970(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: ShootImported.
  3. "Native structure of IRIP, a type I ribosome inactivating protein from Iris hollandica var. at 1.25 A."
    Meyer A., Weber W., Singh T.P., Betzel C.
    Submitted (MAY-2010) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.25 ANGSTROMS) OF 24-298.

Entry informationi

Entry nameiO04358_IRIHO
AccessioniPrimary (citable) accession number: O04358
Entry historyi
Integrated into UniProtKB/TrEMBL: July 1, 1997
Last sequence update: July 1, 1997
Last modified: February 17, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.